Nfatc2 (NM_001291172) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; Nfatp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230796 representing NM_001291172 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCGCCCCGCATTGCGCAGGCCGGGCCCCCGCCAGCCCCTCCGCACCATGGGGTCTGCGGACCGAG AAGAACCGATCGCACATAAGGCCATCAGCTCACCCTCCGGACTCGCATACCCGGATGATGTCCTGGACTA TGGCCTCAAGCCATGCAACCCCCTTGCCAGTCTCTCTGGCGAGCCCCCTGGCCGGTTCGGAGAGCCGGAT AGTATAGGGTTCCAGAACTTTCTGAGCCCGGTCAAGCCAGCAGGGGCTTCGGGCCCGAGCCCTCGGATCG AGATCACTCCATCCCACGAACTGATGCAGGCAGGGGGGGCCCTCCGTGGGAGAGACGCCGGCCTGTCCCC CGAGCAGCCGGCCCTGGCCCTGGCCGGCGTGGCCGCCAGCCCGAGGTTCACACTGCCCGTGCCCGGCTAC GAGGGCTACCGCGAGCCGCTTTGCTTGAGCCCCGCTAGCAGCGGCTCCTCTGCCAGCTTCATTTCTGACA CCTTCTCCCCCTACACCTCGCCCTGCGTCTCACCCAATAACGCCGGGCCCGACGACCTGTGTCCCCAGTT TCAAAACATCCCTGCTCATTATTCCCCCAGAACCTCTCCAATAATGTCACCTCGAACCAGCCTCGCCGAG GACAGCTGCCTGGGCCGACACTCGCCCGTGCCCCGTCCGGCATCCCGCTCCTCCTCACCCGGTGCCAAGC GGAGGCATTCGTGCGCAGAGGCTTTGGTTGCTCCTCTGCCCGCAGCCTCACCCCAGCGCTCCCGGAGCCC CTCGCCACAGCCCTCGCCTCACGTGGCACTGCAGGACGACAGCATCCCCGCTGGGTACCCCCCCACGGCC GGCTCTGCTGTTCTCATGGATGCCCTCAACACCCTGGCCACCGACTCGCCCTGCGGGATCCCCTCCAAGA TATGGAAGACCAGTCCTGACCCGACGCCTGTGTCCACCGCTCCGTCCAAGGCTGGCCTGGCCCGCCACAT CTACCCTACTGTGGAGTTCCTGGGGCCATGTGAGCAGGAGGAGAGGAGGAATTCCGCTCCAGAGTCCATC CTGCTGGTACCACCTACTTGGCCCAAGCAGTTGGTGCCGGCCATTCCCATCTGCAGCATCCCTGTGACTG CATCCCTCCCACCACTCGAGTGGCCACTCTCCAATCAGTCGGGCTCCTATGAGCTACGGATTGAGGTCCA ACCCAAGCCCCATCACCGGGCCCACTATGAGACGGAGGGCAGCCGTGGCGCTGTCAAAGCCCCAACAGGA GGACACCCTGTGGTGCAGCTCCACGGCTACATGGAGAACAAGCCTCTGGGGCTTCAGATCTTCATTGGGA CAGCAGATGAGAGGATCCTTAAGCCGCACGCCTTCTACCAAGTACACAGGATCACTGGGAAAACGGTCAC CACCACGAGCTATGAGAAGATCGTAGGCAACACCAAGGTCCTGGAGATCCCCCTGGAGCCAAAGAACAAC ATGAGAGCCACCATCGACTGTGCAGGCATCCTGAAGCTCCGAAACGCTGACATCGAGCTGCGGAAGGGCG AGACGGACATCGGCAGGAAGAACACGCGTGTGCGCCTGGTGTTCCGCGTGCACGTCCCAGAGCCCAGTGG GCGCATCGTCTCCCTGCAGGCTGCGTCCAACCCCATCGAGTGCTCTCAGCGCTCTGCCCACGAGCTGCCC ATGGTGGAGAGACAAGACATGGACAGCTGCCTGGTCTACGGGGGCCAGCAGATGATCCTCACGGGCCAGA ACTTCACAGCGGAGTCCAAGGTTGTGTTCATGGAGAAGACTACAGGACCTGCAGGGACCTGTGAGACTCG GCCTTTGCCCATCTCTCTGATCTCAGCTGACCGTCTCTCCCCCTGGCTTTCCCGGCTGCAGAGAAACCCT CCTGGCTCTGTCTTCAGATGCTCCGTGCTCCTTCCTGCCCCAGGATCTTCGCTTGTGCTGCTGGCTCTG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230796 representing NM_001291172 Red=Cloning site Green=Tags(s) MQRPALRRPGPRQPLRTMGSADREEPIAHKAISSPSGLAYPDDVLDYGLKPCNPLASLSGEPPGRFGEPD SIGFQNFLSPVKPAGASGPSPRIEITPSHELMQAGGALRGRDAGLSPEQPALALAGVAASPRFTLPVPGY EGYREPLCLSPASSGSSASFISDTFSPYTSPCVSPNNAGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAE DSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVAPLPAASPQRSRSPSPQPSPHVALQDDSIPAGYPPTA GSAVLMDALNTLATDSPCGIPSKIWKTSPDPTPVSTAPSKAGLARHIYPTVEFLGPCEQEERRNSAPESI LLVPPTWPKQLVPAIPICSIPVTASLPPLEWPLSNQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTG GHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNN MRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHVPEPSGRIVSLQAASNPIECSQRSAHELP MVERQDMDSCLVYGGQQMILTGQNFTAESKVVFMEKTTGPAGTCETRPLPISLISADRLSPWLSRLQRNP PGSVFRCSVLLPAPGSSLVLLAL TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291172
ORF Size	1959 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291172.1, NP_001278101.1
RefSeq Size	3283 bp
RefSeq ORF	1962 bp
Locus ID	18019
MW	70.4 kDa
Gene Summary	Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.