Ncaph2 (NM_001271601) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D15Ertd785
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230664 representing NM_001271601 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGTGGAGGTGCGCTTTGCTCACCTCTTGCAGCCCATCCGGGATCTCACTAAGAACTGGGAGG TGGACGTGGCGGCACAGCTGGGCGAGTATCTGGAGGAGCTGGACCAGATCTGCATTTCTTTTGATGAAGG CAAAACCACAATGAACTTCATTGAGGCAGCACTGTTGATCCAGGGCTCAGCCTGTGTCTACAGTAAGAAG GTGGAGTACCTCTACTCGCTGGTCTACCAGGCTCTCGATTTTATTTCTGGCAAGAGGCGGGCCAAACAGC TCTCCTTAGTTCAGGAAGATGGGAGCAAGAAGACTGTCAACTCAGAGACTCCCTGTGAAACAGAGAATGA GTTCCTGTCACTGGACGACTTCCCTGACTCCCGGGCTAATGTGGATCTGAAAAATGATCAGGCATCCAGT GAGCTGCTTATCATACCCCTACTGCCCATGGCCCTGGTGGCCCCTGATGAAGTGGAAAAGAACAGCAGCC CCTTGTATAGCTGTCAGGGTGACATCTTGGCCAGCCGGAAGGATTTCAGGATGAACACGTGTATGCCTAA CCCCAGAGGCTGCTTTATGTTAGATCCAGTGGGAATGTGTCCTGTGGAGCCTGTGGTGCCCGTGGAGCCA TACCCCATGTCAAGGAGCCAGAAAGATCCTGAGGACGCTGAGGAGCAGCCCATGGAAGTGTCTAGGAACG GGAGTCCTGTTCCTGTACCCGACATCTCCCAAGAGCCAGATGGTCCAGCGCTCAGCGGTGGAGAGGAGGA TGCAGAGGATGGAGCAGAGCCCCTGGAGGTTGCTCTAGAGCCTGCAGAGCCAAGGACCTCACAGCAGAGT GCCATCTTGCCAAGGAGATACATGCTGCGGGAACGACAAGGGGCACCGGAGCCTGCCTCCCGGCTACAGG AGACCCCAGACCCCTGGCAGAGCCTGGACCCTTTTGACTCCTTGGAATCTAAGGTCTTCCAGAAAGGGAA ACCCTATTCTGTGCCACCCGGTGTGGAGGAGGCTCCAGGACAGAAGCGCAAGAGGAAGGGTGCCACCAAG TTGCAGGACTTCCACAAGTGGTACCTGGATGCCTATGCTGAACACCCTGACGGCAGGAGGGCTCGGCGGA AGGGCCCAACCTTTGCAGACATGGAAGTCCTGTACTGGAAACATGTGAAAGAACAGCTCGAGACCCTTCA GAAGCTGCGGAGACGTAAGATCAACGAGAGATGGCTACCTGGGGCCAAGCAGGATCTGTGGCCTACAGAG GAGGATCGCTTGGAAGAGTCCCTCGAGGACCTCGGGGTAGCAGCAGATGACTTTCTAGAGCCCGAGGAGT ACGTGGAGGAGCCTGCGGGGGTGATGCCCGAGGAAGCTGCTGACCTCGATGCAGAGGCCATGCCAGAGTC CCTGAGATACGAGGAGCTGGTCCGGAGAAATGTGGAACTCTTCATTGCCACCTCCCAGAAGTTTATCCAG GAGACAGAGCTGAGCCAACGCATCAGGGACTGGGAAGATACCATCCAGCCCCTGCTCCAGGAGCAGGAGC AGCATGTGCCCTTTGATATCCATATCTACGGGGACCAGTTGGCTTCACGGTTCCCCCAGCTCAATGAATG GTGTCCCTTTTCAGAGCTTGTAGCAGGGCAGCCTGCTTTTGAGGTGTGCCGCTCCATGCTGGCCTCCTTG CAACTGGCTAATGACTACACAGTGGAGATCACTCAGCAGCCAGGACTGGAGGCAGCTGTGGACACAATGT CTCTGAGACTGCTCACACACCAGCGAGCCCACACCCGCTTCCAGACCTATGCTGCACCATCCATGGCCCA GCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230664 representing NM_001271601 Red=Cloning site Green=Tags(s) MEDVEVRFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISFDEGKTTMNFIEAALLIQGSACVYSKK VEYLYSLVYQALDFISGKRRAKQLSLVQEDGSKKTVNSETPCETENEFLSLDDFPDSRANVDLKNDQASS ELLIIPLLPMALVAPDEVEKNSSPLYSCQGDILASRKDFRMNTCMPNPRGCFMLDPVGMCPVEPVVPVEP YPMSRSQKDPEDAEEQPMEVSRNGSPVPVPDISQEPDGPALSGGEEDAEDGAEPLEVALEPAEPRTSQQS AILPRRYMLRERQGAPEPASRLQETPDPWQSLDPFDSLESKVFQKGKPYSVPPGVEEAPGQKRKRKGATK LQDFHKWYLDAYAEHPDGRRARRKGPTFADMEVLYWKHVKEQLETLQKLRRRKINERWLPGAKQDLWPTE EDRLEESLEDLGVAADDFLEPEEYVEEPAGVMPEEAADLDAEAMPESLRYEELVRRNVELFIATSQKFIQ ETELSQRIRDWEDTIQPLLQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFSELVAGQPAFEVCRSMLASL QLANDYTVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271601
ORF Size	1824 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271601.1, NP_001258530.1
RefSeq Size	3343 bp
RefSeq ORF	1827 bp
Locus ID	52683
UniProt ID	Q8BSP2
MW	69.5 kDa
Gene Summary	This gene encodes a component of the condensin-2 complex. The encoded protein may regulate the structure of mitotic chromosomes. Loss of function of this gene disrupts T-cell development. There are two pseudogenes for this gene on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

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