Traf3 (NM_001286122) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1; T-BAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230451 representing NM_001286122 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAAGCAAAAAGATGGATGCTGCTGGCACACTGCAGCCTAACCCACCCCTAAAGCTGCAGCCTG ATCGCGGCGCAGGGTCCGTGCTCGTGCCGGAGCAAGGAGGCTACAAGGAGAAGTTTGTGAAGACGGTGGA AGACAAGTACAAGTGCGAGAAGTGCCGCCTGGTGCTGTGCAACCCGAAGCAGACGGAGTGTGGCCACCGG TTCTGCGAGAGCTGCATGGCCGCCCTGCTGAGCTCCTCCAGTCCAAAATGCACAGCGTGCCAAGAAAGCA TCATCAAAGACAAGGTGTTTAAGGATAATTGCTGCAAGAGAGAGATTCTGGCCCTTCAGGTCTACTGTCG GAATGAAGGCAGAGGTTGTGCGGAGCAGCTGACTCTGGGACATCTGCTGGTGCACCTAAAAAATGAATGT CAGTTTGAGGAACTTCCCTGTCTGCGTGCCGACTGCAAAGAAAAAGTACTGAGAAAAGACTTGCGGGATC ACGTGGAAAAGGCCTGTAAATACCGCGAGGCCACGTGCAGTCACTGCAAGAGCCAAGTGCCCATGATCAA ACTGCAGAAACATGAAGACACAGATTGTCCCTGTGTGGTGGTATCCTGCCCTCACAAGTGCAGCGTTCAG ACTCTTCTAAGGAGTGAGGGTACAAACCAGCAGATCAAGGCCCATGAGGCCAGCTCCGCGGTACAGCACG TGAACCTGCTGAAGGAGTGGAGCAACTCCCTGGAGAAGAAGGTTTCCCTGCTGCAGAATGAAAGTGTTGA GAAAAACAAGAGCATCCAAAGCCTGCACAACCAGATCTGCAGCTTTGAGATCGAGATTGAGAGGCAGAAG GAGATGCTCCGAAACAACGAGTCCAAGATCCTTCACCTGCAGCGGGTAATCGACAGCCAAGCAGAGAAAC TGAAAGAACTGGACAAGGAGATCCGTCCCTTCCGGCAGAACTGGGAGGAAGCGGACAGCATGAAGAGCAG TGTGGAGTCCCTCCAGAACCGAGTGACTGAGCTGGAGAGCGTAGACAAAAGTGCGGGGCAGGCGGCTCGC AACACAGGCTTGCTGGAGTCCCAGCTGAGCCGGCATGACCAGATGTTGAGTGTTCATGACATCCGCTTGG CCGACATGGACCTGCGGTTCCAGGTCCTCGAGACCGCCAGCTACAACGGGGTGCTGATCTGGAAGATCCG TGACTACAAGCGCCGGAAGCAGGAGGCCGTCATGGGGAAGACCCTGTCTCTCTACAGCCAGCCTTTCTAC ACAGGTTATTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAATGGGGACGGAATGGGGAAAGGGACAC ACTTGTCGCTGTTTTTTGTCATTATGCGTGGAGAATATGATGCTCTGTTGCCATGGCCGTTCAAGCAGAA AGTGACACTTATGCTGATGGATCAGGGGTCCTCTCGCCGTCATCTGGGAGATGCGTTCAAGCCTGACCCC AACAGCAGCAGCTTCAAGAAACCCACCGGAGAGATGAATATCGCCTCTGGCTGCCCAGTCTTTGTCGCCC AAACTGTTCTAGAGAACGGGACGTATATTAAAGATGATACAATCTTTATTAAGGTCATAGTGGATACCTC GGATCTGCCTGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230451 representing NM_001286122 Red=Cloning site Green=Tags(s) MESSKKMDAAGTLQPNPPLKLQPDRGAGSVLVPEQGGYKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHR FCESCMAALLSSSSPKCTACQESIIKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNEC QFEELPCLRADCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIKLQKHEDTDCPCVVVSCPHKCSVQ TLLRSEGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQK EMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQAAR NTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFY TGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDP NSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286122
ORF Size	1626 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286122.1, NP_001273051.1
RefSeq Size	6978 bp
RefSeq ORF	1629 bp
Locus ID	22031
MW	61.6 kDa
Gene Summary	Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.[UniProtKB/Swiss-Prot Function]

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