Eya2 (NM_001271963) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Eab1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230412 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGAAGTGGTGACCTCACCCAGCCTCGCAACAAGCAGTGACTGGAGCGAGCACGGTGCTGCCGTGG GGACGCTGAGTGACAGGGAAGGCATCGCCAAATCAGCGGCTCTGAGTGTGCCTCAGCTCTTTGTGAAGTC TCATCCACGTGTCCCTCCTGGTCAGTCCTCCACAGCCATGGCGGCCTATGGCCAGACACAGTACAGCACA GGCATTCAGCAGGCACCACCCTATACAGCGTACCCAACTCCGGCGCAAGCCTATGGAATCCCCCCTTACA GCATCAAGACAGAAGACAGTTTGAATCACTCCCCCAGCCAGAGCGGGTTCCTGAGCTATGGACCGAGCTT CAGCACCGCGCCTGCTGGACAGAGCCCCTACACCTACCCCGTGCACAGCACCGCTGGGCTCTATCAAGGC GCCAACGGACTGACCAACACCGCTGGATTTGGGAGCGTGCACCAGGATTATCCGTCCTACCCCAGCTTTT CACAGAACCAGTACCCCCAGTATTTCAGCCCATCATACAACCCGCCCTACGTCCCTGCCAGCAGCCTCTG CTCCTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCTCCTCACAATGTCCCCAGCCAGAGTTCT GAGTCCCTGGCCGGAGACTACAACACACACAACGGACCCTCCACACCAGCAAAGGAGGGTGACACAGAGA GGCCACATCGAGCCTCGGATGGGAAGCTACGGGGCCGGTCAAAGAGAAATAGTGACCCTTCCCCAGCAGG AGACAATGAAATCGAGCGCGTGTTCGTCTGGGACCTGGACGAGACAATCATTATCTTCCACTCCCTGCTC ACAGGGACGTTTGCATCCAGATACGGGAAGGACACCACGACGTCTGTGCGCATTGGCCTGATGATGGAGG AGATGATCTTCAACCTTGCTGACACACACCTGTTCTTCAATGACCTGGAGGACTGTGACCAAATCCACGT GGATGATGTCTCATCCGATGACAATGGTCAGGATTTAAGCACATACAACTTCTCCACTGATGGCTTCCAC AGCACGGCGCCAGGAGCCAGCTTGTGCCTGGGTACAGGTGTTCATGGCGGTGTGGACTGGATGAGGAAAC TGGCCTTCCGCTACCGTCGTGTGAAGGAGATGTACAACACCTACCGCAACAACGTGGGTGGCTTGATAGG TGCTCCCAAAAGAGAGACCTGGCTGCAGCTGCGCGCCGAGCTGGAGGCCCTGACTGACCTCTGGCTCACC CACTCCCTGAAAGCCCTCAATCTCATCAACTCTCGACCCAACTGTGTCAATGTGTTGGTCACCACCACGC AACTGATCCCTGCATTGGCCAAGGTCCTGCTGTACGGTCTGGGCTCCGTGTTCCCCATCGAGAACATCTA CAGTGCGACCAAGACAGGCAAGGAGAGCTGCTTCGAAAGAATCATGCAGAGGTTTGGCCGCAAAGCTGTC TACATTGTGATAGGCGACGGGGTAGAGGAAGAGCAAGGAGCCAAAAAGCACAACATGCCTTTCTGGAGGA TATCCTGTCATGCTGACCTGGAGGCTCTAAGGCATGCCCTGGAACTGGAGTATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230412 protein sequence Red=Cloning site Green=Tags(s) MLEVVTSPSLATSSDWSEHGAAVGTLSDREGIAKSAALSVPQLFVKSHPRVPPGQSSTAMAAYGQTQYST GIQQAPPYTAYPTPAQAYGIPPYSIKTEDSLNHSPSQSGFLSYGPSFSTAPAGQSPYTYPVHSTAGLYQG ANGLTNTAGFGSVHQDYPSYPSFSQNQYPQYFSPSYNPPYVPASSLCSSPLSTSTYVLQEAPHNVPSQSS ESLAGDYNTHNGPSTPAKEGDTERPHRASDGKLRGRSKRNSDPSPAGDNEIERVFVWDLDETIIIFHSLL TGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSTDGFH STAPGASLCLGTGVHGGVDWMRKLAFRYRRVKEMYNTYRNNVGGLIGAPKRETWLQLRAELEALTDLWLT HSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAV YIVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271963
ORF Size	1596 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271963.1, NP_001258892.1
RefSeq Size	2459 bp
RefSeq ORF	1599 bp
Locus ID	14049
UniProt ID	O08575
MW	58.2 kDa
Gene Summary	This gene encodes a member of the eyes absent protein family. The encoded protein is a tyrosine phosphatase which acts as a transcriptional activator during development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

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