Ppp3ca (NM_001293622) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900074D19Rik; Caln; Calna; CN; CnA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230318 representing NM_001293622 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGAGCCCAAGGCGATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCCGTTCCATTTC CACCAAGTCACCGGCTGACAGCAAAGGAAGTGTTTGATAATGATGGGAAACCTCGTGTGGATATCTTAAA AGCACATCTCATGAAGGAGGGCAGGCTGGAAGAAAGTGTTGCATTGAGAATAATAACAGAGGGTGCTTCG ATTCTCCGACAGGAAAAAAACTTGCTGGATATCGACGCACCAGTCACAGTTTGTGGGGACATCCATGGAC AATTCTTTGACTTGATGAAGCTCTTTGAAGTGGGAGGATCTCCTGCCAACACTCGCTACCTCTTCTTAGG GGACTATGTTGACAGAGGGTACTTCAGTATCGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTTTAC CCCAAAACACTGTTTTTACTTCGCGGAAACCATGAATGTAGGCACCTCACAGAGTATTTCACGTTTAAAC AAGAATGTAAAATAAAGTATTCAGAACGCGTTTATGACGCCTGTATGGATGCCTTCGACTGCCTTCCCCT GGCTGCGCTAATGAACCAGCAGTTCCTGTGTGTACACGGTGGTTTGTCTCCAGAGATTAACACTCTAGAT GACATCAGAAAATTAGACCGATTCAAAGAACCACCTGCTTATGGGCCCATGTGTGACATCCTATGGTCAG ACCCCCTGGAGGACTTTGGAAATGAGAAGACTCAGGAACATTTCACTCACAACACAGTCAGAGGCTGTTC GTACTTCTACAGTTACCCAGCTGTGTGTGACTTCCTGCAGCACAATAATTTGTTGTCCATACTCCGCGCC CACGAAGCCCAGGATGCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCGTCTCTAATTA CAATCTTCTCGGCACCAAATTACTTAGATGTGTACAATAACAAAGCTGCAGTGTTGAAGTACGAGAACAA TGTGATGAACATCAGGCAGTTCAACTGCTCCCCGCATCCGTACTGGCTCCCAAATTTCATGGATGTTTTC ACCTGGTCGCTGCCATTTGTTGGGGAGAAAGTGACTGAGATGCTGGTCAATGTTCTCAACATCTGCTCCG ACGATGAACTGGGGTCAGAAGAAGATGGATTTGACGGAGCCACGGCCGCAGCCCGGAAGGAAGTCATCAG AAACAAGATCCGAGCAATAGGCAAAATGGCCAGAGTGTTCTCAGTTCTCAGAGAAGAGAGTGAGAGTGTC CTGACACTGAAGGGCCTGACCCCAACTGGCATGCTCCCCAGCGGAGTGCTCTCTGGCGGGAAACAGACTC TGCAAAGCGCCATCAAAGGATTTTCACCACAACATAAGATCACTAGCTTCGAGGAGGCCAAGGGCTTAGA CCGAATTAACGAGAGGATGCCACCTCGCAGAGACGCCATGCCCTCTGACGCCAACCTTAACTCCATCAAC AAGGCTCTCGCCTCAGAGACTAACGGCACGGACAGCAATGGCAGTAATAGCAGCAATATCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230318 representing NM_001293622 Red=Cloning site Green=Tags(s) MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGAS ILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILY PKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLD DIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCDFLQHNNLLSILRA HEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVF TWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESV LTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN KALASETNGTDSNGSNSSNIQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293622
ORF Size	1533 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293622.1, NP_001280551.1
RefSeq Size	4832 bp
RefSeq ORF	1536 bp
Locus ID	19055
UniProt ID	P63328
MW	58.1 kDa
Gene Summary	Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:7791792, PubMed:26794871). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (By similarity). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (By similarity). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (By similarity). Dephosphorylates and inactivates transcription factor ELK1 (By similarity). Dephosphorylates DARPP32 (By similarity). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (By similarity).[UniProtKB/Swiss-Prot Function]

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