Micu1 (NM_001291442) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C730016L05Rik; Calc; Cbara1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230144 representing NM_001291442 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGTCTTAACACCCTTTCTGCGTTGGCAGAACTAGCTGTGGGCTCTCGCTGGTACCATGGGGCAT CACAGCCCACACAGACCAAACGAAGATTGATGCTGGTGGCGTTCCTGGGAGCATCTGCAGTGACTGCAAG TACCGGTCTCCTGTGGAAGAAGGCTCACGCAGAATCTCCACCATGCGTTAACAGCAAGAAGCCTGACACA GAAGATAAAGAGAGAAACAAAGACAGCGGGGAAGTGTCCAGCCGTGAAGGAAGGGCTGCAGATGCTGCGG CAGAGCCTTATCCTGAGGACAAGAAGAAGAAGCGCTCTGGATTCAGAGACAGAAAAGTGATGGAGTATGA GAATCGAATCCGAGCCTACTCCACACCAGACAAAATTTTCCGATATTTTGCCACCTTGAAAGTAATCAAC GAACCTGGTGAAACCGAAGTGTTCATGACTCCGCAGGACTTTGTGCGCTCCATAACGCCCAATGAGAAGC AGCCAGAACACTTGGGCCTGGATCAGTATATAATAAAGCGCTTTGATGGAAAGGAGTTCTGGCAGACCGA GAAAATTGCCCAGGAACGAGAAAAATTTGCTGATGAAGGCAGCATCTTCTACAGCCTTGGAGAGTGTGGG CTCATCTCCTTCTCTGACTACATCTTCCTCACAACAGTCCTCTCCACTCCTCAGAGAAATTTTGAAATTG CTTTCAAGATGTTTGACTTGAATGGAGACGGAGAGGTAGACATGGAGGAGTTTGAGCAGGTTCAGAGCAT CATTCGCTCCCAGACCAGCATGGGCATGCGTCACAGAGACCGTCCAACCACTGGGAACACCCTCAAGTCT GGCTTATGTTCGGCCCTCACGACCTACTTTTTTGGAGCTGATCTCAAAGGGAAACTGACCATCAAAAACT TCCTGGAATTTCAGCGCAAACTTCAGCATGACGTTCTAAAGCTGGAGTTTGAACGCCATGACCCGGTAGA TGGGAGAATCTCTGAGAGGCAGTTCGGGGGCATGCTGCTGGCCTACAGTGGAGTGCAGTCCAAGAAGCTG ACCGCCATGCAGAGACAGCTGAAGAAGCACTTCAAAGATGGGAAGGGCCTGACTTTCCAGGAGGTAGAGA ACTTCTTCACTTTCCTGAAGAACATTAATGACGTGGACACTGCACTAAGCTTTTACCACATGGCTGGAGC ATCTCTTGATAAAGTGACCATGCAGCAAGTGGCCAGGACAGTGGCGAAGGTCGAACTCTCGGACCATGTG TGTGACGTGGTGTTCGCGCTCTTTGACTGTGATGGCAATGGGGAGCTGAGCAACAAGGAGTTCGTTTCCA TCATGAAGCAGCGGCTGATGAGAGGCCTGGAGAAGCCCAAGGACATGGGCTTCACCCGCCTCATGCAGGC CATGTGGAAATGTGCCCAAGAAACTGCCTGGGACTTTGCTCTGCCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230144 representing NM_001291442 Red=Cloning site Green=Tags(s) MFRLNTLSALAELAVGSRWYHGASQPTQTKRRLMLVAFLGASAVTASTGLLWKKAHAESPPCVNSKKPDT EDKERNKDSGEVSSREGRAADAAAEPYPEDKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVIN EPGETEVFMTPQDFVRSITPNEKQPEHLGLDQYIIKRFDGKEFWQTEKIAQEREKFADEGSIFYSLGECG LISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKS GLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRISERQFGGMLLAYSGVQSKKL TAMQRQLKKHFKDGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHV CDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291442
ORF Size	1449 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291442.1, NP_001278371.1
RefSeq Size	2376 bp
RefSeq ORF	1452 bp
Locus ID	216001
UniProt ID	Q8VCX5
MW	55.6 kDa
Gene Summary	Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (By similarity). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (By similarity). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.