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Sall4 (NM_175303) Mouse Tagged ORF Clone
CAT＃: MR227483
- TrueORF®
Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a
ORF Plasmid: tGFP
"NM_175303" in other vectors (4)
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CNY 10,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730441M18Rik; AA407717; AL022809; AW536104; C78083; C78563; C330011P20Rik; Tex20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227483 representing NM_175303
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGGCGCAAGCAGGCGAAGCCCCAGCACATCAACTGGGAGGAGGGCCAGGGCGAGCAGCCTCAGC AGCTACCGAGCCCCGACCTCGCCGAGGCGCTGGCGGCGGAGGAACCCGGTGCTCCAGTGAACTCCCCTGG GAACTGCGATGAAGCCTCAGAGGACTCCATACCGGTGAAGCGGCCCCGGCGGGAGGACACTCACATCTGC AACAAATGCTGTGCCGAGTTCTTTAGTCTCTCTGAATTCATGGAACACAAGAAAAGTTGCACTAAAACCC CTCCTGTCCTCATCATGAATGACAGCGAGGGGCCAGTGCCTTCAGAGGACTTTTCCAGAGCTGCCCTGAG CCACCAGCTGGGCAGCCCAAGCAATAAAGACAGTCTCCAGGAGAACGGCAGCAGCTCGGGGGACTTGAAG AAGCTGGGCACGGACTCCATCCTGTACTTGAAGACAGAGGCTACCCAGCCATCCACACCCCAGGACATAA GCTATTTACCCAAAGGCAAAGTAGCCAACACCAATGTGACTCTGCAGGCGCTCCGCGGCACCAAGGTGGC CGTGAACCAACGGGGTGCAGAGGCACCCATGGCGCCCATGCCTGCTGCCCAAGGCATCCCTTGGGTCCTG GAGCAGATCCTGTGCCTGCAGCAGCAGCAACTCCAGCAAATCCAGCTTACGGAACAGATTCGCGTCCAGG TGAACATGTGGGCAGCGCACGCGCTCCACTCTGGAGTGGCGGGGGCCGACACGCTGAAGGCCTTAAGCAG CCATGTGTCTCAGCAAGTGTCCGTGTCCCAGCAGGTGTCGGCTGCCGTGGCCCTGCTCAGCCAGAAAGCC TCAAACCCAGCTCTGTCGCTCGATGCCTTGAAACAAGCCAAGCTACCTCATGCCAGCGTCCCCTCCGCAG CCAGCCCGTTGTCCTCGGGGTTAACGTCCTTCACCTTGAAGCCTGACGGGACACGGGTTCTCCCCAACTT CGTGTCTCGCCTTCCCAGTGCCCTGCTACCTCAGACTCCGGGCTCTGTGCTCCTGCAGAGTCCCTTCTCC GCTGTGACGCTCGACCAGTCCAAGAAAGGAAAGGGGAAACCCCAGAACCTCTCCGCCTCTGCCTCGGTGT TAGATGTCAAGGCCAAGGACGAAGTCGTCCTCGGTAAGCACAAGTGTAGGTACTGTCCCAAGGTTTTCGG GACAGATAGCTCCCTTCAGATTCACCTTCGCTCCCACACCGGAGAGAGACCTTACGTGTGCCCTATCTGT GGTCACCGCTTCACCACCAAGGGCAATCTCAAGGTCCACTTACAACGACACCCTGAGGTGAAGGCAAACC CCCAGCTGTTGGCCGAATTCCAGGACAAAGGGGCAGTGAGTGCCGCTTCTCACTATGCACTCCCTGTCCC CGTCCCTGCCGATGAATCGAGTCTCTCTGTAGACGCCGAGCCTGTCCCGGTCACGGGAACCCCTTCTCTA GGGCTACCTCAAAAGCTCACGTCAGGGCCTAATTCCAGGGACCTCATGGGTGGCTCCTTGCCCAATGACA TGCAGCCAGGGCCTTCTCCAGAAAGTGAGGCGGGCCTTCCACTCCTTGGGGTGGGGATGATACATAATCC CCCAAAGGCTGGGGGCTTCCAGGGCACTGGGGCCCCAGAGTCAGGGTCCGAGACCCTGAAATTGCAGCAA CTAGTGGAGAACATAGACAAGGCCACTACTGACCCCAACGAGTGTCTCATTTGTCATCGGGTCCTCAGCT GTCAGAGTTCCCTGAAGATGCATTACCGTACCCACACAGGGGAGAGACCATTCCAGTGCAAGATCTGTGG CCGGGCCTTCTCCACCAAAGGCAACCTGAAGACACACCTTGGGGTTCACCGAACCAACACGACCGTAAAG ACCCAACATTCGTGCCCCATCTGCCAGAAGAAATTCACCAACGCCGTCATGTTACAGCAGCATATCCGGA TGCACATGGGTGGCCAGATCCCCAACACCCCTCTGCCAGAGAGTCCCTGTGACTTCACGGCTCCCGAGCC CGTGGCCGTCAGTGAGAATGGCAGTGCCAGCGGGGTCTGCCAGGACGACGCAGCAGAAGGGATGGAAGCC GAGGAGGTCTGTTCTCAGGATGTTCCCAGTGGCCCCTCAACTGTCTCTCTGCCGGTTCCCAGTGCCCACC TGGCATCGCCCTCTCTGGGCTTCTCTGTGTTGGCCTCCCTGGATACGCAGGGGAAAGGGGCTCTTCCGGC GCTGGCCCTGCAGAGGCAGAGCAGTCGAGAAAACAGCTCCCTGGAGGGCGGTGACACTGGTCCAGCCAAT GACTCTTCCTTGCTCGTGGGTGACCAGGAGTGTCAGAGCCGAAGCCCAGATGCCACGGAGACCATGTGCT ACCAGGCAGTGTCACCTGCCAATAGCCAAGCCGGAAGTGTCAAGTCCCGGTCTCCCGAGGGTCACAAGGC CGAGGGCGTGGAGAGCTGCCGCGTTGACACCGAAGGTCGTACCAGCCTCCCTCCAACATTTATCCGAGCA CAGCCCACCTTTGTCAAAGTTGAAGTGCCTGGCACCTTTGTGGGACCCCCCAGCATGCCCTCGGGTATGC CGCCTTTGCTAGCATCGCAGCCGCAGCCACGCCGCCAGGCCAAGCAGCACTGCTGCACACGGTGTGGAAA GAACTTCTCGTCTGCCAGTGCCCTGCAGATCCACGAGCGAACACACACGGGAGAGAAGCCTTTCGTGTGT AACATATGCGGGCGGGCCTTCACCACGAAAGGCAACCTGAAGGTACACTACATGACTCATGGGGCCAACA ATAACTCCGCCCGCCGGGGAAGGAAGCTGGCCATAGAGAACCCCATGGCCGCGCTGAGTGCTGAGGGAAA GAGAGCGCCCGAGGTGTTTTCCAAGGAGCTCCTGTCCCCCGCGGTGAGTGTGGACCCCGCCTCCTGGAAC CAGTACACCAGCGTCCTGAATGGGGGTCTGGCCATGAAGACCAACGAGATCTCCGTGATCCAGAGCGGAG GCATCCCCACGCTGCCTGTGTCGCTGGGGGCCAGCTCTGTGGTGAGCAATGGCACGATTTCCAAGCTTGA CGGCTCTCAGACCGGTGTGAGCATGCCCATGAGCGGGAACGGAGAAAAGCTCGCTGTTCCCGACGGCATG GCCAAACACCAGTTCCCTCACTTCCTGGAGGAAAATAAGATTGCTGTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227483 representing NM_175303
Red=Cloning site Green=Tags(s) MSRRKQAKPQHINWEEGQGEQPQQLPSPDLAEALAAEEPGAPVNSPGNCDEASEDSIPVKRPRREDTHIC NKCCAEFFSLSEFMEHKKSCTKTPPVLIMNDSEGPVPSEDFSRAALSHQLGSPSNKDSLQENGSSSGDLK KLGTDSILYLKTEATQPSTPQDISYLPKGKVANTNVTLQALRGTKVAVNQRGAEAPMAPMPAAQGIPWVL EQILCLQQQQLQQIQLTEQIRVQVNMWAAHALHSGVAGADTLKALSSHVSQQVSVSQQVSAAVALLSQKA SNPALSLDALKQAKLPHASVPSAASPLSSGLTSFTLKPDGTRVLPNFVSRLPSALLPQTPGSVLLQSPFS AVTLDQSKKGKGKPQNLSASASVLDVKAKDEVVLGKHKCRYCPKVFGTDSSLQIHLRSHTGERPYVCPIC GHRFTTKGNLKVHLQRHPEVKANPQLLAEFQDKGAVSAASHYALPVPVPADESSLSVDAEPVPVTGTPSL GLPQKLTSGPNSRDLMGGSLPNDMQPGPSPESEAGLPLLGVGMIHNPPKAGGFQGTGAPESGSETLKLQQ LVENIDKATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTTVK TQHSCPICQKKFTNAVMLQQHIRMHMGGQIPNTPLPESPCDFTAPEPVAVSENGSASGVCQDDAAEGMEA EEVCSQDVPSGPSTVSLPVPSAHLASPSLGFSVLASLDTQGKGALPALALQRQSSRENSSLEGGDTGPAN DSSLLVGDQECQSRSPDATETMCYQAVSPANSQAGSVKSRSPEGHKAEGVESCRVDTEGRTSLPPTFIRA QPTFVKVEVPGTFVGPPSMPSGMPPLLASQPQPRRQAKQHCCTRCGKNFSSASALQIHERTHTGEKPFVC NICGRAFTTKGNLKVHYMTHGANNNSARRGRKLAIENPMAALSAEGKRAPEVFSKELLSPAVSVDPASWN QYTSVLNGGLAMKTNEISVIQSGGIPTLPVSLGASSVVSNGTISKLDGSQTGVSMPMSGNGEKLAVPDGM AKHQFPHFLEENKIAVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175303 |
| ORF Size | 3201 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175303.4, NP_780512.2 |
| RefSeq Size | 5073 bp |
| RefSeq ORF | 3204 bp |
| Locus ID | 99377 |
| UniProt ID | Q8BX22 |
| MW | 113.6 kDa |
| Gene Summary | This gene belongs to the spalt family of zinc finger transcription factors. In mouse, functions for this gene have been described in many embryonic developmental processes, including brain, heart, and limb development. In addition, this gene is an important pluripotency factor that is required for stem cell maintenance. Homozygous mutant mice display embryonic lethality, while conditional knock-out in embryonic germ cells results in failure to establish a robust stem cell population. A pseudogene of this gene is found on chromosome 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223424 | Sall4 (untagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a, (10ug) |
CNY 13,680.00 |
|
| MG227483 | Sall4 (tGFP-tagged) - Mouse sal-like 4 (Drosophila) (Sall4) transcript variant a, (10ug) |
CNY 11,690.00 |
|
| MR227483L3 | Lenti ORF clone of Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a |
CNY 12,540.00 |
|
| MR227483L4 | Lenti ORF clone of Sall4 (mGFP-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a |
CNY 12,540.00 |
