Tet2 (NM_001040400) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ayu17-449; E130014J05Rik; mKIAA1546
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227061 representing NM_001040400 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAGGACAGAACCACCCATGCTGAGGGCACCAGACTGAGTCCATTCCTGATAGCACCACCTTCTC CCATCAGCCATACAGAACCTCTGGCTGTCAAACTCCAGAATGGAAGCCCGTTAGCAGAGAGACCTCATCC AGAAGTAAATGGAGACACCAAGTGGCAATCTTCCCAAAGCTGTTATGGAATATCCCACATGAAAGGCAGC CAGAGCAGTCATGAGAGTCCACATGAAGACAGAGGGTATTCCAGGTGCTTACAGAATGGAGGGATAAAAC GCACAGTCAGTGAACCATCTCTCTCTGGGCTCCATCCAAACAAGATATTGAAACTAGACCAAAAAGCTAA GGGAGAAAGCAATATCTTCGAGGAAAGCCAAGAAAGAAACCACGGTAAAAGCAGCCGTCAGCCAAATGTC TCCGGACTAAGTGATAATGGAGAGCCCGTGACCTCGACCACCCAGGAAAGTTCAGGTGCAGATGCTTTCC CAACACGGAACTACAATGGAGTTGAAATTCAGGTTCTCAACGAGCAGGAAGGGGAAAAAGGCAGGAGCGT TACATTACTTAAAAACAAGATTGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCCATTCTGAGGAGAAC ACACGTGGTGAACTCCTGGAAAAAACACAGTGTTATCCAGATTGTGTCTCCATTGCCGTGCAGAGTACCG CATCTCATGTAAACACCCCTAGCAGTCAGGCTGCTATCGAGTTGTCCCACGAGATCCCTCAACCATCGCT TACCTCAGCGCAGATCAATTTCTCCCAGACCTCAAGCTTGCAGCTGCCTCCAGAGCCAGCTGCAATGGTG ACTAAGGCCTGTGATGCTGATAATGCCAGTAAACCAGCTATAGTACCAGGTACCTGTCCTTTTCAGAAAG CAGAACACCAACAAAAGTCAGCTTTGGACATAGGCCCATCTCGTGCAGAAAACAAAACCATCCAAGGAAG CATGGAGCTATTTGCTGAAGAATACTATCCTAGTTCCGACCGGAATTTGCAAGCTTCGCATGGCAGCTCT GAACAGTATTCAAAGCAAAAGGAAACCAATGGTGCTTACTTCAGGCAAAGCTCGAAGTTCCCTAAAGATT CCATCTCTCCCACTACTGTGACCCCACCGTCACAATCACTTCTTGCTCCCCGTCTTGTTCTTCAGCCTCC TTTAGAAGGAAAAGGCGCTCTAAATGATGTAGCTTTGGAAGAACACCATGACTACCCCAACCGAAGCAAC CGAACTCTTTTAAGGGAAGGGAAAATAGACCATCAACCCAAGACATCATCTAGCCAGAGTCTGAATCCAT CTGTACATACACCCAACCCCCCCTTGATGCTTCCAGAACAGCATCAGAATGATTGTGGCTCACCGAGCCC TGAAAAGTCAAGAAAAATGTCAGAATATCTCATGTATTACCTGCCAAATCATGGCCACAGTGGAGGTTTA CAAGAACATAGCCAATACCTGATGGGGCACAGGGAGCAAGAGATTCCGAAGGATGCAAACGGGAAACAAA CGCAAGGCTCTGTACAGGCAGCACCTGGCTGGATAGAACTGAAAGCCCCGAATTTGCATGAAGCACTCCA TCAGACAAAACGCAAGGATATATCCTTGCACTCAGTCCTCCACTCTCAGACCGGCCCTGTCAATCAGATG AGCTCCAAACAGTCCACTGGCAATGTCAACATGCCAGGAGGATTCCAAAGGCTACCTTACCTCCAGAAAA CAGCCCAGCCAGAGCAGAAGGCACAAATGTACCAAGTGCAAGTGAACCAAGGACCGTCTCCAGGTATGGG GGACCAACATCTTCAGTTCCAGAAAGCTTTATACCAGGAGTGCATCCCCAGGACAGATCCGTCATCTGAG GCTCACCCGCAAGCACCGAGCGTTCCTCAGTATCATTTCCAGCAAAGAGTAAATCCCTCCAGTGATAAGC ATTTGAGTCAACAGGCCACAGAGACTCAACGGTTATCAGGCTTTTTACAACATACTCCTCAGACGCAGGC ATCACAAACACCAGCATCCCAGAACTCAAATTTCCCTCAAATCTGCCAGCAGCAGCAGCAGCAGCAGTTA CAGAGGAAGAATAAAGAGCAAATGCCTCAGACTTTCTCTCATCTCCAAGGTAGCAATGATAAGCAAAGAG AAGGCTCGTGCTTTGGCCAGATTAAAGTGGAAGAAAGCTTTTGTGTCGGAAATCAGTACTCCAAATCAAG TAATTTCCAAACTCACAATAATACCCAAGGGGGGTTGGAGCAAGTACAAAATATAAATAAAAATTTTCCT TATTCGAAGATCTTAACACCAAATTCGAGCAACTTACAGATTCTCCCTTCAAATGACACACACCCGGCTT GTGAGCGGGAACAAGCTCTACATCCCGTAGGAAGTAAGACCTCAAACCTGCAGAACATGCAGTATTTCCC GAATAATGTGACCCCAAATCAGGACGTTCACCGGTGCTTTCAGGAACAAGCGCAGAAGCCTCAGCAAGCT TCGTCTCTACAGGGGCTTAAGGACAGAAGCCAGGGTGAGTCTCCAGCCCCACCAGCTGAGGCAGCTCAAC AGAGGTATTTGGTGCATAATGAAGCAAAGGCACTCCCTGTGCCTGAGCAAGGAGGAAGTCAGACACAGAC CCCTCCTCAGAAGGACACTCAGAAGCACGCTGCCTTAAGGTGGCTTCTCTTACAGAAGCAAGAACAGCAG CAAACACAGCAATCCCAGCCTGGTCATAACCAGATGCTTAGGCCAATCAAGACTGAGCCTGTATCCAAAC CTTCTTCCTATAGATACCCCTTGTCACCGCCACAAGAAAATATGTCCAGCAGGATAAAGCAAGAGATCTC CTCTCCAAGCCGTGACAATGGGCAGCCAAAGAGCATCATTGAGACCATGGAACAGCACCTGAAGCAGTTT CAGCTCAAGTCACTCTGTGACTATAAGGCTCTGACTCTCAAGTCACAGAAACACGTGAAAGTGCCAACAG ATATCCAGGCTGCAGAATCGGAGAACCACGCCCGAGCTGCAGAGCCTCAAGCAACCAAAAGCACAGATTG TTCTGTTCTCGACGATGTTTCAGAATCAGATACTCCTGGTGAACAAAGTCAGAATGGCAAATGTGAAGGA TGCAATCCAGACAAAGATGAAGCTCCTTATTATACCCATCTAGGAGCTGGTCCTGATGTGGCAGCTATTA GAACACTCATGGAAGAAAGGTATGGAGAGAAGGGTAAAGCTATTAGGATTGAAAAAGTCATATATACTGG TAAAGAAGGCAAGAGCTCTCAGGGATGTCCTATTGCTAAATGGGTATATCGGAGATCGAGTGAGGAGGAG AAACTACTGTGTTTGGTACGAGTGCGACCTAATCACACATGTGAGACGGCGGTGATGGTAATTGCCATCA TGTTGTGGGACGGAATCCCAAAGCTACTCGCATCAGAACTCTACTCAGAACTTACAGATATCTTGGGCAA GTGTGGCATATGCACCAACCGTCGCTGTTCTCAGAATGAAACTAGAAACTGTTGTTGTCAGGGTGAGAAT CCAGAGACCTGTGGTGCCTCCTTTTCTTTTGGTTGTTCTTGGAGCATGTACTATAATGGATGTAAGTTTG CCAGAAGCAAGAAACCAAGGAAATTTAGGCTACATGGAGCTGAGCCAAAAGAGGAAGAGAGACTAGGTTC TCATTTGCAAAACCTGGCTACTGTCATTGCTCCAATATACAAGAAGCTTGCACCCGATGCATACAATAAT CAGGTTGAATTTGAACACCAAGCCCCAGACTGCTGTTTGGGTCTGAAGGAAGGCCGGCCATTCTCAGGAG TCACTGCATGTTTGGACTTCTCTGCTCATTCCCACAGAGACCAGCAGAACATGCCAAATGGCAGTACAGT GGTGGTCACCCTCAATAGAGAAGACAATCGAGAAGTCGGAGCTAAGCCTGAGGATGAGCAGTTCCACGTG CTGCCTATGTACATCATCGCCCCTGAGGATGAGTTTGGGAGTACGGAAGGCCAGGAGAAGAAGATACGGA TGGGGTCCATTGAGGTTCTGCAGTCATTTCGGAGGAGAAGGGTCATAAGGATAGGAGAGCTGCCCAAGAG TTGCAAGAAGAAAGCGGAGCCCAAGAAAGCCAAGACCAAGAAAGCAGCTCGAAAGCGTTCCTCTCTGGAG AACTGCTCCAGTAGGACTGAGAAGGGAAAGTCTTCCTCACATACAAAGCTGATGGAAAATGCAAGCCATA TGAAACAAATGACAGCACAACCGCAGCTTTCGGGCCCGGTCATCCGGCAGCCACCAACACTCCAGAGGCA CCTTCAGCAAGGGCAGAGGCCACAGCAGCCGCAGCCACCTCAGCCGCAGCCGCAGACGACACCTCAGCCA CAGCCACAGCCACAGCATATCATGCCCGGTAACTCTCAGTCTGTTGGTTCTCATTGTTCTGGATCCACCA GTGTCTACACGAGACAGCCTACTCCTCACAGTCCTTATCCCAGCTCAGCACACACCTCAGATATTTATGG AGATACCAACCATGTGAACTTTTACCCCACTTCATCTCATGCCTCGGGTTCATATTTGAATCCTTCTAAT TACATGAACCCCTACCTTGGGCTTTTGAATCAGAATAACCAATATGCACCTTTTCCATACAATGGGAGTG TGCCAGTGGACAATGGTTCCCCTTTCTTAGGTTCTTATTCCCCCCAGGCTCAGTCCAGGGATCTACATAG ATATCCAAACCAGGACCATCTCACCAATCAGAACTTACCACCCATCCACACCCTTCACCAACAGACGTTT GGGGACAGTCCCTCTAAGTACTTAAGTTATGGAAACCAAAATATGCAGAGAGATGCCTTCACTACTAACT CCACCCTAAAACCAAATGTACACCACCTAGCAACGTTTTCTCCTTACCCCACCCCCAAGATGGATAGTCA TTTCATGGGAGCTGCCTCCAGATCACCATACAGCCACCCACACACTGACTACAAAACCAGTGAGCATCAT CTACCCTCTCACACGATCTACAGCTACACGGCAGCAGCTTCGGGGAGCAGTTCCAGCCACGCCTTCCACA ACAAGGAGAATGACAACATAGCCAATGGGCTCTCAAGAGTGCTTCCAGGGTTTAATCATGATAGAACTGC TTCTGCCCAAGAACTATTATACAGTCTGACTGGCAGCAGTCAGGAGAAGCAGCCTGAGGTGTCAGGCCAG GATGCAGCTGCTGTGCAGGAAATTGAGTATTGGTCAGATAGTGAGCACAACTTTCAGGATCCTTGCATTG GAGGGGTGGCTATAGCTCCAACTCATGGGTCAATTCTTATTGAGTGTGCAAAGTGTGAGGTTCATGCCAC AACCAAAGTAAACGATCCCGACCGGAATCACCCCACCAGGATCTCACTTGTACTGTATAGGCATAAGAAT TTGTTTCTACCAAAACATTGTTTGGCTCTCTGGGAAGCCAAAATGGCTGAAAAGGCCCGGAAAGAGGAAG AGTGCGGAAAGAATGGATCAGACCACGTGTCTCAGAAAAATCATGGCAAACAGGAAAAGCGTGAGCCCAC AGGGCCACAGGAACCCAGTTACCTGCGTTTCATCCAGTCTCTTGCTGAGAACACAGGGTCTGTGACTACG GATTCTACCGTGACTACATCACCATATGCTTTCACTCAGGTCACAGGGCCTTACAACACATTTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227061 representing NM_001040400 Red=Cloning site Green=Tags(s) MEQDRTTHAEGTRLSPFLIAPPSPISHTEPLAVKLQNGSPLAERPHPEVNGDTKWQSSQSCYGISHMKGS QSSHESPHEDRGYSRCLQNGGIKRTVSEPSLSGLHPNKILKLDQKAKGESNIFEESQERNHGKSSRQPNV SGLSDNGEPVTSTTQESSGADAFPTRNYNGVEIQVLNEQEGEKGRSVTLLKNKIVLMPNGATVSAHSEEN TRGELLEKTQCYPDCVSIAVQSTASHVNTPSSQAAIELSHEIPQPSLTSAQINFSQTSSLQLPPEPAAMV TKACDADNASKPAIVPGTCPFQKAEHQQKSALDIGPSRAENKTIQGSMELFAEEYYPSSDRNLQASHGSS EQYSKQKETNGAYFRQSSKFPKDSISPTTVTPPSQSLLAPRLVLQPPLEGKGALNDVALEEHHDYPNRSN RTLLREGKIDHQPKTSSSQSLNPSVHTPNPPLMLPEQHQNDCGSPSPEKSRKMSEYLMYYLPNHGHSGGL QEHSQYLMGHREQEIPKDANGKQTQGSVQAAPGWIELKAPNLHEALHQTKRKDISLHSVLHSQTGPVNQM SSKQSTGNVNMPGGFQRLPYLQKTAQPEQKAQMYQVQVNQGPSPGMGDQHLQFQKALYQECIPRTDPSSE AHPQAPSVPQYHFQQRVNPSSDKHLSQQATETQRLSGFLQHTPQTQASQTPASQNSNFPQICQQQQQQQL QRKNKEQMPQTFSHLQGSNDKQREGSCFGQIKVEESFCVGNQYSKSSNFQTHNNTQGGLEQVQNINKNFP YSKILTPNSSNLQILPSNDTHPACEREQALHPVGSKTSNLQNMQYFPNNVTPNQDVHRCFQEQAQKPQQA SSLQGLKDRSQGESPAPPAEAAQQRYLVHNEAKALPVPEQGGSQTQTPPQKDTQKHAALRWLLLQKQEQQ QTQQSQPGHNQMLRPIKTEPVSKPSSYRYPLSPPQENMSSRIKQEISSPSRDNGQPKSIIETMEQHLKQF QLKSLCDYKALTLKSQKHVKVPTDIQAAESENHARAAEPQATKSTDCSVLDDVSESDTPGEQSQNGKCEG CNPDKDEAPYYTHLGAGPDVAAIRTLMEERYGEKGKAIRIEKVIYTGKEGKSSQGCPIAKWVYRRSSEEE KLLCLVRVRPNHTCETAVMVIAIMLWDGIPKLLASELYSELTDILGKCGICTNRRCSQNETRNCCCQGEN PETCGASFSFGCSWSMYYNGCKFARSKKPRKFRLHGAEPKEEERLGSHLQNLATVIAPIYKKLAPDAYNN QVEFEHQAPDCCLGLKEGRPFSGVTACLDFSAHSHRDQQNMPNGSTVVVTLNREDNREVGAKPEDEQFHV LPMYIIAPEDEFGSTEGQEKKIRMGSIEVLQSFRRRRVIRIGELPKSCKKKAEPKKAKTKKAARKRSSLE NCSSRTEKGKSSSHTKLMENASHMKQMTAQPQLSGPVIRQPPTLQRHLQQGQRPQQPQPPQPQPQTTPQP QPQPQHIMPGNSQSVGSHCSGSTSVYTRQPTPHSPYPSSAHTSDIYGDTNHVNFYPTSSHASGSYLNPSN YMNPYLGLLNQNNQYAPFPYNGSVPVDNGSPFLGSYSPQAQSRDLHRYPNQDHLTNQNLPPIHTLHQQTF GDSPSKYLSYGNQNMQRDAFTTNSTLKPNVHHLATFSPYPTPKMDSHFMGAASRSPYSHPHTDYKTSEHH LPSHTIYSYTAAASGSSSSHAFHNKENDNIANGLSRVLPGFNHDRTASAQELLYSLTGSSQEKQPEVSGQ DAAAVQEIEYWSDSEHNFQDPCIGGVAIAPTHGSILIECAKCEVHATTKVNDPDRNHPTRISLVLYRHKN LFLPKHCLALWEAKMAEKARKEEECGKNGSDHVSQKNHGKQEKREPTGPQEPSYLRFIQSLAENTGSVTT DSTVTTSPYAFTQVTGPYNTFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001040400
ORF Size	5736 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001040400.2, NP_001035490.2
RefSeq Size	9199 bp
RefSeq ORF	5739 bp
Locus ID	214133
UniProt ID	Q4JK59
MW	212.6 kDa
Gene Summary	Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.[UniProtKB/Swiss-Prot Function]

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