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Pecam1 (NM_008816) Mouse Tagged ORF Clone
CAT＃: MR227044
- TrueORF®
Pecam1 (Myc-DDK-tagged) - Mouse platelet/endothelial cell adhesion molecule 1 (Pecam1), transcript variant 1
ORF Plasmid: tGFP
"NM_008816" in other vectors (3)
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CNY 5,248.00
CNY 5,800.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C85791; Cd31; Pecam; PECAM-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227044 representing NM_008816
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTGGCTCTGGGACTCACGCTGGTGCTCTATGCAAGCCTCCAGGCTGAGGAAAACTCCTTCACCA TCAACAGCATCCATATGGAAAGCCTGCCATCATGGGAGGTGATGAATGGGCAGCAACTGACCCTGGAGTG CCTTGTGGACATCAGCACCACCTCGAAAAGCAGGTCTCAGCACCGGGTGCTGTTCTATAAGGACGATGCG ATGGTGTATAACGTCACCTCCAGGGAGCACACCGAGAGCTACGTCATTCCTCAGGCTCGGGTCTTCCACT CCGGGAAGTACAAATGCACAGTGATGCTGAACAACAAGGAAAAAACCACGATTGAGTACGAGGTGAAGGT GCATGGCGTATCCAAGCCCAAGGTGACACTGGACAAAAAGGAGGTGACAGAAGGCGGGGTCGTGACGGTC AATTGTTCCTTGCAAGAAGAAAAGCCACCGATCTTTTTTAAAATTGAAAAATTAGAAGTGGGGACAAAGT TTGTCAAGCGAAGGATAGATAAGACCTCCAACGAGAACTTTGTGCTCATGGAATTCCCCATTGAGGCGCA GGACCACGTGTTAGTGTTTCGCTGCCAAGCTGGGATCCTGTCCGGATTCAAATTGCAGGAGTCAGAACCC ATCAGGAGTGAATACGTCACCGTGCAGGAGTCCTTCTCCACTCCCAAGTTTGAAATCAAGCCCCCTGGGA TGATCATAGAAGGGGACCAGCTGCACATTAGGTGCATAGTTCAAGTGACACACTTGGTCCAGGAGTTTAC AGAAATTATCATCCAAAAAGACAAGGCGATTGTAGCCACCTCCAAGCAAAGCAGTGAAGCTGTCTACTCA GTCATGGCCATGGTCGAGTACAGTGGACACTACACCTGCAAAGTGGAATCAAACCGTATCTCCAAAGCCA GTAGCATCATGGTCAACATAACAGAGCTGTTTCCCAAGCCGAAGTTAGAGTTCTCCTCCAGTCGTCTGGA CCAAGGGGAGTTGTTGGACCTGTCCTGCTCCGTCTCGGGCACACCTGTAGCCAACTTCACCATCCAGAAG GAAGAGACGGTCTTGTCGCAGTATCAGAATTTCAGCAAGATCGCCGAGGAGAGTGACAGCGGGGAGTACA GCTGTACTGCAGGCATCGGCAAAGTGGTCAAGAGAAGCGGCCTGGTACCGATCCAGGTGTGCGAAATGCT CTCGAAGCCCAGTATTTTTCATGATGCCAAGTCTGAGATCATAAAAGGACATGCCATAGGCATCAGCTGC CAGTCCGAAAATGGAACTGCACCCATCACTTACCACCTTATGAAAGCAAAGAGTGACTTCCAGACTCTCG AGGTGACCTCCAATGACCCAGCAACATTCACAGATAAGCCCACCAGAGACATGGAATACCAGTGCAGAGC GGATAATTGCCATTCCCACCCCGCGGTGTTCAGCGAGATCCTGAGGGTCAGGGTGATAGCCCCAGTGGAT GAAGTTGTGATTTCCATCCTGTCGAGTAACGAGGTACAGTCTGGAAGTGAAATGGTACTTCGGTGCTCTG TGAAAGAGGGGACGAGCCCAATCACGTTTCAGTTTTACAAAGAAAAGGAGGACAGACCCTTCCACCAAGC GGTCGTGAATGACACCCAAGCGTTTTGGCACAACAAACAAGCTAGCAAGAAGCAGGAAGGACAGTACTAC TGTACAGCCTCCAACAGAGCCAGCAGTATGAGGACCAGTCCCCGAAGCAGCACTCTTGCAGTCAGAGTCT TCCTTGCCCCATGGAAGAAAGGGCTCATTGCGGTGGTTGTCATTGGAGTGGTCATCGCCACCTTAATAGT TGCAGCCAAATGCTACTTCCTGAGGAAAGCCAAGGCCAAACAGAAACCCGTGGAGATGTCCAGGCCAGCT GCTCCACTTCTGAACTCCAACAGCGAGAAGATTTCTGAGCCTAGTGTGGAAGCCAACAGCCATTACGGTT ATGATGATGTTTCTGGAAATGATGCAGTAAAACCCATAAATCAAAATAAAGACCCCCAGAACATGGATGT AGAATACACAGAAGTGGAAGTGTCCTCCCTTGAGCCTCACCAAGCTCTGGGAACGAGAGCCACAGAGACG GTGTACAGTGAGATCCGGAAGGTCGACCCTAAGAACGGAAGGCTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227044 representing NM_008816
Red=Cloning site Green=Tags(s) MLLALGLTLVLYASLQAEENSFTINSIHMESLPSWEVMNGQQLTLECLVDISTTSKSRSQHRVLFYKDDA MVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTV NCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEP IRSEYVTVQESFSTPKFEIKPPGMIIEGDQLHIRCIVQVTHLVQEFTEIIIQKDKAIVATSKQSSEAVYS VMAMVEYSGHYTCKVESNRISKASSIMVNITELFPKPKLEFSSSRLDQGELLDLSCSVSGTPVANFTIQK EETVLSQYQNFSKIAEESDSGEYSCTAGIGKVVKRSGLVPIQVCEMLSKPSIFHDAKSEIIKGHAIGISC QSENGTAPITYHLMKAKSDFQTLEVTSNDPATFTDKPTRDMEYQCRADNCHSHPAVFSEILRVRVIAPVD EVVISILSSNEVQSGSEMVLRCSVKEGTSPITFQFYKEKEDRPFHQAVVNDTQAFWHNKQASKKQEGQYY CTASNRASSMRTSPRSSTLAVRVFLAPWKKGLIAVVVIGVVIATLIVAAKCYFLRKAKAKQKPVEMSRPA APLLNSNSEKISEPSVEANSHYGYDDVSGNDAVKPINQNKDPQNMDVEYTEVEVSSLEPHQALGTRATET VYSEIRKVDPKNGRLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008816 |
| ORF Size | 2148 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008816.3 |
| RefSeq Size | 3305 bp |
| RefSeq ORF | 2151 bp |
| Locus ID | 18613 |
| UniProt ID | Q08481 |
| MW | 80.5 kDa |
| Gene Summary | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227044 | Pecam1 (tGFP-tagged) - Mouse platelet/endothelial cell adhesion molecule 1 (Pecam1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR227044L3 | Lenti ORF clone of Pecam1 (Myc-DDK-tagged) - Mouse platelet/endothelial cell adhesion molecule 1 (Pecam1), transcript variant 1 |
CNY 7,700.00 |
|
| MR227044L4 | Lenti ORF clone of Pecam1 (mGFP-tagged) - Mouse platelet/endothelial cell adhesion molecule 1 (Pecam1), transcript variant 1 |
CNY 7,648.00 |
