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Pnpla2 (NM_001163689) Mouse Tagged ORF Clone
CAT＃: MR226260
- TrueORF®
Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1
ORF Plasmid: tGFP
"NM_001163689" in other vectors (4)
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CNY 3,330.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226260 representing NM_001163689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGAGGGAGACCAAGTGGAACATCTCATTCGCTGGCTGCGGCTTCCTCGGGGTCTACCACATTG GCGTGGCCTCCTGCCTCCGTGAGCACGCGCCCTTCCTGGTGGCCAACGCCACTCACATCTACGGAGCCTC GGCAGGGGCGCTCACCGCCACAGCGCTGGTCACTGGGGCCTGCCTGGGTGAAGCAGGTGCCAACATTATT GAGGTGTCCAAGGAGGCCCGGAAGCGGTTCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCA TCCGTGGCTGTCTACTAAAGACCCTGCCTGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTC CCTGACTCGTGTTTCAGACGGAGAGAACGTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAG GCCAATGTCTGCAGCACATTTATCCCGGTGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCT ATGTGGATGGCGGCATTTCAGACAACTTGCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATT CTCAGGCGAGAGTGACATCTGCCCTCAGGACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACC AGCATCCAGTTCAACCTTCGCAATCTCTACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCC TCCGAGAGATGTGCAAACAGGGCTACAGAGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCA ACCCAACCCTTTGCTGGCACTGCCCCCAGTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTG GAGGAGAGGGCTGGAGAGGAGGATCAATTGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTG CCAGACTCAATGAGGCCCTGCTGGAGGCCTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACAT GCTACCAGTGCGCCTGGCAACGGCCATGATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTC ACCATCCGCTTGTTGGAGTGGCTGCCTGATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTA GCATCTGCCAGTATCTGGTGATGAGGGCCAAGAGGAAATTGGGTGGCCATCTGCCTTCCAGACTGTCTGA GCAGGTGGAACTGCGACGTGCCCAGTCTCTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCC CTACCCAACTGGGTACGAAACAACCTCTCACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTC AGCTACTGCTGGGTCTCTTCTGCACCAATGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGC CAGCCCCACTGCCGCAGATCCTGCCACCCCACAGGATCCACCTGGCCTCCCGCCTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226260 representing NM_001163689
Red=Cloning site Green=Tags(s) MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANII EVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQ ANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT SIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVV EERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSF TIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGGHLPSRLSEQVELRRAQSLPSVPLSCATYSEA LPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163689 |
| ORF Size | 1458 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163689.1, NP_001157161.1 |
| RefSeq Size | 2635 bp |
| RefSeq ORF | 1461 bp |
| Locus ID | 66853 |
| UniProt ID | Q8BJ56 |
| MW | 54.1 kDa |
| Gene Summary | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ATGL activity regulates GLUT1-mediated glucose uptake and lactate production via TXNIP stability in adipocytes
,Beg, M;Zhang, W;McCourt, AC;Enerbäck, S;,
The Journal of biological chemistry
,PubMed ID 33508319
[PNPLA2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216670 | Pnpla2 (untagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1, (10ug) |
CNY 6,270.00 |
|
| MG226260 | Pnpla2 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
| MR226260L3 | Lenti ORF clone of Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1 |
CNY 5,230.00 |
|
| MR226260L4 | Lenti ORF clone of Pnpla2 (mGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1 |
CNY 5,230.00 |
