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Smurf1 (NM_001038627) Mouse Tagged ORF Clone
CAT＃: MR226199
- TrueORF®
Smurf1 (Myc-DDK-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1
ORF Plasmid: tGFP
"NM_001038627" in other vectors (3)
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CNY 7,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930431E10Rik; mKIAA1625 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226199 representing NM_001038627
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTATTGTGTGCCAAGA ACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAGATTGTTGTGGACGGCTCTGGGCA GTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGAACCAGCACTATGACCTGTATGTT GGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGATCCACAAGAAGCAGGGGGCTGGCT TCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAAGACACTGGCTACCAGCGTTTGGA TCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAATAGTGGTCAGTTTACAGACCCGA GACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCTGGAGAACGAAGGAACAGTGTATG AAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCTGCCCCATATACAGATGGTACTGG TGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCCAAGATCAGAGACTCCTGGTACAG CGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAACCGACCACATGGCCACCAGTCGC CAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAAGTTTACTTTTTGCACACGCAGAC TGGAGTCAGTACATGGCATGACCCCAGGATCCCCAGAGACCTTAACAGTGTGAACTGCGATGAACTTGGG CCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAATCTATTTTGTAGATCACAATAATA GGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAG CCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGCTTCCTGCCCAGAGATATGAGAGG GACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCAGCAGCCCCAGGCTGGTCACTGTC GAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAGATCATGAAGATGCGGCCAAAAGA CCTAAAGAAGCGCCTGATGGTGAAGTTCCGAGGGGAGGAAGGTTTGGACTATGGTGGAGTGGCTCGGGAG TGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACTCTTCCAGTATTCCACGGACAATA TTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCATCTGTCATACTTCCACTTTGTGGG TCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGGGTTTCACAGTTCCGTTCTACAAG CAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGACCCAGAACTGCATAAGAGCTTGG TGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTCTGCGTGGAGCACAACGCTTTCGG GCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTGTCACTGAGGAGAACAAGAAGGAA TACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCTTAGCACTTCAGAAGG GGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAGAAGGAACTAGAGCTGATAATAGG TGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGCTGAAACACTGTGTGGCAGACAGC AACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGAGAGGAGAGCCAGACTCCTGCAGT TTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTGCAAGGCTCTACAGGCGCGGCAGG GCCCCGGCTGTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGGCCCATACCTGCTTT AATCGGATCGACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCTGACAGCGGTGGAGG AAACCTGTGGCTTTGCAGTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226199 representing NM_001038627
Red=Cloning site Green=Tags(s) MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYV GKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTR DRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQ RLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELG PLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYER DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVARE WLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYK QLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKE YVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADS NIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCF NRIDIPPYESYEKLYEKLLTAVEETCGFAVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001038627 |
| ORF Size | 2193 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001038627.1, NP_001033716.1 |
| RefSeq Size | 5333 bp |
| RefSeq ORF | 2196 bp |
| Locus ID | 75788 |
| UniProt ID | Q9CUN6 |
| MW | 83.8 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226199 | Smurf1 (tGFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1) transcript variant 1, (10ug) |
CNY 8,080.00 |
|
| MR226199L3 | Lenti ORF clone of Smurf1 (Myc-DDK-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1 |
CNY 9,220.00 |
|
| MR226199L4 | Lenti ORF clone of Smurf1 (mGFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1 |
CNY 9,220.00 |
