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Hcar1 (NM_175520) Mouse Tagged ORF Clone
CAT＃: MR225816
- TrueORF®
Gpr81 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 81 (Gpr81)
ORF Plasmid: tGFP
"NM_175520" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gpr81 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225816 representing NM_175520
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTCCTCTCTCCAACTGCTATGGACAACGGGTCGTGCTGTCTCATCGAGGGGGAGCCCATCTCCC AAGTGATGCCTCCTCTACTCATCCTGGTCTTCGTGCTTGGCGCCCTGGGCAACGGCATAGCCCTGTGCGG CTTCTGCTTTCACATGAAGACCTGGAAGTCAAGCACTATTTACCTTTTCAACTTGGCTGTGGCCGATTTT CTCCTCATGATCTGCTTACCCCTTCGGACAGACTACTACCTCAGACGCAGACACTGGATTTTTGGAGATA TCGCCTGTCGCCTGGTCCTCTTCAAGCTGGCCATGAATAGGGCCGGGAGCATTGTCTTCCTCACTGTGGT GGCTGTGGATAGGTATTTCAAAGTGGTCCACCCCCACCATATGGTGAATGCCATCTCCAACCGGACTGCC GCCGCCACCGCCTGTGTCCTCTGGACTTTGGTCATCTTGGGGACTGTGTATCTTCTGATGGAGAGTCACC TGTGTGTGCAGGGGACACTGTCGTCCTGTGAGAGCTTCATCATGGAGTCAGCCAACGGGTGGCACGATGT CATGTTCCAGCTGGAGTTCTTCCTGCCCCTGACAATCATCTTGTTCTGCTCGGTCAACGTTGTTTGGAGC CTGAGACGGAGGCAGCAGCTGACCAGACAGGCTCGGATGAGGAGGGCCACCCGGTTCATCATGGTGGTGG CTTCTGTGTTCATCACGTGTTACCTGCCCAGCGTGCTGGCTAGGCTCTACTTCCTCTGGACGGTGCCCAC TAGTGCCTGTGACCCCTCTGTCCACACAGCCCTCCACGTCACCCTGAGCTTCACCTACCTGAACAGTATG CTGGATCCCCTTGTATATTACTTCTCAAGCCCCTCGCTCCCCAAATTCTACACCAAGCTCACAATCTGCA GCCTGAAGCCCAAACGCCCAGGACGCACGAAGACGCGGAGGTCAGAAGAGATGCCAATTTCGAACCTCTG CAGTAAGAGCTCCATCGATGGGGCAAATCGTTCCCAGAGGCCATCTGACGGGCAGTGGGATCTCCAAGTG TGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225816 representing NM_175520
Red=Cloning site Green=Tags(s) MPVLSPTAMDNGSCCLIEGEPISQVMPPLLILVFVLGALGNGIALCGFCFHMKTWKSSTIYLFNLAVADF LLMICLPLRTDYYLRRRHWIFGDIACRLVLFKLAMNRAGSIVFLTVVAVDRYFKVVHPHHMVNAISNRTA AATACVLWTLVILGTVYLLMESHLCVQGTLSSCESFIMESANGWHDVMFQLEFFLPLTIILFCSVNVVWS LRRRQQLTRQARMRRATRFIMVVASVFITCYLPSVLARLYFLWTVPTSACDPSVHTALHVTLSFTYLNSM LDPLVYYFSSPSLPKFYTKLTICSLKPKRPGRTKTRRSEEMPISNLCSKSSIDGANRSQRPSDGQWDLQV C TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175520 |
| ORF Size | 1053 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175520.4, NP_780729.2 |
| RefSeq Size | 3285 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 243270 |
| UniProt ID | Q8C131 |
| MW | 40.2 kDa |
| Gene Summary | Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC213165 | Gpr81 (untagged) - Mouse G protein-coupled receptor 81 (Gpr81), (10ug) |
CNY 5,856.00 |
|
| MG225816 | Gpr81 (tGFP-tagged) - Mouse G protein-coupled receptor 81 (Gpr81), (10ug) |
CNY 7,088.00 |
|
| MR225816L3 | Lenti ORF clone of Gpr81 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 81 (Gpr81) |
CNY 5,890.00 |
|
| MR225816L4 | Lenti ORF clone of Gpr81 (mGFP-tagged) - Mouse G protein-coupled receptor 81 (Gpr81) |
CNY 7,888.00 |
