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Pnpla8 (NM_026164) Mouse Tagged ORF Clone
CAT＃: MR225706
- TrueORF®
Pnpla8 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 8 (Pnpla8)
ORF Plasmid: tGFP
"NM_026164" in other vectors (4)
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CNY 5,688.00
CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200006O19Rik; AI467579; Ipla2(gamma) |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225706 representing NM_026164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATTAATTTGACTCTAGATATATATATTTACTTCCTTAATAATGCAAGAAGTCTTTGTGGGAAAC AGAGAAGCAAACAATTGCATTTTGTGTGTTCTAAACAGTATTGGCGCATGAACCATGTCAATGTGCACAG AGAGTTTCACACAAGTAAGAAAAGCTGTAAATGGAATAGAAGTGAAGCACATTGCAGCAAACACTGGCAC TCTCCAAGCAACCATGGCTTACACTTTGGAATTGTGAGACTTAGCACTTCTGCTCCCAAGGGACTTACAA AAGTGAGCATTCATATGTCCCGTATTAAAAGTACTTTGAATTCAGTTTCAAAGGCTATTTTTGGCAGTCA AAATGAAATGGTTACACGGTTAGCTCAATTTAAGCCAAGTTCTCGAATATTAAGAAAAGTATCAGATAAG GGATGGTTAAAACAAAAAAATGTTAAGCAAGCCGTCGAGTCTCTGAAAAACTATAGTGATAAATCAGCAG GAAAGAATTCTTTAGCAGAACAGAAAAGTTACTTTGCAGATAAAGAAGAAGATTCAGGTAAACACAGCCT TTTCCATTACACATACGGTATAACCACCAGATTTGGAGAGTCATTCTCCGTTTTAGCAAATCATATTAAT TCCTATTTCAAAAGCAAGGGAAAAATGTCTCAAACAAAGGAAGATAAACAGCTTCAGGACAAGCCAGACC TTGAAGAAAGGAAATCCAGTTCTCCAGGCCCTGACACCGTGGCAGACAGGCCAGACTCGGAATCTCCCCT AGAAGTTAAAGACAAACTTTCCAGCCCGACCCAGATGCCTGAGGCTCACCCAGTGTCTGCAAAACAAAGT ATTGCTAACTTCCTTTCTCGTCCCACTGAAGGCGTACAAGCCTTAGTCGGTGGTTACATTGGTGGACTTG TACCCAAATTAAAGTCTGATCCCAAGAGTCCGCCCGAAGAACAGGAAGTGTCTGCTAAAACAGAGCAGGC TGTTAACAAAGACAAGAAGGCAGAGGAGAAAAAGCGTGTGCTACTTCAGCAAGAAAAGATTATTGCAAGA GTGAGTATTGATAACAGAACTCGAGCCTTAGTTCAGGCCTTAAGAAGAACAGCCGACCCAAAGCTCTGTA TTACTAGGGTTGAAGAACTGACTTTTCATCTTCTAGAATTTCCTGAAGGAAAAGGAGTGGCTATCAAGGA AAAAATTATTCCATATTTATTACGGCTGAGACAAGTTAAGGATGAAACTCTTCAGGCTGCAGTTAGAGAA ATTTTGGCTTTAATTGGTTATGTAGATCCAGTGAAAGGCAGAGGAATCCGAATCCTCACCATTGATGGTG GAGGAACAAGAGGTGTGGTTGCTCTCCAGACTCTGAGGAAGTTGGTTGAACTTACTCAGAAGCCGATTCA TCAGCTTTTTGATTACATCTGTGGAGTAAGCACAGGGGCCATATTAGCATTTATGCTGGGATTGTTTCAC ATGCCGCTGGATGAATGTGAGGAACTCTATCGAAAGTTGGGCTCAGATGTGTTCACACAGAATGTCATTG TCGGGACCGTCAAAATGAGTTGGAGCCATGCGTTTTATGACAGTAACACGTGGGAAAAGATCCTCAAGGA TAGAATTGGATCTGCACTAATGATTGAAACAGCGAGAAACCCCGCATGTCCTAAGGTAGCTGCTATAAGT ACCATAGTAAACAGAGGACAAACACCAAAAGCTTTTGTGTTCAGAAACTATGGTCATTTTCCTGGCACCA ACTCTCACTATTTAGGAGGCTGTCAGTATAAAATGTGGCAGGCCATTAGAGCCTCATCAGCTGCTCCAGG CTACTTTGCAGAGTACGCGCTGGGGAGTGATCTTCATCAAGATGGAGGTTTGCTTCTGAATAACCCTTCG GCCTTGGCCCTTCACGAATGTAAATGCATCTGGCCAGATACGCCACTGGAGTGTATAGTATCCTTGGGCA CAGGACGGTATGAGAGTGATGTGAGGAATACCTCAACATACACGAGCCTGAAGACCAAGCTCTCTAATGT TATCAGCAGTGCTACAGATACAGAGGAAGTTCATATCATGCTTGATGGCCTGTTGCCTTCTGACACGTAC TTTAGATTTAATCCTGTGATATGTGAAAACATACCCCTAGATGAAAGCCGGGATGAAAAACTGGACCAGC TTCAGCTGGAAGGGATGAAGTATATAGAAAGAAATGATCAAAAGATGAAAAAAGTTGCAAAAATATTAAG TCAAGAAAAAACAACTCTTCAGAAGATCAATGATTGGATAAAATTAAAAAGTGACATGTATGAAGGCCTT CCATTTTTTTCAAAATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225706 representing NM_026164
Red=Cloning site Green=Tags(s) MSINLTLDIYIYFLNNARSLCGKQRSKQLHFVCSKQYWRMNHVNVHREFHTSKKSCKWNRSEAHCSKHWH SPSNHGLHFGIVRLSTSAPKGLTKVSIHMSRIKSTLNSVSKAIFGSQNEMVTRLAQFKPSSRILRKVSDK GWLKQKNVKQAVESLKNYSDKSAGKNSLAEQKSYFADKEEDSGKHSLFHYTYGITTRFGESFSVLANHIN SYFKSKGKMSQTKEDKQLQDKPDLEERKSSSPGPDTVADRPDSESPLEVKDKLSSPTQMPEAHPVSAKQS IANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSPPEEQEVSAKTEQAVNKDKKAEEKKRVLLQQEKIIAR VSIDNRTRALVQALRRTADPKLCITRVEELTFHLLEFPEGKGVAIKEKIIPYLLRLRQVKDETLQAAVRE ILALIGYVDPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFH MPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSNTWEKILKDRIGSALMIETARNPACPKVAAIS TIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGSDLHQDGGLLLNNPS ALALHECKCIWPDTPLECIVSLGTGRYESDVRNTSTYTSLKTKLSNVISSATDTEEVHIMLDGLLPSDTY FRFNPVICENIPLDESRDEKLDQLQLEGMKYIERNDQKMKKVAKILSQEKTTLQKINDWIKLKSDMYEGL PFFSKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026164 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026164.2, NP_080440.2 |
| RefSeq Size | 4425 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 67452 |
| UniProt ID | Q8K1N1 |
| MW | 87.8 kDa |
| Gene Summary | Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221497 | Pnpla8 (untagged) - Mouse patatin-like phospholipase domain containing 8 (Pnpla8), (10ug) |
CNY 9,980.00 |
|
| MG225706 | Pnpla8 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 8 (Pnpla8), (10ug) |
CNY 7,410.00 |
|
| MR225706L3 | Lenti ORF clone of Pnpla8 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 8 (Pnpla8) |
CNY 8,650.00 |
|
| MR225706L4 | Lenti ORF clone of Pnpla8 (mGFP-tagged) - Mouse patatin-like phospholipase domain containing 8 (Pnpla8) |
CNY 8,650.00 |
