Rest (NM_011263) Mouse Tagged ORF Clone
CAT#: MR225202
- TrueORF®
Rest (Myc-DDK-tagged) - Mouse RE1-silencing transcription factor (Rest)
ORF Plasmid: tGFP
"NM_011263" in other vectors (4)
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CNY 7,536.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610008J04Rik; AA407358; NRSF; REST4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225202 representing NM_011263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCAGGTGATGGGGCAGTCTTCTGGAGGAGGCAGTCTCTTCAACAACAGTGCCAACATGGGCA TGGCCTTAACCAACGACATGTACGACCTGCACGAGCTCTCGAAAGCTGAACTGGCAGCCCCTCAGCTCAT CATGTTAGCCAACGTGGCCCTGACGGGGGAGGCAAGCGGCAGCTGCTGCGATTACCTGGTCGGTGAAGAG AGGCAGATGGCCGAATTGATGCCCGTGGGAGACAACCACTTCTCAGAAAGTGAAGGAGAAGGCCTGGAAG AGTCGGCTGACCTCAAAGGGCTGGAAAACATGGAACTGGGAAGTTTGGAGCTAAGTGCTGTAGAACCCCA GCCCGTATTTGAAGCCTCAGCTGCCCCAGAAATATACAGCGCCAATAAAGATCCCGCTCCAGAAACACCC GTGGCGGAAGACAAATGCAGGAGTTCTAAGGCCAAGCCCTTCCGGTGTAAGCCTTGCCAGTACGAAGCCG AATCTGAAGAGCAGTTTGTGCATCACATCCGGATTCACAGCGCTAAGAAGTTCTTTGTGGAGGAAAGTGC AGAGAAACAGGCCAAAGCCTGGGAGTCGGGGTCGTCTCCGGCCGAAGAGGGCGAGTTCTCCAAAGGCCCC ATCCGCTGTGACCGCTGTGGCTACAATACCAACCGGTATGACCACTACATGGCACACCTGAAGCACCACC TGCGAGCTGGCGAGAACGAGCGCATCTACAAGTGCATCATCTGCACGTACACGACGGTCAGCGAGTACCA CTGGAGGAAACACCTGAGAAACCATTTCCCCAGGAAAGTCTACACCTGCAGCAAGTGCAACTACTTCTCA GACAGAAAAAATAACTACGTTCAGCACGTGCGAACTCACACAGGAGAACGCCCGTATAAATGTGAACTTT GTCCTTACTCAAGCTCTCAGAAGACTCATCTAACGCGACACATGCGGACTCATTCAGGTGAGAAGCCATT TAAATGTGATCAGTGCAATTATGTGGCCTCTAATCAGCATGAAGTGACCCGACATGCAAGACAGGTTCAC AACGGGCCTAAACCTCTTAATTGCCCGCACTGTGACTACAAAACAGCAGATAGAAGCAACTTCAAAAAGC ACGTGGAGCTGCATGTTAACCCACGGCAGTTCAACTGCCCCGTGTGTGACTACGCGGCTTCTAAGAAGTG TAATCTACAATACCATTTCAAATCTAAGCATCCCACCTGTCCCAGCAAAACAATGGATGTCTCCAAAGTG AAGCTAAAGAAAACCAAAAAGAGAGAGGCTGACCTGCTTAATAACGCCGTCAGCAACGAGAAGATGGAGA ATGAGCAAACAAAAACAAAGGGGGATGTGTCTGGGAAGAAGAACGAGAAACCTGTAAAAGCTGTGGGAAA AGATGCTTCAAAAGAGAAGAAGCCTGGTAGCAGTGTCTCAGTGGTCCAGGTAACTACCAGGACTCGGAAG TCAGCGGTGGCGGCGGAGACTAAAGCAGCAGAGGTGAAACACACAGACGGACAAACAGGAAACAATCCAG AAAAGCCCTGTAAAGCCAAGAAAAACAAAAGAAAGAAGGATGCTGAGGCCCATCCCTCCGAAGAGCCTGT GAACGAGGGACCAGTGACAAAAAAGAAAAAGAAGTCTGAGTGCAAATCAAAAATCGGTACCAACGTGCCA AAGGGCGGCGGCCGAGCGGAGGAGAGGCCGGGGGTCAAGAAGCAAAGCGCTTCCCTTAAGAAAGGCACAA AGAAGACGCCGCCCAAGACAAAGACAAGTAAAAAAGGTGGCAAACTTGCTCCAAAGGGGATGGGGCAGAC AGAACCTTCTTCTGGGGCATTGGCTCAAGTGGGGGTGTCTCCAGACCCTGCCCTCATTCAGGCAGAGGTC ACCGGGTCAGGATCTTCTCAGACAGAGCTTCCTTCACCCATGGATATTGCTAAGTCAGAGCCCGCCCAGA TGGAGGTTTCCCTAACAGGGCCACCTCCGGTGGAGCCTGCTCAAATGGAGCCATCGCCTGCGAAACCTCC CCAGGTAGAAGCACCCACTTACCCCCAGCCTCCCCAAAGGGGGCCTGCCCCTCCCACGGGGCCTGCCCCT CCCACGGGGCCTGCCCCTCCCACGGAGCCTGCCCCTCCCACGGGGCTTGCCGAGATGGAACCTTCTCCCA CGGAGCCTTCCCAGAAGGAACCACCTCCCAGTATGGAGCCTCCCTGCCCCGAGGAGCTGCCTCAGGCCGA GCCACCTCCTATGGAGGATTGTCAGAAGGAGCTGCCTTCTCCCGTGGAGCCCGCTCAGATTGAGGTTGCT CAGACGGCCCCTACGCAGGTTCAGGAGGAGCCCCCTCCTGTCTCGGAGCCACCTCGGGTGAAGCCAACCA AAAGATCATCTCTCCGGAAAGACAGAGCAGAGAAGGAGCTGAGCCTGCTGAGTGAGATGGCGCGGCAGGA GCAGGTCCTCATGGGGGTTGGCTTGGTGCCTGTTAGAGACAGCAAGCTTCTGAAGGGAAACAAGAGCGCC CAGGACCCCCCAGCCCCACCGTCACCATCGCCAAAGGGAAACTCGAGGGAAGAGACACCCAAGGACCAAG AAATGGTCTCTGATGGGGAAGGAACTATAGTATTCCCTCTCAAGAAAGGAGGACCAGAGGAAGCTGGAGA GAGTCCAGCTGAGTTGGCTGCTCTCAAGGAGTCTGCCCGTGTTTCATCCTCTGAACAAAACTCAGCCATG CCAGAGGGTGGAGCATCACACAGCAAGTGTCAGACTGGCTCCTCTGGGCTTTGTGACGTGGACACTGAGC AGAAGACAGATACTGTCCCCATGAAAGACTCCGCAGCAGAGCCAGTGTCCCCTCCTACCCCAACAGTGGA CCGTGACGCAGGGTCACCAGCTGTAGTGGCCTCCCCTCCTATCACGTTGGCTGAAAACGAGTCTCAGGAA ATTGATGAAGATGAAGGCATCCATAGCCATGATGGAAGTGACCTGAGTGACAACATGTCTGAGGGGAGTG ACGACTCAGGACTGCACGGGGCTCGGCCGACACCACCAGAAGCTACGTCAAAAAATGGGAAGGCAGGGTT GGCTGGTAAAGTGACTGAGGGAGAGTTTGTGTGTATTTTCTGTGATCGTTCTTTTAGAAAGGAAAAAGAT TATAGCAAACACCTCAATCGCCACTTGGTGAATGTGTACTTCCTAGAAGAAGCAGCTGAGGAGCAGGAGG AGCAGGAGGAGCGGGAGGAGCAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225202 representing NM_011263
Red=Cloning site Green=Tags(s) MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEE RQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETP VAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGP IRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFS DRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVH NGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKV KLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRK SAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVP KGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEV TGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAP PTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVA QTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSA QDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAM PEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQE IDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKD YSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011263 |
ORF Size | 3246 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011263.2, NP_035393.2 |
RefSeq Size | 4266 bp |
RefSeq ORF | 3249 bp |
Locus ID | 19712 |
UniProt ID | Q8VIG1 |
MW | 118.2 kDa |
Gene Summary | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells (PubMed:29961578, PubMed:9771705). Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes (By similarity). Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity). Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 2 (PubMed:21884984). Repressor activity may be inhibited by forming heterodimers with isoform 2, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes (PubMed:10490617, PubMed:11039732). Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (PubMed:15907476). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons (PubMed:27819263). Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (PubMed:25727884). Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions (By similarity). May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response (PubMed:24670762). Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223476 | Rest (untagged) - Mouse RE1-silencing transcription factor (Rest), (10ug) |
CNY 13,870.00 |
|
MG225202 | Rest (tGFP-tagged) - Mouse RE1-silencing transcription factor (Rest), (10ug) |
CNY 9,220.00 |
|
MR225202L3 | Lenti ORF clone of Rest (Myc-DDK-tagged) - Mouse RE1-silencing transcription factor (Rest) |
CNY 10,260.00 |
|
MR225202L4 | Lenti ORF clone of Rest (mGFP-tagged) - Mouse RE1-silencing transcription factor (Rest) |
CNY 10,260.00 |