Zfp322a (NM_172586) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Znf322; Znf322a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224717 representing NM_172586 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACCACTTAGGAGAGAACCATAGAAGATGTACACTTCAGAAGAGGAATGTAACCAGAGAACTCA AAAAAGGAAAACATACCATGTATGCCCTCAAAAGGGTGAAAAAGATTTATATTCGTGTACATGAGATTAC TCAAATAGATAATCAAACATATCAGTGCCTTGAACGTGAACAAAACTTTTGTGAAAACTTAGCACGAATG TGTGAGAGAACATATACCGAGGAGAAACCTTATAGATGTGATATGTGTGAGAAAACCTTCATCCAAAGTT CAGATCTTATTTCCCACCAGAGGATCCATAATTACGAGAAACCTTATAAGTGTAGCAAATGTGAGAAGAG CTTTTGGCACCACTTAGCCCTTTCAGGACACCAGAGAACACATGCAGGTAAAAAGTTTTATACCTGTGAC ATCTGTGGCAAGAATTTTGGTCAGAGCTCTGATCTGCTTGTCCACCAGCGAAGCCATACAGGTGAAAAAC CTTATCTGTGTAATGAGTGTGATAAATGCTTCAGTCGAAGTACAAATCTCATAAGGCACCGAAGAACTCA CACAGGTGAGAAACCGTTTAAGTGTCTGGAATGTGAAAAAGCTTTTAGTGGGAAATCAGATCTTATTAGC CACCAAAGGACTCATACCGGTGAAAGGCCCTACAAATGCAATAAATGTGAGAAAAGTTACCGACACCGGT CTGCCTTCATTGTGCATAAAAGAGTTCATACTGGGGAGAAGCCGTACAAGTGTGGTGCCTGTGAGAAGTG CTTTGGCCAGAAATCAGACCTTATTGTACACCAGAGAGTCCATACAGGAGAGAAACCATATAAATGCTTG GAGTGTATGAGAAGTTTTACTCGAAGTGCCAACCTAATTAGGCACCAGGCAACTCACACTCATACTTTCA AATGCCTTGAATATGAGAAAAGTTTCAACTGTAGCTCAGACTTTATTGTACATCAGAGAATTCATATGGA AGAAAAACCACATCAATGGTCTATGTGTGAGAGTGACTTCCTCCTAGGTATGGACTTCGTTGCCCAGCAG AAAATGAGGGCTCAAACAGAGGAGCTCCATTATAAATACAGTGTATGTGATAAAACCTTTCATCATAGCT CAGCCCTTCTTCAACATCAGACAGTGCACATTGATGATGAATACATCTGTAACATGAGTGAAAAAGGGCT TGATCTAAGTTCTCACGCATCGGAAACCTCACGTGTGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224717 representing NM_172586 Red=Cloning site Green=Tags(s) MENHLGENHRRCTLQKRNVTRELKKGKHTMYALKRVKKIYIRVHEITQIDNQTYQCLEREQNFCENLARM CERTYTEEKPYRCDMCEKTFIQSSDLISHQRIHNYEKPYKCSKCEKSFWHHLALSGHQRTHAGKKFYTCD ICGKNFGQSSDLLVHQRSHTGEKPYLCNECDKCFSRSTNLIRHRRTHTGEKPFKCLECEKAFSGKSDLIS HQRTHTGERPYKCNKCEKSYRHRSAFIVHKRVHTGEKPYKCGACEKCFGQKSDLIVHQRVHTGEKPYKCL ECMRSFTRSANLIRHQATHTHTFKCLEYEKSFNCSSDFIVHQRIHMEEKPHQWSMCESDFLLGMDFVAQQ KMRAQTEELHYKYSVCDKTFHHSSALLQHQTVHIDDEYICNMSEKGLDLSSHASETSRVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_172586
ORF Size	1230 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172586.4, NP_766174.1
RefSeq Size	4844 bp
RefSeq ORF	1233 bp
Locus ID	218100
UniProt ID	Q8BZ89
MW	48.5 kDa
Gene Summary	Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.[UniProtKB/Swiss-Prot Function]

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