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Slc8b1 (NM_133221) Mouse Tagged ORF Clone
CAT＃: MR223948
- TrueORF®
Slc24a6 (Myc-DDK-tagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 6 (Slc24a6), transcript variant 1
ORF Plasmid: tGFP
"NM_133221" in other vectors (4)
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CNY 4,360.00
CNY 5,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AF261233; NCKX6; NCLX; Slc24a6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223948 representing NM_133221
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCAGATGGCTGGCTCTGCTCTGGGCTCCTGTCTTCCTCTGTGTGGCTCTGATACTGGAGACGG CGTCTGGGACCGGAGACCCATCCACAAAAGCCCATGGACACATCCAGTTTTCAGCTGGAAGTGTCAACCA GACTGCCATGGCGGATTGCCGAGCCGTGTGTGGCCTGAACACATCTGATCGCTGTGACTTTGTCAGGAGG AATCCGGACTGCCGCAGCGAGGCGGGCTACCTGGACTACCTTGAGGGCATCTTCTGCTACTTCCCCCCCA ACCTCCTCCCTCTGGCCATCACCCTCTACGTTTTCTGGCTGCTTTACCTCTTTCTGATCCTGGGAGTCAC CGCGGCCAAGTTCTTCTGCCCTAACCTGTCAGCCATCTCCACTAACCTCAAACTCTCCCACAACGTGGCA GGTGTCACCTTCCTGGCCTTTGGAAATGGCGCTCCAGACATCTTCAGTGCTTTAGTGGCTTTCTCAGACC CACGTACTGCCGGCCTGGCCATCGGGGCTCTGTTTGGTGCAGGGGTGCTGGTCACCACTGTGGTGGCTGG AGGCATCACCATCCTGCATCCCTTCATGGCTGCCTCCAGGCCCTTCCTCAGGGACATCGCTTTCTACATG GTGGCTGTGTTCCTAACCTTCACTGCACTCTATCTCGGCAGGATCACGCTGACGTGGGCGCTGGGTTACC TGGGCCTCTACGTGTTCTACGTGGTCACGGTCATCATCTGCACTTGGGTCTACCAACGGCAGCGAAGCAG GTCTCTGGTCCACTCCATATCGGAGACACCAGAGTTGCTGTCTGAGTCAGAGGAGGACCAGATGTCTTCC AACACCAACAGCTATGACTATGGAGATGAATACCGGCCTCTGTTGCTGGGTCGGGAGACCACTGTCCAGA TCCTGATCCAAGCCCTGAATCCCTTGGACTACAGGAAGTGGAGAACTCAGTCGATATCCTGGAGGGTCCT GAAGGTAGTCAAGTTGCCTGTGGAGTTCTTGTTGCTGCTCACAGTACCGGTTGTGGACCCCGACAAGGAC GATCGGAATTGGAAACGGCCACTCAACTGTCTGCAGCTGGTCATCAGCCCCCTGGTCCTGGTCCTGACCC TGCAGTCGGGGGTCTATGGCATCTATGAGATCGGCGGTCTCCTTCCTGTCTGGGCTGTGGTGGTGATCGT GGGCACAGCGCTGGCTTCAGTGACCTTCTTTGCCACGTCTAACAGAGAACCCCCTAGACTGCACTGGCTC TTTGCTTTCCTGGGTTTCCTGACCAGTGCCCTGTGGATCAATGCAGCCGCCACAGAGGTGGTGAACATAT TACGGTCCCTGGGTGTGATCTTCCGCCTGAGCAACACCGTCCTAGGGCTGACCCTCCTGGCCTGGGGAAA CAGCATTGGAGATGCCTTCTCAGATTTCACGCTGGCCCGCCAAGGATACCCTCGGATGGCCTTCTCCGCC TGTTTCGGGGGCATCATCTTCAACATCCTGGTTGGTGTGGGGCTGGGCTGCTTGCTGCAGATCATCAGGA ACCACGTTGTGGAGGTGAAGCTGGAGCCAGACGGATTACTGGTGTGGGTGCTGGCCAGTGCCCTGGGCCT CAGCTTGATCTTCTCCCTGGTCTCCGTGCCGCTTCAGTGTTTCCAGCTCAGCAAGGCTTACGGCCTCTGC CTCCTCCTCTTCTACATCTGTTTCCTTGTTGTGGTCCTGCTCACAGAGTTTGGGGTGATTCACCTGAAGA AGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223948 representing NM_133221
Red=Cloning site Green=Tags(s) MASRWLALLWAPVFLCVALILETASGTGDPSTKAHGHIQFSAGSVNQTAMADCRAVCGLNTSDRCDFVRR NPDCRSEAGYLDYLEGIFCYFPPNLLPLAITLYVFWLLYLFLILGVTAAKFFCPNLSAISTNLKLSHNVA GVTFLAFGNGAPDIFSALVAFSDPRTAGLAIGALFGAGVLVTTVVAGGITILHPFMAASRPFLRDIAFYM VAVFLTFTALYLGRITLTWALGYLGLYVFYVVTVIICTWVYQRQRSRSLVHSISETPELLSESEEDQMSS NTNSYDYGDEYRPLLLGRETTVQILIQALNPLDYRKWRTQSISWRVLKVVKLPVEFLLLLTVPVVDPDKD DRNWKRPLNCLQLVISPLVLVLTLQSGVYGIYEIGGLLPVWAVVVIVGTALASVTFFATSNREPPRLHWL FAFLGFLTSALWINAAATEVVNILRSLGVIFRLSNTVLGLTLLAWGNSIGDAFSDFTLARQGYPRMAFSA CFGGIIFNILVGVGLGCLLQIIRNHVVEVKLEPDGLLVWVLASALGLSLIFSLVSVPLQCFQLSKAYGLC LLLFYICFLVVVLLTEFGVIHLKKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133221 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133221.2, NP_573484.2 |
| RefSeq Size | 2893 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 170756 |
| UniProt ID | Q925Q3 |
| MW | 64.8 kDa |
| Gene Summary | Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, by mediating the exchange of 3 sodium ions per 1 calcium ion (PubMed:20018762, PubMed:28445457). Plays a central role in mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion: calcium efflux is essential for mitochondrial function and cell survival, notably in cardiomyocytes (PubMed:24067497, PubMed:28445457). Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses (By similarity). Required for store-operated Ca(2+) entry (SOCE) and Ca(2+) release-activated Ca(2+) (CRAC) channel regulation: sodium transport by SLC8B1 leads to promote calcium-shuttling that modulates mitochondrial redox status, thereby regulating SOCE activity (By similarity). Involved in B-lymphocyte chemotaxis (PubMed:27328625). Able to transport Ca(2+) in exchange of either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange (By similarity). In contrast to other members of the family its function is independent of K(+) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Distinct Properties of Ca2+ Efflux from Brain, Heart and Liver Mitochondria: The Effects of Na+, Li+ and the Mitochondrial Na+/Ca2+ Exchange Inhibitor CGP37157
,Rysted, J;Lin, Z;Walters, G;Rauckhorst, A;Noterman, M;Liu, G;Taylor, E;Strack, S;Usachev, Y;,
Cell Calcium
,PubMed ID 33684833
[SLC8B1]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219330 | Slc24a6 (untagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 6 (Slc24a6), transcript variant 1, (10ug) |
CNY 7,410.00 |
|
| MG223948 | Slc24a6 (tGFP-tagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger) member 6 (Slc24a6) transcript variant 1, (10ug) |
CNY 5,610.00 |
|
| MR223948L3 | Lenti ORF clone of Slc24a6 (Myc-DDK-tagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 6 (Slc24a6), transcript variant 1 |
CNY 7,030.00 |
|
| MR223948L4 | Lenti ORF clone of Slc24a6 (mGFP-tagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 6 (Slc24a6), transcript variant 1 |
CNY 7,030.00 |
