Arid2 (NM_175251) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700124K17Rik; 4432409D24Rik; zipzap/p200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223666 representing NM_175251 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTCGACGGGGAAGGCGCCTCCGGACGAGCGGAGGAAGGGACTGGCTTTCCTGGACGAGCTGC GGCAGTTCCACCACAGCAGAGGGTCGCCGTTTAAGAAGATCCCTGCGGTGGGTGGGAAGGAGCTGGATCT TCACGGGCTCTACACCAGAGTCACTACTTTAGGCGGATTCGCGAAGGTTTCTGAGAAGAATCAGTGGGGA GAAATTGTTGAAGAGTTCAACTTTCCCAGAAGTTGTTCCAACGCTGCCTTTGCTTTAAAACAGTATTACT TGCGTTATCTAGAAAAGTACGAGAAAGTTCATCATTTTGGGGAAGATGATGATGAGGTACCACCAGGCAA TCCAAAGCCACAGCTTCCTATTGGTGCAATCCCATCTTCCTACAATTACCAGCAACACAGCGTGTCAGAT TATCTACGTCAAAGTTATGGGTTATCTATGGATTTTAATTCGCCAAATGATTATAATAAACTGGTGCTTT CACTGTTATCTGGACTCCCAAATGAAGTGGACTTCGCTATTAATGTGTGCACTCTCCTATCAAATGAAAG CAAGCACGTCATGCAGCTTGAGAAGGATCCCAAAATCATCACTTTACTGCTCGCTAATGCGGGGGTGTTC GATGACACTTTAGGATCATTCTCTTCTGTCTTTGGAGAAGAGTGGCGAGAGAAGACTGATAGAGACTTTG TTAAGTTTTGGAAAGACATTGTTGATGACAATGAAGTGCGAGATCTCATTTCTGACAGAAACAAGGCTCA TGAAGATACACCAGGAGAATGGATTTGGGAATCTTTATTTCATCCACCTCGAAAGCTGGGCATTAATGAC ATCGAAGGCCAGCGGGTTCTGCAGATCGCAGTGATCTTGCGGAACCTCTCCTTTGAGGAGAGCAATGTTA AGCTCTTGGCAGCTAATCGCACCTGTCTGCGTTTCCTGTTGCTCTCTGCACACAGTCATTTTATTTCATT AAGGCAGCTAGGCCTGGACACCTTAGGGAATATCGCAGCTGAGCTTTTACTGGACCCTGTGGATTTCAGA ACCACTCATCTGATGTTTCACACTGTTACAAAATGCCTGATGTCAAGGGATAGGTTTTTAAAGATGAGGG GCATGGAAATTTTGGGAAATCTCTGCAAAGCAGAGGATAACGGTGTTTTGATTTGTGAATATGTGGATCA AGATTCCTATAGAGAGATAATTTGTCACCTCACTCTGCCCGATGTGCTGCTGGTGACCTCAACCCTGGAG GTGCTGTACATGCTCACTGAAATGGGGGACGTGGCCTGCACAAAGATCGCGAAAGTGGAGAAGAGCATAG ACGTGCTGGTGTGTCTGGTCTCTATGGACGCTCAGATGTTTGGACCTGACGCACTTGCTGCCGTGAAGCT CATTGAGCATCCGAGCTCCAGTCACCAAGTGTTATCAGAGATTAGGCCGCAAGCCATAGAGCAGGTCCAA ACCCAGACCCACATAGCCTCCGGTCCAGCTTCCAGAGCAGTTGTAGCACAGCATGCTGCCCCCCCTCCAG GAATCGTGGAAATAGACAGTGAGAAGTTCGCTTGTCAGTGGCTAAATGCTCATTTTGAAGTAAATCCAGA CTGTTCCGTCTCTCGGGCAGAAATGTATTCAGAGTACCTCTCAACTTGCAGTAAATTAGCTCGCGGTGGC ATCCTCACATCAACTGGGTTTTATAAGTGTCTTAGAACAGTTTTTCCAAATCATACAGTGAAGAGGGTAG AAGATTCCACTAGCAGTGGGCAGGCGCATATCCATGTCATAGGAGTGAAGCGGCGGGCTCTCCCGCTCCC CATCCAGATGTACTATCAGCAGCAGCCAATTTCCACTCCTGTTGTCCGTGTTGATGCTGTTGCTGATCTA TCTCCAACTCCTTCACCTGCAGGAATCCCTCATGGACCACAGGCTGCAGGGAATCATTTTCAGAGGACTC CTGTCACCAATCAATCTTCAAATTTGACTGCAACACAAATGTCTTTTCCGGTACAAGGCATTCATACTGT GGCACAGACTGTTTCCAGAATTCCACCAAATCCTTCAGTTCATACCCACCAGCAACAAAATTCTCCAGTA ACTGTCATTCAGAATAAAGCTCCAATTCCTTGTGAAGTCGTTAAGGCAACAGTAATCCAGAACTCTGTGC CCCAGACGGCAGTTCCTGTGAGTATCTCTGTTGGAGGAGCACCTGCACAGAATTCTGTGGGTCAGAACCA TAGTGCAGGGCCACAGCCTGTTACAGTTGTAAATTCTCAGACATTACTTCACCATCCTTCTGTGATGCCA CAGCCATCTCCACTACACACAGTGGTGCCCGGACAGGTCCCTTCAGGCACTCCTGTCACAGTAATCCAGC AGACTGTACCGCAGAGTCGTATGTTTGGACGAGTACAGAGCATACCAGCGTGTACATCTACCGTCTCACA GGGTCAGCAGTTAATCACCACATCACCACAGCCTATGCACACTTCATCTCAACAGACAGCAGCTGGTAGC CAGCCACAAGACACTGTTATCATAGCACCCCCACAGTACGTAACAACTTCTGCATCCAATATCGTCTCAG CGACTTCAGTACAGAATTTCCAGGTAGCTACAGGACAGGTGGTTACCATAGCTGGTGTCCCGAGCCCACA GCCCTCCAGGGTAGGATTCCAGAACATTGCGCCCAAGCCACTTCCTTCTCAGCAAGTTTCACCATCAGTG GTCCAGCAGCCTATTCAACAACCACAGCAGCCTGCTCAGCAGAGTGTAGTGATTGTGAGCCAGCCAGCAC AGCAAGGCCAGGCGTACGCACCAGCCATTCACCAGATCGTTCTCGCTAACCCGGCAGCTCTCCCTGCCGG TCAGACGGTTCAGCTAACTGGACAACCAAACATAACTCCATCGTCATCACCATCACCTGTCCCGCCTACT AATAACCAAGTCCCTACTGCCATGTCATCTTCTTCCACCCTTCAGTCACAGGGACCCCCTCCTACTGTCA GTCAGATGCTCTCTGTGAAGAGGCAGCAGCAGCAGCAGCACTCACCAGCAGCGCCAGCACAGCAGGTCCA GGTCCAGGTTCAGCAGCCGCAGCAGGTCCAGGTGCAAGTTCAGCCGCAGCAACCGAGTGCTGGGGTCGGT CAGCCTGCTCCCAACGAGTCTAGTCTCATCAAGCAGCTGCTGCTGCCAAAGCGGGGCCCTTCAACCCCAG GGGGCAAGCTTATCCTCCCAGCCCCTCAGATTCCTCCCCCTAACAATGCAAGAGCTCCTAGCCCTCAGGT GGTCTATCAGGTGGCCAATAACCAAGCAGCTGGTTTTGGAGTGCAGGGGCAAACTCCGGCTCAGCAGCTA TTGGTTGGGCAGCAAAATGTTCAGTTGGTCCAAAGTGCAATGCCACCCGCAGGGGGAGTGCAAACCGTGC CCATTTCGAACTTACAAATATTGCCGGGTCCGCTGATCTCAAACAGCCCAGCAACCATTTTCCAAGGGAC TTCTGGCAACCAGGTAACTATAACAGTTGTGCCAAATACCAGTTTTGCAACTGCGACTGTGAGTCAGGGA AACGCTGCTCAGCTCATTGCGCCAGCCGGTCTTAGCATGAGCGGAGCGCAGGCAAGCGCTGGACTTCAGG TGCAGACGCTTCCAGCCGGACAATCAGCGTGTACCACTGCTCCCCTCCCGTTCAAAGGCGACAAGATCAT TTGCCAAAAGGAGGAGGAGGCAAAGGAAGCAACAGGTCTACATGTTCATGAACGGAAGATTGAGGTCATG GAGAATCCTTCCTGTCGGCGAGGAACCACAAACACCAGCAACGGGGATACAAGTGAGAGTGAACTCCAGG TGGGAAGTCTTTTAAATGGGAGAAAGTATAGTGACTCAAGTCTACCTCCTTCAAACTCAGGGAAACTTCA GAGTGAGACGAGCCAGTGCTCACTAATCAGCAATGGGCCATCGTTGGAACTAGGTGAGAATGGAGCGCCT GGAAAACAGAACTCAGAACCAGTAGACATGCAGGATGTCAAAGGTGATCTGAAAAAAGCCCTCGTCAATG GAATCTGTGATTTTGATAAAGGAGATGGTTCTCATTTAAGCAAAAACATTCCAAATCACAAAACTTCTAA TCATGTAGGAAATGGTGAGATATCTCCAGTAGAACCACAAGGGACTTCGGGTGCCACTCAGCAAGATACT GCCAAAGGTGACCAACTAGAAAGAGTTTCTAATGGACCTGTGTTAACTCTGGGTGGGTCACCGTCCACAA GCAGTATGCAAGAAGCCCCGAGTGTGGCGACACCGCCGTTGAGTGGTACTGACCTGCCTAACGGACCTCT AGCTTCAAGTTTGAATTCAGATGTGCCTCAGCAACGCCCAAGTGTAGTTGTCTCACCACATTCTACAGCC CCTGTCATACAGGGGCATCAAGTCATAGCAGTTCCCCACTCAGGACCTAGAGTGACCCCTTCTGCTCTAT CATCTGATGCTCGGTCTACAAACGGCACAGCCGAGTGCAAAACTGTAAAGAGGCCGGCAGAGGATAATGA TAGGGACACTGTCCCGGGAATCCCAAATAAAGTAGGGGTTAGAATTGTTACAATCAGCGACCCCAACAAT GCTGGCTGCAGTGCAACCATGGTTGCGGTCCCAGCTGGAGCGGACCCAAGCACTGTAGCGAAAGTAGCAA TAGAAAGTGCTGCTCAGCAAAAGCAGCAGCATCCACCGACCTACATGCAGAGTGTGGCCCCACAGAACAC TCCTATGCCACCTTCACCAGCTGTACAAGTGCAGGGCCAGCCTAGCAGTTCTCAGCCTTCTCCAGTCAGT GCGTCCAGTCAGCATGCAGATCCAGTGAGAAAACCTGGGCAGAACTTCATGTGTCTGTGGCAGTCTTGTA AAAAGTGGTTTCAGACTCCCTCACAAGTGTTCTATCATGCAGCTACTGAACATGGAGGAAAAGATGTGTA TCCGGGGCAGTGTCTTTGGGAAGGCTGTGAGCCTTTCCAACGGCAGAGGTTCTCTTTCATTACCCACTTA CAGGATAAGCACTGTTCAAAGGATGCCCTGCTTGCAGGATTAAAGCAAGATGAACCAGGACAAGTGGCAA ATCAAAAATCTTCTACCAAGCAGCCCACCGTGGGGGGCACAGGCTCTGCGCCCAGAGCCCAGAAGGCCAT TGCAAGCCACCCCAGTGCTGCACTCATGGCTCTGCGGAGAGGCTCAAGGAACCTCGTCTTCCGGGACTTC ACAGATGAAAAAGAGGGACCAATAACTAAACACATCCGACTAACAGCTGCCTTAATATTAAAAAATATTG GTAAATACTCAGAGTGTGGGCGCAGATTGTTAAAGAGACATGAAAACAACTTATCAGTGCTCGCCATTAG TAACATGGAAGCTTCCTCTACCCTTGCCAAATGCCTTTATGAACTTAATTTTACAGTTCAGAGTAAAGAA CAAGAAAAAGACTCAGAAATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223666 representing NM_175251 Red=Cloning site Green=Tags(s) MANSTGKAPPDERRKGLAFLDELRQFHHSRGSPFKKIPAVGGKELDLHGLYTRVTTLGGFAKVSEKNQWG EIVEEFNFPRSCSNAAFALKQYYLRYLEKYEKVHHFGEDDDEVPPGNPKPQLPIGAIPSSYNYQQHSVSD YLRQSYGLSMDFNSPNDYNKLVLSLLSGLPNEVDFAINVCTLLSNESKHVMQLEKDPKIITLLLANAGVF DDTLGSFSSVFGEEWREKTDRDFVKFWKDIVDDNEVRDLISDRNKAHEDTPGEWIWESLFHPPRKLGIND IEGQRVLQIAVILRNLSFEESNVKLLAANRTCLRFLLLSAHSHFISLRQLGLDTLGNIAAELLLDPVDFR TTHLMFHTVTKCLMSRDRFLKMRGMEILGNLCKAEDNGVLICEYVDQDSYREIICHLTLPDVLLVTSTLE VLYMLTEMGDVACTKIAKVEKSIDVLVCLVSMDAQMFGPDALAAVKLIEHPSSSHQVLSEIRPQAIEQVQ TQTHIASGPASRAVVAQHAAPPPGIVEIDSEKFACQWLNAHFEVNPDCSVSRAEMYSEYLSTCSKLARGG ILTSTGFYKCLRTVFPNHTVKRVEDSTSSGQAHIHVIGVKRRALPLPIQMYYQQQPISTPVVRVDAVADL SPTPSPAGIPHGPQAAGNHFQRTPVTNQSSNLTATQMSFPVQGIHTVAQTVSRIPPNPSVHTHQQQNSPV TVIQNKAPIPCEVVKATVIQNSVPQTAVPVSISVGGAPAQNSVGQNHSAGPQPVTVVNSQTLLHHPSVMP QPSPLHTVVPGQVPSGTPVTVIQQTVPQSRMFGRVQSIPACTSTVSQGQQLITTSPQPMHTSSQQTAAGS QPQDTVIIAPPQYVTTSASNIVSATSVQNFQVATGQVVTIAGVPSPQPSRVGFQNIAPKPLPSQQVSPSV VQQPIQQPQQPAQQSVVIVSQPAQQGQAYAPAIHQIVLANPAALPAGQTVQLTGQPNITPSSSPSPVPPT NNQVPTAMSSSSTLQSQGPPPTVSQMLSVKRQQQQQHSPAAPAQQVQVQVQQPQQVQVQVQPQQPSAGVG QPAPNESSLIKQLLLPKRGPSTPGGKLILPAPQIPPPNNARAPSPQVVYQVANNQAAGFGVQGQTPAQQL LVGQQNVQLVQSAMPPAGGVQTVPISNLQILPGPLISNSPATIFQGTSGNQVTITVVPNTSFATATVSQG NAAQLIAPAGLSMSGAQASAGLQVQTLPAGQSACTTAPLPFKGDKIICQKEEEAKEATGLHVHERKIEVM ENPSCRRGTTNTSNGDTSESELQVGSLLNGRKYSDSSLPPSNSGKLQSETSQCSLISNGPSLELGENGAP GKQNSEPVDMQDVKGDLKKALVNGICDFDKGDGSHLSKNIPNHKTSNHVGNGEISPVEPQGTSGATQQDT AKGDQLERVSNGPVLTLGGSPSTSSMQEAPSVATPPLSGTDLPNGPLASSLNSDVPQQRPSVVVSPHSTA PVIQGHQVIAVPHSGPRVTPSALSSDARSTNGTAECKTVKRPAEDNDRDTVPGIPNKVGVRIVTISDPNN AGCSATMVAVPAGADPSTVAKVAIESAAQQKQQHPPTYMQSVAPQNTPMPPSPAVQVQGQPSSSQPSPVS ASSQHADPVRKPGQNFMCLWQSCKKWFQTPSQVFYHAATEHGGKDVYPGQCLWEGCEPFQRQRFSFITHL QDKHCSKDALLAGLKQDEPGQVANQKSSTKQPTVGGTGSAPRAQKAIASHPSAALMALRRGSRNLVFRDF TDEKEGPITKHIRLTAALILKNIGKYSECGRRLLKRHENNLSVLAISNMEASSTLAKCLYELNFTVQSKE QEKDSEML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175251
ORF Size	5484 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175251.4, NP_780460.3
RefSeq Size	9031 bp
RefSeq ORF	5487 bp
Locus ID	77044
UniProt ID	E9Q7E2
MW	196.4 kDa
Gene Summary	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.[UniProtKB/Swiss-Prot Function]

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