Cacna1b (NM_007579) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	alpha(1B); AW050276; AW060892; AW822256; BIII; Cav2.2; Cchn1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222880 representing NM_007579 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGTG GCGGGGCCGGCGGGGCGGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAGCGCGCACGGACTATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATACATGATCCTGGCCACCATCATCGCCAACTGCATTGTTCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAGCGACTAGATGACACGGAGCCTTACTTCATCGGGATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCCCTGGGCTTTGTTTTCCACAAGGGCTCCTACCTTCGGA ACGGCTGGAATGTCATGGACTTCGTGGTGGTCCTCACGGGGATTCTCGCCACAGCTGGAACTGACTTTGA CCTGCGCACACTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGCTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTTAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATTGGGCTGCTGCTCTTCTTTGCCA TCCTCATGTTTGCCATCATCGGCCTCGAATTCTATATGGGCAAATTCCATAAGGCCTGTTTCCCCAACAG CACAGATACAGAGCCTGTGGGTGACTTTCCCTGTGGCAAAGATCCCCCTGCTCGTCAGTGTGATGGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACAGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC TGGCAACACATGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTGGGTGTGCTTTCCGGAGAGTTTGCCAAGGAGCGGGAGCGAGTCGAGAACCGCCGCGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAGCTGAATGGGTACTTGGAGTGGATCTTCAAGGCAGAGGA AGTCATGTTGGCAGAGGAGGACAAGAATGCAGAAGAGAAATCCCCTTTGGATGTGTTGAAGAGAGCTGCC ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCAG TTGGGTCTCCATTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACGGAGAGCTCATCGTACTTCCGGAGAAA GGAGAAGATGTTCCGGTTCTTTATCCGGCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGT GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCACTATAATCAGCCTCAGCGGCTTACCACTGCAC TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTAGG GCCCAGAAGTTACTTCAGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTTGGAATCAGTGTGCTGCGGGCTCTGCGACTGCTGA GGATATTCAAAGTTACCAAGTATTGGAACTCTCTGAGGAACCTGGTGGTTTCCCTCCTCAATTCCATGAA GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTCTTCATCGTGGTCTTCGCTCTGTTGGGGATGCAGCTG TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC TCACTGTCTTTCAGATCCTGACAGGAGAGGATTGGAATGCCGTAATGTATCATGGGATTGAGTCGCAAGG TGGAGTCAGCAAAGGCATGTTTTCTTCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG CTGAATGTTTTTCTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG AGATGGAAGAAGCAGCCAATCAGAAACTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT GTCTGCTGCCAATATCTCCATCGCTGCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGG GCCAGTCAGCTAAGGCTCCAGAATCTGCGTGCCAGCTGTGAGGCATTGTACAGTGAGATGGACCCTGAGG AGCGCCTGCGTTATGCCAGCACGCGCCATGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGT GGTGGAGCCTGGTCGAGATGGCTTGCGGGGACCCGTTGGGAGCAAGTCAAAGCCTGAAGGCACGGAGGCC ACAGAAAGCGCGGACCTACCTCGCAGGCACCACCGGCACCGTGATAGGGACAAGACCTCAGCCACAGCAC CTGCTGGAGGCGAACAGGACAGGACAGAAAGCACCGAGACCGGGGCCCGGGAGGAACGTGCGCGCCCTCG TCGAAGTCACAGCAAGGAGACTCCAGGGGCTGACACGCAAGTGCGCTGTGAGCGCAGTAGACGTCACCAC CGGCGCGGCTCCCCGGAGGAGGCCACTGAACGGGAGCCTCGGCGCCACCGTGCCCACCGGCATGCACAGG ACTCAAGCAAGGAGGGCACGGCGCCGGTGCTTGTACCCAAGGGTGAGCGACGAGCAAGACACCGAGGCCC ACGCACGGGTCCACGTGAGGCAGAGAACAACGAGGAGCCCACACGCAGGCACCGTGCAAGGCATAAGGTG CCACCCACACTGCAGCCCCCAGAGAGGGAGGCTGCAGAGAAGGAGAGCAACGCGGTGGAAGGGGATAAGG AAACCCGAAATCACCAGCCCAAGGAACCTCACTGTGACCTGGAGGCCATTGCAGTTACAGGTGTGGGCCC TCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAGCAACCAGAGGATGCAGACAACCAGCGT AATGTCACCCGGATGGGCAGTCAGCCCTCAGATCCCAGCACCACTGTGCATGTCCCAGTGACACTGACAG GCCCTCCTGGGGAGACCCCTGTAGTTCCCAGTGGTAACATGAACCTGGAAGGCCAAGCAGAGGGCAAGAA GGAGGCAGAGGCGGATGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTCCCTACAGCTCCATGTTTTGT CTCAGCCCCACCAACCTGCTTCGTCGCTTCTGCCATTACATTGTGACCATGCGGTACTTTGAGATGGTAA TTCTTGTGGTCATTGCCTTGAGCAGCATTGCCCTGGCTGCAGAGGATCCTGTGCGGACAGATTCATTCAG GAACAACGCTTTAAAGTACATGGATTACATCTTTACAGGAGTCTTCACCTTTGAAATGGTCATAAAGATG ATAGACTTGGGCTTGCTGCTGCACCCTGGTGCCTACTTCCGGGACCTGTGGAACATTCTGGACTTCATTG TTGTCAGTGGAGCCCTGGTGGCATTTGCGTTCTCAGGATCCAAAGGGAAAGACATCAATACCATCAAGTC TCTGAGAGTCCTGCGTGTCCTGAGGCCCCTCAAGACCATCAAGCGGCTGCCTAAGCTCAAGGCTGTCTTT GACTGTGTGGTGAACTCCCTGAAGAACGTCTTGAACATCCTGATTGTCTACATGCTCTTCATGTTCATAT TTGCCGTCATTGCCGTCCAGCTCTTCAAAGGGAAGTTCTTTTACTGTACTGATGAATCCAAGGAGCTGGA GAGGGACTGCCGGGGTCAGTATTTGGATTATGAGAAGGAAGAAGTAGAAGCCCAGCCAAGGCAGTGGAAG AAATATGACTTCCACTATGACAATGTTCTCTGGGCCTTGTTGACGCTGTTCACAGTGTCCACGGGAGAGG GGTGGCCCATGGTGCTGAAACACTCTGTGGATGCCACCTATGAGGAACAGGGGCCCAGTCCCGGGTTCCG GATGGAGCTCTCCATCTTCTACGTGGTCTACTTTGTGGTCTTCCCTTTTTTCTTTGTCAACATCTTTGTG GCCTTGATCATTATCACCTTCCAGGAACAGGGAGATAAGGTGATGTCTGAATGCAGCTTAGAAAAGAATG AGAGGGCTTGCATTGATTTTGCCATCAGTGCCAAGCCCCTGACACGGTACATGCCTCAAAACAAACAGTC GTTCCAGTATAAGACATGGACATTCGTGGTCTCTCCACCCTTTGAGTACTTCATCATGGCTATGATAGCC CTCAACACAGTGGTGCTGATGATGAAGTTCTATGATGCACCTTATGAGTACGAGCTGATGCTGAAATGCC TGAACATTGTCTTCACATCCATGTTCTCGATGGAGTGCATACTGAAGATCATCGCCTTTGGGGTATTGAA CTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACGGTTTTGGGAAGTATTACTGATATTTTAGTA ACAGAGATAGCGGAAACGAACAACTTCATCAACCTAAGCTTCCTTCGCCTCTTCCGGGCGGCACGGCTGA TCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCCTATTGTGGACCTTCGTCCAGTCCTTTAAGGCGCT GCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCATCGGCATGCAGGTTTTTGGA AACATTGCCCTTGATGATGACACCAGTATCAACCGACACAACAACTTCCGGACATTTCTGCAAGCCTTAA TGCTATTGTTCAGGAGTGCCACTGGGGAGGCCTGGCATGAGATCATGCTGTCTTGTCTGGGCAACCGGGC CTGTGACCCACATGCCAACGCCAGTGAGTGCGGGAGCGACTTTGCCTATTTTTATTTTGTCTCCTTCATC TTCCTCTGTTCCTTTCTGATGTTGAACCTCTTTGTTGCTGTAATCATGGACAATTTTGAGTACCTCACTC GGGACTCTTCCATCCTAGGGCCTCACCACTTAGACGAATTCATTCGAGTCTGGGCTGAATACGACCCAGC TGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACATGTCCCCACCTCTGGGGTTG GGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGCATGAACATGCCCATATCCAATGAGG ACATGACGGTGCACTTTACGTCCACACTGATGGCCCTCATCCGGACAGCACTGGAGATCAAGCTTGCCCC AGCTGACGAGATGACAGTGGGGAAGGTCTATGCTGCTCTCATGATATTTGACTTCTACAAACAGAACAAA ACCACCAGAGATCAGACTCACCAAGCTCCCGGAGGCCTGTCCCAGATGGGTCCCGTTTCCCTGTTCCACC CTCTGAAGGCCACCCTGGAACAGACACAGCCCGCTGTGCTTCGAGGAGCTCGGGTTTTCCTTCGGCAAAA GAGTGCAACTTCCCTCAGCAATGGGGGTGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCGCTGTCC TGGGGCACGCAGAGGACCCAAGATGCACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAG AGATCCCTGTGGGGCAGTCAGGAACACTGGCTGTGGATGTCCAGATGCAGAACATGACACTGAGAGGACC AGATGGGGAGCCCCAGCCTGGGCTGGAAAGCCAAGGCAGAGCTGCCTCTATGCCACGCCTAGCGGCAGAA ACACAGCCGGCCCCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCACATGGGA CTCAGCTTTGCAGCACAGTTCTGGACCGGCCTCCTCCTAGCCAGGCATCACATCACCACCACCACCGCTG CCACCGGCGCAGAGACAAGAAGCAAAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACCCAGAAGGT GCACCAAGCACTGCTGCAGGACCTGGTCTGCCCCATGGAGAAGGATCCACCGCCTGCCGGCGGGACCGTA AACAGGAGCGAGGCCGGTCCCAGGAGCGGAGGCAGCCCTCATCTTCCTCTTCAGAGAAGCAGCGCTTCTA TTCCTGTGACCGCTTTGGGAGCCGGGAGCCCCCGCAACTGATGCCCTCACTCAGTAGCCACCCCACATCG CCAACAGCGGCGCTAGAGCCAGCACCCCACCCACAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGT CAACATCCGGTGCTAGCACCCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCTCTGACCCCACG CCCCAGCATCACCTACAAGACCGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGCGGCCTCCCA GCCTTCTCCCCTGGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGA GCCAGCCTCTAGCTCCTGGCTCCCGAATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGC CTCCGCCCACACCCTGCCTGAGGATACACTCACCTTTGAAGAGGCAGTGGCCACCAACTCTGGCCGCTCC TCCAGGACTTCCTATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTATC ACTGCACTTTGGGACTCAGCACTGGCGTCCGGGCGCGGCACAGCTACCACCACCCCGATCAGGACCACTG GTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222880 representing NM_007579 Red=Cloning site Green=Tags(s) MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFT VNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGI FCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQ VVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGD TECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNL VLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAA TKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLC VVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFE VVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQL FGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTL LNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQR ASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEA TESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHH RRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKV PPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQR NVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFC LSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKM IDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVF DCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWK KYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFV ALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIA LNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILV TEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG NIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFI FLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGL GKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPADEMTVGKVYAALMIFDFYKQNK TTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLS WGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAE TQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEG APSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTS PTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLP AFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRS SRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_007579
ORF Size	6864 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007579.3, NP_031605.2
RefSeq Size	7167 bp
RefSeq ORF	6867 bp
Locus ID	12287
MW	257.5 kDa
Gene Summary	Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by omega-conotoxin-GVIA (AC P01522) (By similarity). They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.[UniProtKB/Swiss-Prot Function]

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