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Lemd3 (NM_001081193) Mouse Tagged ORF Clone
CAT＃: MR221903
- TrueORF®
Lemd3 (Myc-DDK-tagged) - Mouse LEM domain containing 3 (Lemd3)
ORF Plasmid: tGFP
"NM_001081193" in other vectors (4)
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CNY 6,720.00
CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI316861; Man1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221903 representing NM_001081193
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGACGGCGGCGGCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCC GTTACGGCTTATCTCCGGGTCCCGTGACGGAGAGCACCCGGCCCGTCTACCTCAAGAAGCTGAAGAAGCT TCGCGAGGAAGAGCAGCAGCAACAGCAGCAACAGCAGCAGCAGCAGCACCGGGCGGGGGGCCGCGGCAAC AAGACGCGGAACAGTAATAACAATAACACGGCGACGGCGATGGGGGGCCGGCCGGGCTCCGGGGACCTCG CGTACTTACGGAGCCCCGCGGGCCTCGGCCGCCTGTCGGCCTCGGCTGCCGAGAGCCCCGTGGCAGGAGG CTCCGGGGGCGCGGCGGCCGTCCCCGCGGCGGGCAGCAAAGTGCTGCTGGGCTTCAGCTCGGACGAGTCG GACGTGGAGGCCAGCCCGCGGGAGCAGGCGGGCGGCGGCGGCGCGCGGAGGGACCGGGCTGCGCTCCAGT ACCGCGGGCTCAGAGCCCCACCGGCGCCCCCGGCCGCGGGCGAGGTGACGGGCGGCCACCCGGGCGAGCG GAGGAAGCCGCACTCGTGGTGGGGGGCGCGGAGGCCGGCGGGCCCCGAGCCGCAGCCCCCGGCGGCGGGG AGCGACGGGGCCGCGGAGGACGCGGACGAGGAGCTGGCGGACGGTGAGGACCGCGACCCCGAGGCCGAGG AGCCGCTGTGGGCCAGCCGAACGGTGAACGGCAGCCGGCTTCTCCCCTACAGCAGCTGCCGGGAGCACTA CTCGGACTCGGAGGAGGAGGAGGAGGAGGGGGAGGAGGACGGCGACGTGGCCCCGGCCAGACAGGTATTA AAGGACGACTCCCTCGCCCGGCATCGACCCAGACGGAGCCATAGTAAGCCGTTCTCGGCGCTGACTGCTA AATCTGGCGGCAGCCGGCAGGAGACTTCGGTTCAGGGAGGGGGAGCACTCGCGATGAATGACAGGGCGGC GGCCGCGGGGAGTCTAGACAGGAGCCGAAACCTCGAGGAGGCGGCGGCCGAGCCGGGAGGAGGAGGAGGA GGAGGGTGCGGGTGCGATCCAGTGGACTCCATTCCTCGATACCGTGCCGGCGCGAAGAAGCTGGCCCCGC TGCTGTCGCCTCCGAGTCCCGATGGGGACTCCACCTTGGAGTCGCCCACGGGGCCCCTCCTCAAAACCAA TAATCACATAGGCGGCGGGGCCTTCGGCGTGGATTCCCCCGGTCTGTATGCCAACAGCCTCCCTCCCGGG GCCACCGCCGCGGCCGCCCCTGGCACACTCAGGATCAACCACGCCAACCATACTGGCTCCAATCACACCT ACCTGAAAACCGCCTACGGCAAGCCGAAGCTCTGCGAGCCCGAAGAGGAACTTCTCCAGCAGTTTAAACG GGAGGAGGTGTCCCCGACGGGGAGTTTCAGCGCTCACTACTTGTCGATGTTCCTCCTAACCGCTGCCTGC TTGTTTTTCCTAATACTGGGACTGACTTACCTAGGAATGAGAGGGACGGGAGTACCCGAGGACGGAGGAC TCATAAAAAACCCCTTTGATGAAACATTTGGAAAAATACAAGAAAGTGAAAAAAATCTTTTGATGAGCAC ATTGTACAAACTTCATGATCGATTGGCCCAGATTGCAGGAGACCATGAATGTGGCAGTTCTAGTCAAAGG ATGCTTTCTGTCCAAGAAGCAGCTGCATATTTAAAAAATTTAGGTCCTGAGTATGAAGATGTATTTAATA CTTCATTGCTGTGGATTTTTAAAAATGGGAAAGATGTTGGAATAAGGTGTGTTGGTTACGGGCCTGAGGA AGACTTGACAAACATAACTGATGTGCAGTTTTTACAGTCCACCAGGCCCCAGATGCCCTTCTGGTGCCGT TTCCGGCGTGCTTTCATTACTGTGACCCATAGGTTATTGTTGTTATGTTTAGGTGTAGTGCTGGTGTGCG TTGCTCTCCGGTACATGAGATACCGCTGGACTAAGGAGGAGGAGGAGACGAGGCAGATGTATGACATGGT GGTAAAGATTATAGATGTTTTGCGAAGTCATAATGAAGCTTGCCAGGAGACTAAAGACTTACAGCCTTAC ATGCCTCTTCCACATGTCCGCGATTCCCTAATACAGCCGCAGGACAGGAAAAAGATGAAGAAAGTCTGGG ATAGAGCTGTTGACTTCCTTGCTGCTAACGAATCTAGAGTCCGTACGGAGACTCGAAGAGTAGGCGGTGC AGACTTTCTAGTCTGGCGGTGGATCCAGCCCTCTGCGTCTTGTGACAAGACATTAGTTATACCCTCTAAA GTATGGCAAGGTCAAGCATTTCATTTAGATAGAAGAAATTCACCACCAAATAGTTTGACTCCATGTTTAA AGATTCGAAATATGTTTGACCCGGTTATGGAAATAGGAGATCACTGGCACCTGGCGATCCAAGAAGCGAT TTTAGAGAAGTGCAGTGACAATGACGGCATCGTTCATATTGCCGTAGACAGGAACTCGCGGGAGGGCTGT GTCTATGTTAAATGTTTGTCTCCGGAATATGCTGGGAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTG ATGGAAAATTGGTTACAGTAAAATACTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCCCTCAC TTGCAACACTCCCTTGAAGCCAGCAAATAAACATATGAACTCTCTGTCTCACCTGCGCCTGCGGACTGGC CTAGCTAATTCTCAAGGAAGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221903 representing NM_001081193
Red=Cloning site Green=Tags(s) MAAATAAAAPQQLSDEELFSQLRRYGLSPGPVTESTRPVYLKKLKKLREEEQQQQQQQQQQQHRAGGRGN KTRNSNNNNTATAMGGRPGSGDLAYLRSPAGLGRLSASAAESPVAGGSGGAAAVPAAGSKVLLGFSSDES DVEASPREQAGGGGARRDRAALQYRGLRAPPAPPAAGEVTGGHPGERRKPHSWWGARRPAGPEPQPPAAG SDGAAEDADEELADGEDRDPEAEEPLWASRTVNGSRLLPYSSCREHYSDSEEEEEEGEEDGDVAPARQVL KDDSLARHRPRRSHSKPFSALTAKSGGSRQETSVQGGGALAMNDRAAAAGSLDRSRNLEEAAAEPGGGGG GGCGCDPVDSIPRYRAGAKKLAPLLSPPSPDGDSTLESPTGPLLKTNNHIGGGAFGVDSPGLYANSLPPG ATAAAAPGTLRINHANHTGSNHTYLKTAYGKPKLCEPEEELLQQFKREEVSPTGSFSAHYLSMFLLTAAC LFFLILGLTYLGMRGTGVPEDGGLIKNPFDETFGKIQESEKNLLMSTLYKLHDRLAQIAGDHECGSSSQR MLSVQEAAAYLKNLGPEYEDVFNTSLLWIFKNGKDVGIRCVGYGPEEDLTNITDVQFLQSTRPQMPFWCR FRRAFITVTHRLLLLCLGVVLVCVALRYMRYRWTKEEEETRQMYDMVVKIIDVLRSHNEACQETKDLQPY MPLPHVRDSLIQPQDRKKMKKVWDRAVDFLAANESRVRTETRRVGGADFLVWRWIQPSASCDKTLVIPSK VWQGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDHWHLAIQEAILEKCSDNDGIVHIAVDRNSREGC VYVKCLSPEYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTCNTPLKPANKHMNSLSHLRLRTG LANSQGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081193 |
| ORF Size | 2754 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081193.2, NP_001074662.2 |
| RefSeq Size | 4754 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 380664 |
| UniProt ID | Q9WU40 |
| MW | 100.6 kDa |
| Gene Summary | Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222616 | Lemd3 (untagged) - Mouse LEM domain containing 3 (Lemd3), (10ug) |
CNY 11,780.00 |
|
| MG221903 | Lemd3 (tGFP-tagged) - Mouse LEM domain containing 3 (Lemd3), (10ug) |
CNY 8,930.00 |
|
| MR221903L3 | Lenti ORF clone of Lemd3 (Myc-DDK-tagged) - Mouse LEM domain containing 3 (Lemd3) |
CNY 9,980.00 |
|
| MR221903L4 | Lenti ORF clone of Lemd3 (mGFP-tagged) - Mouse LEM domain containing 3 (Lemd3) |
CNY 9,980.00 |
