Dram1 (NM_027878) Mouse Tagged ORF Clone
CAT#: MR220640
- TrueORF®
Dram1 (Myc-DDK-tagged) - Mouse DNA-damage regulated autophagy modulator 1 (Dram1)
ORF Plasmid: tGFP
"NM_027878" in other vectors (4)
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CNY 1,416.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1200002N14Rik; Dram |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220640 representing NM_027878
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGCTTCCTCAGGGGAATGGCTTTCGTCCCCTTCCTCCTGGTGACTTGGTCGTCCGCAGCCTTCA TCATCTCCTACGTGGTCGCGGTGCTCTCTGGGCACGTCAACCCCTTTCTCCCCTATATCAGTGACACAGG AACAACTCCTCCAGAGAGTGGTATTTTTGGATTCATGATAAACTTCTCTGCATTTCTTGGCGCAGCTACG ATGTACACAAGATACAAGATAGTGGAGAAGCAGAATGAGACCTGCTACTTCAGCACTCCCGTTTTTAACT TGGTGTCCTTGGCGCTTGGATTGGTGGGATGCATCGGAATGGGCATCGTAGCCAACTTCCAGGAGTTAGC CGTGCCTGTGGTCCATGATGGCGGTGCGCTTCTGGCTTTCGTCTGCGGGGTGGTGTACACGCTCCTGCAA TCGATCATCTCCTACAAATCCTGTCCCCAGTGGAACAGTCTCACCACGTGCCATGTCAGGATGGCCATCT CCGCTGTTTCGTGCGCAGCTGTCGTCCCCATGATTGCCTGTGCTTCACTCATTTCTATAACCAAGCTGGA ATGGAATCCAAAAGAAAAGGATTATATATATCACGTGGTGAGCGCCATCTGTGAGTGGACCGTGGCTTTT GGTTTTATTTTCTATTTCCTAACATTCATCCAAGATTTCCAGAGTGTCACTCTAAGGATATCCACAGAAA TCAATGACGACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220640 representing NM_027878
Red=Cloning site Green=Tags(s) MLCFLRGMAFVPFLLVTWSSAAFIISYVVAVLSGHVNPFLPYISDTGTTPPESGIFGFMINFSAFLGAAT MYTRYKIVEKQNETCYFSTPVFNLVSLALGLVGCIGMGIVANFQELAVPVVHDGGALLAFVCGVVYTLLQ SIISYKSCPQWNSLTTCHVRMAISAVSCAAVVPMIACASLISITKLEWNPKEKDYIYHVVSAICEWTVAF GFIFYFLTFIQDFQSVTLRISTEINDDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027878 |
ORF Size | 714 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027878.2, NP_082154.2 |
RefSeq Size | 2807 bp |
RefSeq ORF | 717 bp |
Locus ID | 71712 |
UniProt ID | Q9DC58 |
MW | 26.7 kDa |
Gene Summary | Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux
,Beaumatin, F;O'Prey, J;Barthet, VJA;Zunino, B;Parvy, JP;Bachmann, AM;O'Prey, M;Kania, E;Gonzalez, PS;Macintosh, R;Lao, LY;Nixon, C;Lopez, J;Long, JS;Tait, SWG;Ryan, KM;,
Mol. Cell
,PubMed ID 31492633
[DRAM1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211216 | Dram1 (untagged) - Mouse DNA-damage regulated autophagy modulator 1 (Dram1), (10ug) |
CNY 3,990.00 |
|
MG220640 | Dram1 (tGFP-tagged) - Mouse DNA-damage regulated autophagy modulator 1 (Dram1), (10ug) |
CNY 2,850.00 |
|
MR220640L3 | Lenti ORF clone of Dram1 (Myc-DDK-tagged) - Mouse DNA-damage regulated autophagy modulator 1 (Dram1) |
CNY 4,750.00 |
|
MR220640L4 | Lenti ORF clone of Dram1 (mGFP-tagged) - Mouse DNA-damage regulated autophagy modulator 1 (Dram1) |
CNY 4,750.00 |