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Engase (NM_172573) Mouse Tagged ORF Clone
CAT＃: MR220589
- TrueORF®
Engase (Myc-DDK-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase)
ORF Plasmid: tGFP
"NM_172573" in other vectors (4)
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CNY 5,376.00
CNY 6,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C130099A03; D230014K01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220589 representing NM_172573
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCTCGTCGGTGCTGACCCGGGGAGCTGCAAGGCAGCGGAGCCCCGCGGCCCCGGAGAAGCAGG CGCGGGACCAGACGGAGCGGAGGCCGGGGCGGCGGAGGCAGGGGCGGAGGATCAACGAGGATCAAGAGGA GGAAGCAGTCTTTAGAGAGGTGGTCAGTTTCACCCCGGATCCTTTACCAGCTAGATATTATGACAAGGAT ACTACCAGACCTATCAGCTTTTACCTGTCGACGCTGGAGGAGCTCCTGGCCTGGACCCCGCTCATGGAGG ATGGCTTCAATGTAGCCCTGGAACCCCTGGTGTGTCGCCGGCCTCCACTCAGCAGCCCCAGGCCCCGGAC CCTGCTGTGCCACGACATGATGGGTGGTTACCTGGAGGACAGGTTCATTCAGGGCTCAGAGGTGCAGAAC CCCTATTCCTTCTACCACTGGCAGTATATCGACATCTTTGTGTACTTCAGCCACCACACGGTCACCATCC CCCCAGTGTGCTGGACCAATGCCGCACACAGGCACGGGGTCTGCGTGCTGGGGACCTTCATCACAGAGTG GCAAGAGGGAGGCAGGCTCTGCGAAGCCTTCCTGGCCGGGGATGAGCCCTCCTTCCAGGCCGTGGCCGAT CGGCTGGTCCAGATTGCTCAGTTCTTTCGTTTTGACGGCTGGCTCATCAACATCGAGAACTCCCTGACTC CAGCTGCCGTGAGGAACACACCTTTATTTCTCCAGTACCTTACCGCACAGCTCCATCAGCAGGTCCCCGG AGGTCTGGTGCTGTGGTACGACAGTGTGGTACAGAGCGGGCAGCTCAAGTGGCAGGATGAACTGAATGAT CAGAACCGGGTCTTCTTTGACTCCTGCGATGGCTTCTTTACCAACTATAACTGGCGGGAGGATCACCTGC AGCGGATGGTGGCACAGGCCGGGGAGCGCCTGGCTGACGTGTACGTGGGAGTGGATGTGTTTGCTCGGAG CAATGTGGTCGGAGGCCGTTTTGATACTGACAAGTCCCTGGAGCTGATCCGCAAACATGGCTTCTCGGCG GCGCTGTTTGCTCCTGGGTGGGTGTATGAGTGTCTGGAGAAGAGCGACTTCTTTCAGAACCAGGACAAGT TCTGGAGCCTGCTGGAGCGCTTCCTTCCCACACACAGTATCTGCTCCCTGCCCTTCGTCACCTCTTTCTG CCTGGGCCTGGGTACTCGAAGAGTCTGCTACGGCAAGGAGCAAGCAGTGGGGCCCTGGTACCACCCTAGC GCCCAGGAGACACAGCCCCTCTTTGGTGAACATAAGCTGGCTGGTGACAGTCGGGGCTGGGTGAAGACGC ACTGCTGCCTGACTGATGCCTGGCACGGGGGCAGCTCTCTGCTGCTCCGGGGCCTGATCCCGCCTGAGGT TGACAGTGTAGCTGTGAGGCTGTTCTCTCTGCACATCCCAGTGCCACCCAAGGTTTTCCTGTCCATGGTG TACAAGTTTGAAGGCTCAACAGATGTCCAGGTGGCTTTGGAGCTCACAACAGGGGATGCCAGCAGCTGTC ATGTGGGTGGCATGTTGGTGTTAAATGAAACTGGCTCACGGCACAGCCCCAGACCCCTCCGGGTACCCCC CACCAGGCTGGCCAGATGGGCCAGCAGTTGTGGCCAGCAGCTCAGTGGGGGCTGGATTCAACGCTGCTAT GAGGTAAACCTTCGTGGGTGCCTGCTCCAGGACCTCTTGGTGAGCTTCTCACGGCCGCCAGGCAGCCGGG AGGAGGAGAGCTTCATTTGTCGCCTTGGAGAGATCCAGGTGGTTGATGCCAGCAGCCTTCTGGCCCCTCT GCCCCGGGTGCAGAATGTCACCATTTCTCAGATACGATGGCTTCCTCTGATCACCGGGTCTGAGGGCCTC CCTACCCGGCTCCTCCTCAGCTGCACCCTGCACTGGTCCTACCTGCTGCTCCGAGCCCGCTGCTTCCGCA TTCACTGCTGGAAACGGACAGGAAGCAGCAGCTCTGTGGCAGAGTCCCCAGAGACAGAGAAGCCCACGTT CCTGGGCCTGGCTTTCGCCAACCAGTACCGTGTGGTGGACTTGGCTGTGGAGGCTGCTGGATTTGGGCAG GATGGTCGTGTGGAGTTCCTGGTGGAGCCTGTCCCCAGGGAGGGTTTCCTGGTGCCCCAGGCTGAGTGGG GAAAGGCGGTCCTGCTCTTCTCGGTCCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220589 representing NM_172573
Red=Cloning site Green=Tags(s) METSSVLTRGAARQRSPAAPEKQARDQTERRPGRRRQGRRINEDQEEEAVFREVVSFTPDPLPARYYDKD TTRPISFYLSTLEELLAWTPLMEDGFNVALEPLVCRRPPLSSPRPRTLLCHDMMGGYLEDRFIQGSEVQN PYSFYHWQYIDIFVYFSHHTVTIPPVCWTNAAHRHGVCVLGTFITEWQEGGRLCEAFLAGDEPSFQAVAD RLVQIAQFFRFDGWLINIENSLTPAAVRNTPLFLQYLTAQLHQQVPGGLVLWYDSVVQSGQLKWQDELND QNRVFFDSCDGFFTNYNWREDHLQRMVAQAGERLADVYVGVDVFARSNVVGGRFDTDKSLELIRKHGFSA ALFAPGWVYECLEKSDFFQNQDKFWSLLERFLPTHSICSLPFVTSFCLGLGTRRVCYGKEQAVGPWYHPS AQETQPLFGEHKLAGDSRGWVKTHCCLTDAWHGGSSLLLRGLIPPEVDSVAVRLFSLHIPVPPKVFLSMV YKFEGSTDVQVALELTTGDASSCHVGGMLVLNETGSRHSPRPLRVPPTRLARWASSCGQQLSGGWIQRCY EVNLRGCLLQDLLVSFSRPPGSREEESFICRLGEIQVVDASSLLAPLPRVQNVTISQIRWLPLITGSEGL PTRLLLSCTLHWSYLLLRARCFRIHCWKRTGSSSSVAESPETEKPTFLGLAFANQYRVVDLAVEAAGFGQ DGRVEFLVEPVPREGFLVPQAEWGKAVLLFSVPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172573 |
| ORF Size | 2202 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172573.3 |
| RefSeq Size | 3922 bp |
| RefSeq ORF | 2205 bp |
| Locus ID | 217364 |
| UniProt ID | Q8BX80 |
| MW | 82.9 kDa |
| Gene Summary | Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221196 | Engase (untagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase), (10ug) |
CNY 9,500.00 |
|
| MG220589 | Engase (tGFP-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase), (10ug) |
CNY 7,130.00 |
|
| MR220589L3 | Lenti ORF clone of Engase (Myc-DDK-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase) |
CNY 8,360.00 |
|
| MR220589L4 | Lenti ORF clone of Engase (mGFP-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase) |
CNY 8,360.00 |
