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Rasip1 (NM_028544) Mouse Tagged ORF Clone
CAT＃: MR216857
- TrueORF®
Rasip1 (Myc-DDK-tagged) - Mouse Ras interacting protein 1 (Rasip1)
ORF Plasmid: tGFP
"NM_028544" in other vectors (4)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110025D03Rik; 2610025P08Rik; AI853551; Rain |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216857 representing NM_028544
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTATCTGGTGAACGAAAGGAGGGCGGAAGCCCCCGCTTCGGGAAGCTCCATCTTCCAGTGGGCCTGT GGATCAATTCCCCAAGGAAACAGCTCGCCAAACTGGGGCGGCGCTGGCCCAGCGCTGCCTCTGTCAAGTC CTCGTCCTCCGACACGGGGAGCCGAAGCAGCGAGCCCCTGCCGCCGCCGCCGCCGCCGCCGCACGTGGAG CTGCGGCGCGTAGGTGCGGTGAAGGCGGCGGGGGGAGCGTCGGGCAGCCGAGCCAAGCGCATCTCGCAGC TGTTTCGGGGCTCAGGGGCCGGCGGCGCCGGGGGTCCTGGCACGCCTGGGGGCGCGCAGCGCTGGGCCAG CGAGAAGAAGCTGCCTGAACTGGCGGCGGGTGTAGCTCCGGAGCCCCCGTTGCCCACGCGTGCCGCGGTG CCCCCGGGAGTGCTCAAGATCTTCGCTTCTGGGCTGGCGTCGGGCGCCAACTACAAGAGCGTGCTGGCTA CGGAGCGATCCACCGCGCGCGAGCTGGTGGCGGAGGCGCTGGAGCGCTACGGGCTGACGGGCGGCCGCGG AGCGGGCGACAGCGGCTGCGTGGACGCCTACGCGCTTTGCGACGCTCTGGGCCGGCCCGCGGTCGGCGTG GGCGGAGGCGAGTGGCGTGCCGAGCACCTGCGGGTGTTGGCCGACGCGGAGCGCCCTCTGCTGGTGCAGG ACCTGTGGCGCGCCCGTCCGGGCTGGGCGCGGCGCTTTGAGCTGCGCGGCCGCGAGGAGGCTCGACGGCT GGAGCAGGAGGCCTTCGGGGCTGCGGATGCAGACGGCACAAACGCCCCATCTTGGCGGACCCAGAAGAAT AGGTCTCGGGCAGCATCTGGCGGGGCAGCTCTGGCCAGTCCTGGTCCAGGATCAGGATCCGGGACCCCCA CTGGGTCCGGGGGCAAGGAGCGATCTGAAAATTTGTCTCTGCGTCGAAGCGTGTCTGAGCTGAGTCTACA GGGGCGGAGGCGAAGGCAGCAGGAACGCAGGCAGCAGGCCCTTAGCATGGCCCCAGGGGCAGCTGATGCT CAGATGGTACCAACGGACCCTGGAGACTTCGATCAGTTGACTCAGTGCCTCATTCAGGCACCCAGCAACC GTCCCTACTTCCTTCTGCTCCAGGGCTACCAGGATGCTCAGGACTTCGTGGTGTATGTCATGACACGAGA GCAGCACGTGTTCGGCCGGGGTGGGCCCTCCTCGTCCCGCGGGGGATCCCCAGCCCCATATGTGGACACC TTCCTCAACGCCCCAGACATCCTGCCTCGACATTGCACGGTGCGCGCGGGCCCTGAGCCCCCGGCCATGG TGCGCCCATCCCGGGGCGCCCCCGTCACACACAATGGCTGTCTTTTGCTGCGGGAGGCAGAGCTGCACCC CGGAGACCTGTTAGGCCTAGGAGAGCATTTCCTGTTCATGTACAAGGACCCCCGCTCCGGGGGTTCTGGG CCCGCTCGGCCATCTTGGCTGCCAGCGCGCCCCGGGGCCGCGCCACCCGGACCGGGCTGGGCCTTTTCTT GTCGTTTGTGTGGCCGTGGCCTGCAGGAGCGCGGCGAGGCGCTGGCCGCCTACCTGGATGGCCGCGAGCC GGTGCTGCGGTTCAGACCACGAGAGGAAGAGGCGCTGTTGGGCGAGATCGTGCGAGCTGCAGCCTCGGGA GCAGGGGACCTGCCCCCGCTCGGGCCAGCCACTCTGCTGGCACTGTGTGTGCAGCATTCTGCGAGGGAGC TGGAGCTGGGCCACCTGCCTCGCTTGCTGGGCCGCCTGGCCCGGCTCATCAAAGAAGCCGTTTGGGAAAA AATTAAAGAAATTGGAGACCGCCAGCCAGAGAACCACCCAGAGGGAGTTCCCGAGGTGCCTCTGACCCCT GAGGCTGTGTCTGTGGAGCTGCGACCACTCATACTATGGATGGCCAACACCACAGAGCTGTTGAGCTTTG TACAGGAGAAAGTGCTAGAGATGGAGAAGGAGGCTGACCAGGAGGGCCTGTCCTCGGACCCACAGCTCTG CAATGACTTGGAACTGTGTGATGAGGCCTTGGCCCTCCTGGATGAGGTCATCATGTGTACTTTCCAGCAG TCGGTCTACTACCTCACCAAGACTCTTTATTCAACACTGCCAGCTCTCCTGGACAGTAACCCTTTCACAG CTGGAGCAGAGCTGCCAGGGCCTGGCGCAGAGCTGGAGGCCATGCCGCCTGGGCTCAGGCCTACTCTGGG TGTGTTCCAGGCAGCTCTAGAACTGACCAGTCAGTGTGAGCTGCATCCCGACCTCGTGTCCCAGACTTTT GGCTATTTGTTCTTCTTCTCCAATGCATCCCTTCTCAACTCACTGATGGAACGAGGTCAAGGCCGACCTT TCTATCAATGGTCTCGAGCCGTCCAAATCAGAACCAACTTGGATCTTGTGTTGGATTGGCTTCAGGGGGC CGGGCTGGGTGACATTGCCACTGAGTTCTTCCGGAAACTTTCCATTGCTGTGAACCTGCTATGTGTGCCC CGCACCTCCCTGCTCAAGGCTTCATGGAGCAGCTTACGGACTGACTACCCCACCCTGACTCCAGCTCAGC TCCACCATCTCCTCAGCCACTACCAGTTGGGTCCTGGCCGTGGTCCCCCACCAGCCTGGGACCCACCACC TGCAGAGCGGGATGCTGTGGACACAGGGGATATCTTTGAAAGTTTTTCCTCGCACCCTCCGCTCATCCTG CCCCTGGGCAGCTCACGCCTGCGCCTCACCGGCCCTGTGACAGATGACGCATTGCATAGAGAACTGCGCA GGCTCCGTCGCCTCCTCTGGGATCTTGAACAGCAGGAGCTGCCAGCCAATCATCGCCATGGACCCCCGGT GGCTTCGACACCT CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC >MR216857 representing NM_028544
Red=Cloning site Green=Tags(s) MLSGERKEGGSPRFGKLHLPVGLWINSPRKQLAKLGRRWPSAASVKSSSSDTGSRSSEPLPPPPPPPHVE LRRVGAVKAAGGASGSRAKRISQLFRGSGAGGAGGPGTPGGAQRWASEKKLPELAAGVAPEPPLPTRAAV PPGVLKIFASGLASGANYKSVLATERSTARELVAEALERYGLTGGRGAGDSGCVDAYALCDALGRPAVGV GGGEWRAEHLRVLADAERPLLVQDLWRARPGWARRFELRGREEARRLEQEAFGAADADGTNAPSWRTQKN RSRAASGGAALASPGPGSGSGTPTGSGGKERSENLSLRRSVSELSLQGRRRRQQERRQQALSMAPGAADA QMVPTDPGDFDQLTQCLIQAPSNRPYFLLLQGYQDAQDFVVYVMTREQHVFGRGGPSSSRGGSPAPYVDT FLNAPDILPRHCTVRAGPEPPAMVRPSRGAPVTHNGCLLLREAELHPGDLLGLGEHFLFMYKDPRSGGSG PARPSWLPARPGAAPPGPGWAFSCRLCGRGLQERGEALAAYLDGREPVLRFRPREEEALLGEIVRAAASG AGDLPPLGPATLLALCVQHSARELELGHLPRLLGRLARLIKEAVWEKIKEIGDRQPENHPEGVPEVPLTP EAVSVELRPLILWMANTTELLSFVQEKVLEMEKEADQEGLSSDPQLCNDLELCDEALALLDEVIMCTFQQ SVYYLTKTLYSTLPALLDSNPFTAGAELPGPGAELEAMPPGLRPTLGVFQAALELTSQCELHPDLVSQTF GYLFFFSNASLLNSLMERGQGRPFYQWSRAVQIRTNLDLVLDWLQGAGLGDIATEFFRKLSIAVNLLCVP RTSLLKASWSSLRTDYPTLTPAQLHHLLSHYQLGPGRGPPPAWDPPPAERDAVDTGDIFESFSSHPPLIL PLGSSRLRLTGPVTDDALHRELRRLRRLLWDLEQQELPANHRHGPPVASTP NNNNEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-HindIII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028544 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028544.1, NP_082820.1 |
| RefSeq Size | 3169 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 69903 |
| UniProt ID | Q3U0S6 |
| MW | 104 kDa |
| Gene Summary | Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222836 | Rasip1 (untagged) - Mouse Ras interacting protein 1 (Rasip1), (10ug) |
CNY 12,260.00 |
|
| MG216857 | Rasip1 (tGFP-tagged) - Mouse Ras interacting protein 1 (Rasip1), (10ug) |
CNY 10,550.00 |
|
| MR216857L3 | Lenti ORF clone of Rasip1 (Myc-DDK-tagged) - Mouse Ras interacting protein 1 (Rasip1) |
CNY 11,500.00 |
|
| MR216857L4 | Lenti ORF clone of Rasip1 (mGFP-tagged) - Mouse Ras interacting protein 1 (Rasip1) |
CNY 11,500.00 |
