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Pde4a (NM_183408) Mouse Tagged ORF Clone
CAT＃: MR216607
- TrueORF®
Pde4a (Myc-DDK-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5
ORF Plasmid: tGFP
"NM_183408" in other vectors (4)
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CNY 6,192.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D9Ertd60e; Dpde2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216607 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTCCGGCCGCCCCCTCGGAGAGGAGCCTGTCTCTCTCTCTTCCCGGGCCCCGAGAGGGCCAGG CCACCCTGAAGCCGCCCCCCCAGCACCTGTGGCGGCAGCCGAGGACCCCGATCCGCATCCAGCAGCGCGG CTACTCGGACAGTGCCGAGCGCTCAGAGCCAGAGCGCTCGCCGCACCGGCCCATAGAGCGCGCCGACGCC GTGGACACTGGCGACCGGCCAGGCCTGCGGACTACCCGCATGTCCTGGCCCTCGTCCTTCCACGGCACCG GTACCGGCGGAGGCAGCAGTAGGCGCTTGGAGGCAGAAAATGGGCCAACACCGTCCCCTGGCCGCAGCCC CCTGGACTCGCAGGCGAGCCCGGGGCTTATGCTGCATGCCGGGGCAGCCACCAGCCAGCGCCGAGAATCC TTCCTCTATCGCTCAGACAGCGACTATGACATGTCACCGAAGACTATGTCCAGGAACTCATCCGTCGCCA GCGAAGCGCATGGTGAAGACCTCATTGTGACCCCGTTTGCCCAGGTGCTGGCCAGTCTCCGCAACGTTCG AAGCAACTTCTCACTCCTAACCAATGTGCCCATCCCCAGCAACAAGAGGTCCCCGCTGGGCGGGCCACCC TCTGTCTGCAAGGCCACACTGTCAGAGGAGACATGCCAGCAGCTGGCCCGGGAGACCCTGGAAGAGCTGG ATTGGTGCCTGGAGCAGCTGGAGACCATGCAGACCTACCGCTCTGTCAGCGAGATGGCCTCACACAAGTT TAAAAGGATGCTGAACCGGGAACTCACACACCTGTCGGAAATGAGCAGGTCAGGAAACCAGGTCTCAGAG TACATTTCCAACACGTTCCTAGACAAGCAGCACGAAGTGGAGATCCCATCGCCCACGCCGCGACAGAGAC CCTTCCAGCAGCCCCCACCAGCAGCAGTGCAGCAGGCCCAGCCCATGTCTCAGATCACAGGGCTGAAAAA GCTGGTACACACCGGAAGCTTGAACATCAATGTCCCACGATTTGGAGTCAAGACAGATCAGGAGGACCTC TTAGCACAAGAACTGGAGAACTTGAGCAAATGGGGCCTGAACATCTTTTGTGTGTCGGAGTACGCTGGAG GCCGTTCACTCAGCTGTATCATGTATACGATATTCCAGGAGCGGGACTTACTGAAGAAATTCCACATCCC CGTGGACACCATGATGACATACATGCTGACGCTGGAGGACCACTACCACGCTGACGTGGCCTACCACAAC AGCCTGCACGCAGCCGATGTGCTACAGTCCACACATGTGCTGCTGGCCACGCCTGCACTGGATGCCGTGT TCACAGACCTGGAGATTCTTGCTGCTCTCTTCGCTGCTGCCATCCATGACGTGGACCACCCTGGCGTCTC CAACCAGTTCCTAATCAACACCAATTCGGAGCTGGCATTGATGTACAACGATGAGTCTGTGCTTGAGAAC CACCACCTGGCTGTGGGATTCAAGCTGCTGCAAGAAGAGAACTGCGACATCTTCCAGAACCTCAGCAAGC GCCAGCGGCAGAGCCTGCGCAAGATGGTCATCGACATGGTGCTGGCCACAGACATGTCCAAGCACATGAC CCTTCTGGCTGACCTGAAGACTATGGTAGAGACGAAGAAAGTGACCAGCTCTGGAGTTCTCTTGCTGGAC AACTACTCTGACCGTATCCAGGTCCTCAGGAACATGGTGCACTGTGCAGACCTCAGCAATCCCACCAAGC CCCTGGAGCTGTACCGACAATGGACTGATCGCATCATGGCTGAGTTCTTCCAGCAGGGTGACCGAGAACG GGAACGCGGAATGGAGATTAGCCCCATGTGTGACAAGCACACAGCCTCTGTGGAGAAGTCTCAGGTGGGC TTCATCGACTATATTGTTCACCCACTGTGGGAGACGTGGGCCGATCTCGTCCACCCCGATGCCCAAGACA TCCTGGACACGCTGGAAGACAACCGGGATTGGTATCACAGTGCCATCCGCCAGAGTCCCTCCCCAACCCT GGAAGAGGAGCCAGGAGTGCTCAGCGATCCGGCCCTGCCTGACAAGTTCCAATTTGAGCTCACCTTGGAG GAAGAGGATGAAGAGGATTCATTGGAGGTTCCAGGATTGCCTTGCACTGAGGAAACTCTCCTGGCTCCAC ATGATACCAGAGCTCAAGCCATGGAACAGTCAAAGGTCAAAGGCCAGAGCCCTGCTGTTGTGGAGGTAGC AGAGAGTTTGAAGCAGGAGACAGCCTCAGCACATGGCGCTCCTGAGGAGTCCGCGGAGGCTGTGGGCCAT TCCTTCAGCCTTGAAACCTCTATTCTGCCTGACTTGAGGACCCTGTCCCCCTCAGAGGAGGCCCAGGGCC TCCTGGGCCTCCCCTCCATGGCGGCAGAGGTGGAGGCCCCAAGAGACCATCTGGCTGCCATGAGGGCTTG TTCTGCCTGCTCGGGGACATCAGGAGACAATTCTGCCGTCATCTCTGCTCCAGGCAGGTGGGGGTCAGGT GGAGACCCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216607 protein sequence
Red=Cloning site Green=Tags(s) MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERSEPERSPHRPIERADA VDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLMLHAGAATSQRRES FLYRSDSDYDMSPKTMSRNSSVASEAHGEDLIVTPFAQVLASLRNVRSNFSLLTNVPIPSNKRSPLGGPP SVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSE YISNTFLDKQHEVEIPSPTPRQRPFQQPPPAAVQQAQPMSQITGLKKLVHTGSLNINVPRFGVKTDQEDL LAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMTYMLTLEDHYHADVAYHN SLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLEN HHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLD NYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVG FIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPTLEEEPGVLSDPALPDKFQFELTLE EEDEEDSLEVPGLPCTEETLLAPHDTRAQAMEQSKVKGQSPAVVEVAESLKQETASAHGAPEESAEAVGH SFSLETSILPDLRTLSPSEEAQGLLGLPSMAAEVEAPRDHLAAMRACSACSGTSGDNSAVISAPGRWGSG GDPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183408 |
| ORF Size | 2535 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183408.3, NP_899668.1 |
| RefSeq Size | 4658 bp |
| RefSeq ORF | 2535 bp |
| Locus ID | 18577 |
| UniProt ID | O89084 |
| MW | 93.6 kDa |
| Gene Summary | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221997 | Pde4a (untagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5, (10ug) |
CNY 10,740.00 |
|
| MG216607 | Pde4a (tGFP-tagged) - Mouse phosphodiesterase 4A cAMP specific (Pde4a) transcript variant 5, (10ug) |
CNY 7,890.00 |
|
| MR216607L3 | Lenti ORF clone of Pde4a (Myc-DDK-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5 |
CNY 9,030.00 |
|
| MR216607L4 | Lenti ORF clone of Pde4a (mGFP-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5 |
CNY 9,030.00 |
