Ssc5d (NM_173008) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A430110N23Rik; S5D-SRCRB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR214569 representing NM_173008 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGCTTGGCCTGCCTCCTTGCAATGCTGGTGGGGATCCAGGCTATAGAGCGACTCCGCCTGGCTG ATGGTCCTCATGGCTGCGCAGGACGCCTGGAGGTCTGGCACAGCGGGCGCTGGGGCACCGTGTGTGATGA CGGATGGGACCTTCGGGATGCTGAAGTGGCCTGCCGTGTGTTAGGCTGCGGAGGGGCGCTGGCAGCCCCT GGGGGTGCCTTCTTCGGGGAGGGCACTGGGCCTGTATGGCTCAGTGAACTGAACTGTCGGGGCAATGAGG GTCAGCTGGGTATCTGTCCCCACCGGGGCTGGAAGGCCCATATCTGCTCTCATGAGGAGGACGCAGGCGT CGTCTGTGTAGGTCAGCGTGCAGCTAACTCTAGAGAAGATTCAATGTCCCTGCTGGATGGGGATCCATGG CTGGCACTGTCTGGGGAGCTAAGCCCCAGCTCAGAGGAGCCCCCTATAACTCACGCTCCCCAACCAGCAG CAAGCTCCCAGAATGGCCCCCGGAAGAAGAACCCCCGGCCACCCAAGCAGACCAAGTCCACCCGAGCCCC TGTACTGACAAATGGAGCTCCCCACCAAGAGCGGCTGCGACTGGTCTCAGGTCCCCACGGATGTGCCGGC CGCCTGGAAGTCTGGCACGGTGGACGTTGGGGCACTGTCTGTGATGATGGATGGGACCTCCGAGATGCAG CGGTGGCCTGCAGGGAGCTGGGATGTGGGGGAGCGCTGGCCGCCCCTGGGGGTGCCAGATTTGGACCTGG TGAGGGACCTGTGTGGATGGATGACGTGGGGTGTGGAGGAGGAGAGGAGGCCCTCCGAGACTGTCCCCGA AGCCCCTGGGGCCGGAGCAACTGTGACCACACCGAGGATGCAGGGCTGGTCTGCACCGGTCCTGCACCCA GGATACGCCTTGCTGATGGTCCCCACGGCTGTGCTGGCCGCCTGGAGGTGTGGCATGGTGGACGATGGGG GTCGGTGTGTGACGATGCCTGGGATCTTCGCGATGCTGCTGTGGCCTGCAAGGAGCTGGGCTGTGGGGGT GCCCTGGCCGCCCCAGGGGGCGCCTTCTTTGGAGAGGGGACTGGACCCATCATCCTGGATGATCTCAGAT GTCGAGGAAATGAGACAGCCTTGCGATTCTGTCCTGCGAGGCCCTGGGGTCAGCACGACTGTCACCACCG GGAGGATGCTGGGGCCGTGTGTGATGGCATGCCTCTCGGTGCTGTGCAGCCTACAGTTCCTGCAGTGGAC AGCAACAGCACAGCACACAGGCTTCTGTCTACCTCCGTGGGTCAGATGCCAGGCCCAGCAGGCCCTTGGC CTCCTTCTGCTTCTCCTACTGCCCCTCCAGAGCCTGGGCCCGAAGCTGGGTCCCCCCAGTTGCGCCTGGT GGCTGGACCCAGCAGGTGCTCTGGCCGGCTGGAGGTATGGCATGACGGACGCTGGGGGACAGTATGTGAT GACAGCTGGGACATGAGAGACTCAGCTGTGGTCTGCCGGGAGCTGGGCTGTGGGAGGCCTCGGCAACCAG ACCCCGCAGCAGGCCGCTTTGGCTGGGGTGCAGGCCCCATCTGGCTGGATGACGTAGGTTGTATGGGGAC GGAGGCTTCACTGTCAGAATGCCCTGCTGCTTCCTGGGGGAAACACAATTGCGCCCACAATGAGGATGTC GGGGTTACCTGCACTGGGACCCCCGGCCTGGATACCATCTCAGACCCTTTCAGTTGGAGCTGGCTCCCTG GGCTGGGTAGAGATCAGGATGCCTGGCTCCCAGGAGAGCTAACCACCAAACCTTCTGCCAGTCTCACCTC CAGTGTGCCACAGAAACCCACAAAGGTTCCAGGGAAAGCTCCCAAGAGTACCAAGAAGTGGGTGACTAAA AATGCAAGGAGACCAACCACTCAGCCCCCTGGGATGCCAACCACCAAACATTCCAGGGCCCCAGGCACCC CAACCTCCCTACATCCAACAGCACGTACCTCTGAGTTACCAAAGAGACTGACCACTGAGGCTCCCCACAG GCAGACCTCACATACCACTGTAAGGCTGACCCCTAGGGTCCCCTGGGAGTGGACCTCAGAGCCTGTGGTG TCACAGTCCACTCAAGGTCCCCAAGAAGTGACCTCTGAGGCCACCACAACGGAAAACCCTCAGACCTCTT TGGAGCCATCGGGTGAGAACACCGAAGGCTCTCTGGAATCATCCCAAGATCCAGCTACCACCCCAACTGC TGGAGTCCCTGTGCCATCAGGTCCCTTCCGGGTTCGTCTGGCTGATGGGCCCAACAGGTGTGCTGGCCGG CTAGAGGTGTGGCATGCTGGACTCTGGGGAACAGTCTGTGATGACAGCTGGGACATCCGGGATGCCACAG TGGCCTGCTGGGAGCTGGGTTGCGGAAAGGTCCGGCCCCGAGTAGGCAAAACTCACTATGGCCCTGGCAC TGGGCCCATCTGGTTGGATGACATGGGCTGTAAAGGAAGTGAGATGTCACTGAGTGACTGCCCCTCGGGG GCATGGGGGAAGCACAACTGTGACCACGAAGAAGACGTGGTGCTCACCTGCACTGGCTACACGGGTGATG ACGATTATCCTTCCTGGACCTGGGACCCTACTTCCGGAGAGGACCTGACCAAAGGGACCACAGTGGCTGC GCGGCCTGGACATACACTTTCCTGGGCTACCACTACAAACACTGAAGTCCCCTCTCCAGCAACACAGAAC CTTCCAGACACGGATGACCAGGGAGGTTATGAGTCTTCCTGGACGTGGGATACACCTTCAGGAAGGGGTC TGTTCAAGGGGACCCCCACCACAACCAAGCCTGGATCCACAGTTACCACTAGCACCAGCAAGAGTCCCGG CCACCCCTTTCCAGCTCCAAGGGCCCGTGCAGGTTCACCAAGGAAGCCAACACCTGAGCGCCGGCCGCTG CCCACCTCAGCCACCACCTCATCTCCTGCTTCCTCCTCTTCACCAGAGCCTTCTGGCTCACGGCAGACCT CTGGCTCCTGGCCACAACTCATCCCAGACTCAAAGCAGGAGGGGACCTCTAGCTCCCCCAAACCATCTCT ACTCACCCCAGGACTTCCCTCCCCAGCAACCTTTGCCTTGTCCACTCCTAATACCAGTTTGCTTCCAACA CGTTCCCCAGAGCTTTCAGGCAGCCCAACACCCACCTCCCCTGAAGGGCTAACCTCTGCCTCCTCTATGC TATCAGAAGTGAGCAGGCTTTCTCCTACCTCAGAGCTGACTCCAGGACCTGATACAACACCAGCACCTGA AATAATCCCAGAAAGTTCTGACTCCTCAGACCTCCCCATGAACACCAGGACCCCCACACAGCCGTTTACA GCCTCTCACCCCACCAGCATCCCTCAACTCAACACAACTTCTTACCCCACCATTGCCCCTCAACCTACCA CAAACCCTCAGCAACCCAGGAGCCCTCACCCCGCCACAAGTCCTCAACCTCCCACCAATACTCACCCTTC CTCAACCCCTGCCACCCCTACAGAGTCCCTTCCATCCTCCCGAAAGACAGAACTTTCCTCTCCCACTAAG CCTAGACTAAACTCAGAGTTGACCTTTGAGGAAGCCCCATCCACAGACGCATCCCAGACGCAGAACCTAG AGCTCTTTCTAGCCTCGGAGTCCGGCCCCTCCAGTCCCTCTCCAGCCTCAAACTTGGACCCACTACCCAC AGATGCCTTCAAACCACCCAGAAGCCAGACCTTACACTCAGCTTCAGACCACCTCACTCAAGGGCCAACC CCTAATCACAACCCAGATCCTTTTGGGCCATGTGTGTCCCCATTACCACCAGTAAGGGTCATGGCTTGTG AGCCACCTGCCTTGGTGGAGCTGGTAGGTGCTGTGAGGGAGGTGGGTGACCAGCTGCAGAGACTGACCTG GGTCCTGGAGCAGGACCGGCAGGAGCGCCAAGTCCTGGCACTGGGCCTAGCCCAGTTGGTAGAGGCTGCT CAGGGGTTGGGGCAGCTGAGTGAGACTGTAAAGAGGCTAGCAGAGGTGGCCTGGCCCCCCAGCACTCCTG TGCCCATGACCACCACCACAGAGGAGGAGGAGAGGCCTCTGAGGGGAGATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR214569 representing NM_173008 Red=Cloning site Green=Tags(s) MRGLACLLAMLVGIQAIERLRLADGPHGCAGRLEVWHSGRWGTVCDDGWDLRDAEVACRVLGCGGALAAP GGAFFGEGTGPVWLSELNCRGNEGQLGICPHRGWKAHICSHEEDAGVVCVGQRAANSREDSMSLLDGDPW LALSGELSPSSEEPPITHAPQPAASSQNGPRKKNPRPPKQTKSTRAPVLTNGAPHQERLRLVSGPHGCAG RLEVWHGGRWGTVCDDGWDLRDAAVACRELGCGGALAAPGGARFGPGEGPVWMDDVGCGGGEEALRDCPR SPWGRSNCDHTEDAGLVCTGPAPRIRLADGPHGCAGRLEVWHGGRWGSVCDDAWDLRDAAVACKELGCGG ALAAPGGAFFGEGTGPIILDDLRCRGNETALRFCPARPWGQHDCHHREDAGAVCDGMPLGAVQPTVPAVD SNSTAHRLLSTSVGQMPGPAGPWPPSASPTAPPEPGPEAGSPQLRLVAGPSRCSGRLEVWHDGRWGTVCD DSWDMRDSAVVCRELGCGRPRQPDPAAGRFGWGAGPIWLDDVGCMGTEASLSECPAASWGKHNCAHNEDV GVTCTGTPGLDTISDPFSWSWLPGLGRDQDAWLPGELTTKPSASLTSSVPQKPTKVPGKAPKSTKKWVTK NARRPTTQPPGMPTTKHSRAPGTPTSLHPTARTSELPKRLTTEAPHRQTSHTTVRLTPRVPWEWTSEPVV SQSTQGPQEVTSEATTTENPQTSLEPSGENTEGSLESSQDPATTPTAGVPVPSGPFRVRLADGPNRCAGR LEVWHAGLWGTVCDDSWDIRDATVACWELGCGKVRPRVGKTHYGPGTGPIWLDDMGCKGSEMSLSDCPSG AWGKHNCDHEEDVVLTCTGYTGDDDYPSWTWDPTSGEDLTKGTTVAARPGHTLSWATTTNTEVPSPATQN LPDTDDQGGYESSWTWDTPSGRGLFKGTPTTTKPGSTVTTSTSKSPGHPFPAPRARAGSPRKPTPERRPL PTSATTSSPASSSSPEPSGSRQTSGSWPQLIPDSKQEGTSSSPKPSLLTPGLPSPATFALSTPNTSLLPT RSPELSGSPTPTSPEGLTSASSMLSEVSRLSPTSELTPGPDTTPAPEIIPESSDSSDLPMNTRTPTQPFT ASHPTSIPQLNTTSYPTIAPQPTTNPQQPRSPHPATSPQPPTNTHPSSTPATPTESLPSSRKTELSSPTK PRLNSELTFEEAPSTDASQTQNLELFLASESGPSSPSPASNLDPLPTDAFKPPRSQTLHSASDHLTQGPT PNHNPDPFGPCVSPLPPVRVMACEPPALVELVGAVREVGDQLQRLTWVLEQDRQERQVLALGLAQLVEAA QGLGQLSETVKRLAEVAWPPSTPVPMTTTTEEEERPLRGDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_173008
ORF Size	4113 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_173008.3
RefSeq Size	4260 bp
RefSeq ORF	4116 bp
Locus ID	269855
UniProt ID	Q8BV57
MW	144.6 kDa
Gene Summary	Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces.[UniProtKB/Swiss-Prot Function]

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