Sec16a (NM_153125) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU024582; C230052J16Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR213071 representing NM_153125 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCACCACCTCAGGCAGTCCCGTCTGGGGTGGCTGGACCACCCCCAGCTGGGAATCCTCGGAGCA TGTTCTGGGCCAACAGCCCTTACAGGAAGCCAGCCAATAATGCACCAGTGGCTCCCATAACACGCCCATT GCAGCCAGTAACGGATCCGTTTGCTTTTAATAGACAGACTCTTCAAAACACACCAGTAGGAAGTTCATCC AAAAGCAGCCTGCCCAATTTGCCTGGCCCAGCCCTCTCTGTTTTTTCTCAGTGGCCTGGTTTGCCTGTGA CTCCCACAAATGCTGGGGATAGCTCCACAGGATTGCATGAGCCTCTGTCAGGAACTCTGTCACAGCCTAG AGCAGATGCCAGTCTATTTCCTCCTGCATCTACCCCTTCTTCACTCCCTGGGCTGGAGGTGAGCAGGAAT GCTGAGGCTGATCCCAGCTCAGGACATGAAGTCCAGATGCTGCCACATTCAGCTCACTACATTCCAGGAG TGGGTCCTGAGCAGCCTCTCGGGGGCCAAATGAATGACAGTGGGTCCGGGCCTGACCAACCCATGAATAG GCATGCCCCCCATGATGGTGCCGTGACCCACGCAGCATCTCCTTTCCTCCCTCAGCCACAGATGCCAGGT CAGTGGGGGCCTGCACAAGGAGGCCCTCAGCCCTCCTATCAGCATCACTCACCCTACCTGGAGGGACCTG TTCAGAACATGGGGCTCCAGGCTGCCAGCTTGCCCCATTTTCCCCCTCCATCTAGTCTACACCAGGGACC TGGCCATGAGTCTCATGCCCCACAAACATTTACTCCAGCATCCTTGGCCAGTGGTGAAGGGAATGAGATA GTTCACCAGCAAAGTAAAAATCACCCATTGAGTAGCTTCCCCCCTAAACACACTTTTGAGCAGAATTCCA GAATTGGGAATATGTGGGCAAGCCCAGAGTTGAAGCAGAATCCGGGAGTGAATAAAGAGCATCTGCTAGA CCCTGCCCATGTCAATCCCTTCACTCAGGGAAATAGTCCTGAAAACCAGGCGCATCATCCCCCAGTGGCA GCAACTAACCATGCCCTGCAAGAAGCAGCCTCTGGAGCTCTCTCAATGTTTTTTCAAGGGGAAGAGACAG AGAATGAGGAAAATCTCTCATCTGAAAAAGCAGGCCTCGATAAGAGATTGAACTTGGATAGCTTTTCTTC TACCTCCAGACTGGGCCATCCACCACCACCTGGAGCTAGTGGTGTCTACCAGGCCTTTCCTAGAGGCCCT AGCAGTGAGGCTGCGCAGGAAGGAGATGCGCAACCTTATTTTTCGCAGTCTGTGGGCGTCCGTCTTGACA AACAGAGCACTGTGCCCCCTGCTAATGATGCATGGGGTGATGTTCCGGGGACCGGGACCCGCTGTGCCAG TGGGCCACAGTGTGAGAATGTTGAGAACTTAGAATTTGTTCAGAATCAAGAAGTTTTGCCCCGTGAGACC CTGAGTGTGGATCCCTTCCCCCTGAGTGATCAGATCCGATATGGGCCCCTTCCTGGGCCAGCTGCCTCCA GGCCTGCTACTGTGGGCCTCACCAGAGGTGGGGGGCTAAATCTTGAGGCCCCAGACACACCACTGCACCC TACACGACCTGACAGTGTGTCATCCAGCTACAGCAGCCATAGCCACAGAAGTCCTCCTGGGTCAGCCAGA CCCCAAGAATTGGTGGGCACATTCATTCAGCAAGAAGTTGGGAAACTTGAAGATGATACTTCAGGGAGTT TTTTTAAGCAAATTGATTCTTCTCCTGTTGGAGGTGAAACAGATGAGGTGACTGGGAGCCAAAATTGCTG CAGCAGTCTGTCCCAGCCTTCAACCCCAAGCCCTCCAAAACCTACAGGAGTATTCCAAACAAGTGCAAAC AGTTCTTTTGAGCCAGTAAAATCCCACTTGGTTGGAGTGAAACCAGTTGAAGCAGATCGTGCCAACATGG TGGTTGAAGTGAGAGGGACCCAATACTGTCCGAAGAAGCGCAGGGCAGCTGTTGCACCACCTGATGCTAC CTCAGGAAACCTGGAACAGCCACCAGACAACATGGAGACCCCGTGTGCACCTCAGGCCTGCCCTTTGCCT CTTAGCACCACTGGAGAGGCTGGGCAACTGGTTTCAAACACGGCAGGGACACCCCTGGATACTGTGCGCC CAGTGCCTGATAAGAGGCCCTCAGCCAGGGCCCAGGGACCTGTGAAGTGTGAGAGCCCAGCAACAACTCT GTGGGCACAAAATGAGCTGCCAGATTTTGGTGGCAATGTCCTTCTAGCCCCTGCTGCCCCTGCACTTTAT GTTCCTGTAAAACCAAAACCTTCAGAGGTTGTTCATCATCCAGAAAAGGGAATGTCTGGACAGAAGGCCT GGAAACAAGGGTCTGTGCCACCTTTGCAGAACCAAGACCCTCCTGGTGCTTCTGAGAACCTTGAGAACCC TCCCAAGGTAGGAGAAGAGGAGGCCCTTCCAGTGCAGGCAAGTTCTGGTTATGCGAGTTTGCTTTCTTCG CCACCCACTGAATCTTTGCACAATCAACCTGTCCTGATTGCTCAGCCTGATCAAAGCTATAATTTGGCTC AGCCTATTAATTTTTCTGTGTCCTTACTGAATCCTAATGAGAAGAATCAGTCCTGGGGAGATGCTGTGGT GGGGGAGAGATCCATAGTGAGCAACAACTGGGCTCTCGGTGGTGATCCTGAAGAACGTGCTGCTCTGTCT GGAGTTCCAGCCAGTGCTGTCACTGGCGCGTCTTTGCCTAGCAGTATCCCCCAGAATTGTGCCCCTCAAG GTTCGGGGTCTTCTGAAATGATTGCTAGCCAGTCTGCCAGTTGGTTGGTTCAACAGCTATCCCCTCAAAC CCCTCAAAGTCCGCATCCAAATGCAGAAAAGGGCCCTTCCGAGTTTGTTAGTAGCCCTGCTGGCAATACA AGTGTGATGCTAGTTCCACCTGCAAGCAGTACTTTGGTTCCTAACAGTAATAAGGCAAAGCACTCTAGTA ACCAGGAAGAAGCTGTTGGCGCCCTAGACTTCACATTAAATAGAACTTTGGAAAATCCTGTGAGAATGTA CAGCCCATCTCCTTCAGATGGCCCAGCTTCTCAGCAGCCTCTTCCCAATCATCCCAGACAATCTGGGCCT GGGCTGCATAACCAGGATCATTTCTACCAGCAGGTAACAAAAGATGCTCAGGACCAGCATAGACTAGAAA GAGCCCAGCCAGAGCTGGTGCCTCCTCGGCCACAGAATTCTCCCCAAGTGCCCCAAGCATCGTGTCCAGA GCCTTCAAATCCTGAAAGCCCACCCACACAAGGACAGTCTGAAAGCTTGGCCCAGCCACCAGCAAGTCCA GCTTCAGTCAACACAGGCCAGCTGCTGCCTCAGCCACCACAGGCTTCCAGTGCATCCGTTACATCTACCA ACTCCAGCCAGGCAGCTGTGCGGTCAGAGCAGCTGTGGCTACACCCACCACCTCCTAATACTTTTGGCCC AGCACCTCAAGACTTGGCATCCTACTACTATTACAGACCCCTGTATGATGCCTACCAGTCTCAGTACCCT TCACCGTACCCGTCAGATCCTGGGACAGCCTCGCTGTACTACCAGGATATGTATGGCCTGTATGAGCCTC GTTACAGACCCTATGACAGCTCAGCATCTGCTTATGCCGAGAACCACCGCTATTCTGAGCCTGAGCGGCC CAGCTCCCGTGCAAGTCACTATTCCGACCAGCTTGCTCCCAGGCAAGGATATCCTGAAGGATACTATAAT TCCAAGAGTGGATGGAGCAGTCACAGTGATTACTATGCAAATTATTACTCTGGCCAGTATGATTATGGAG ACCCCAGCCGCTGGGATCGTTACTATGGGTCTCGCCTTAGGGATCCTCGCACCTGGGACCGGAGGTACTG GTATGACTCTGAACATGACCCATACAGGAAGGACCACTATGCTTACAGTGACAGGCCTGAGAAATGTGAT GATCACTGGAGGTATGACCCTCGCTTCACTGGGAGCTTCGACGATGACGCTGAGATCCACAGGGACCCCT ATGGAGAAGAAGCAGACAGACGCAGCATCCACAGTGAGCACTCGGCACGGAGCCTGCGCAGCACTCACAG TCTGCCCAGCCGCCGCAGCAGCCTCAGCTCCCATTCACACCAGAGTCAGATTTACAGAAGCCACCATGTG ACTGGCGGTTCCTTTGAGGCCCCTCATGCCCCAGGCTCATTTCATGGTGATTATGCCTATGGCACATATG CCAGCAATTTCAGCGGTGCCCATGGTTTTCCAGAGTACAGCTACCCTGCAGACACCTCCTGGCCTGCTGT GGAGCAAGTTCCATCAAGACCGACCTCTCCTGAGAAGTTTACGGTGCCTCATGTCTGTGCCAGGTTTGGT CCTGGTGGTCAGCTCCTTAAAGTGATTCCCAACCTGCCTTCAGAAGGACAGCCTGCACTGGTGGAGATCC ACAGCTTAGAGACCTTGCTGCAGCACACACCTGAGCAGGAAGAGATGCGCTCCTTCCCAGGACCTCTCGG CAAAGATGATACCCACAAAGTAGATGTTATTAACTTTGCACAGAACAAAGCAACAAAATGTTTGCAGAAT GAAAGTTTAATTGATAAAGAGTCTGCAAGTCTTCTTTGGAAATTCATTATTCTGTTATGCAGACAGAATG GGACTGTTGTGGGAACAGACATTGCAGAGCTTTTGTTACGGGACCATAGAACTGTATGGCTTCCTGGGAA GTCACCCAATGAGGCAAACCTGATAGATTTTACCAATGAGGCTGTGGAACAAGTGGAAGAGGAGGAGTCA GGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAGACAGTGACCACCAGCGTGCTGGAGAAGGAGACTG AGCGGTTCCGTGAGCTCTTGCTGTATGGCCGGAAGAAGGATGCTCTGGAGTCTGCCATGAAAAATGGCTT ATGGGGTCATGCTCTTCTGCTTGCAAGTAAGATGGACAGCCGGACACATGCCCGTGTCATGACCAGGTTT GCCAACAGTCTTCCAATCAATGACCCCCTGCAGACTGTCTACCAGCTCATGTCTGGACGGATGCCTGCTG CATCCACGTGTTGTGGCGATGAGAAGTGGGGAGATTGGAGGCCACATCTTGCAATGGTTTTGTCCAACCT GAACAACAACATGGATGTTGAATCTAGGACCATGGCTACGATGGGTGATACTCTAGCATCAAAAGGCCTG TTGGATGCTGCACACTTCTGCTACCTGATGGCCCAGGTTGGATTTGGGGTTTATACAAAGAAAACTACAA AACTTGTCTTGATAGGCTCAAATCACAGTTTGCCCTTTTTAAAGTTTGCCACCAATGAAGCCATTCAAAG GACGGAGGCTTATGAGTATGCTCAGTCCCTTGGGGCACATACCTGTTCCTTACCTAACTTCCAGGTGTTT AAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGACTTGCCACACAGGCCTTCCACTACTGCGAAGTAA TAGCCAAGAGTGTCCTGACACAGCCTGGTGCATACTCTCCAGTACTGATTAGCCAGTTGACTCAGATGGC TTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGAGAAGCCAGAAGAGGAGTCCTTTGTGGAGCCTGCC TGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATCCAGGAGGGCACTGTGCTGTGGAGCCAGGATGGAA CTGAACCCCAGCAGTGTCGCATTACATCGGGCTCTGAGGTGGAGCAGTCAGATGGCCCTGGACTCAACCA GCAGGCAGGGCCACAGGCCGACAACCCTCTACTGATGCCAAGCACTGAGCCTTTGATGCATGGCGTGCAA CTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGCCAGCCTGCTCACCTTTCCAGGGTGCCGATGTTCC CAGTGCCAATGTCTCGTGGGCCCCTGGAGCTGAGTCCTGCCTATGGACCCCCAGGATCTGCACTTGGCTT CCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCATCCTGGGCAGGCCCTGCCACCCACTACACTAAGT CTCCAGGAAAGTGGGCTTCCACCCCAGGAGGCCAAAAGTCCAGACCCAGAAATGGTGCCACGGGGCTCAC CTGTCAGACACTCCCCTCCGGAGCTCAGTCAAGAGGAGTTTGGCGAGAGCTTCGCTGACCCGGGCTCTTC CAGAACAGCACAGGACTTGGAAACCTCCCCAGTGTGGGATCTTGGCAGCTCCAGTTTGACACGTGCACCA TCTTTGACATCTGATTCTGAAGGGAAGAAACCTGCACAGGCTGTCAAAAAGGAGCCCAAGGAGCCCAAGA AGACTGAGTCCTGGTTCTCTCGTTGGCTGCCTGGAAAAAAAAGGACAGAAGCTTATCTACCAGATGACAA GAACAAATCAATTGTTTGGGATGAAAAGAAAAACCAGTGGGTGAATTTGAATGAACCAGAGGAGGAGAAG AAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGGGTTCCCCAGGTGGCTCCCACTGGGCCTGCAGGAC CACCCACGGCCTCCGTGAATGTGTTTTCTAGAAAAGCAGGTGGGTCCAGAGCTCGCTATGTGGATGTTCT AAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCTTGCTCCTGCAGATTTCTTTGCTCCTCTCGCCCCA CTGCCAATTCCTTCTAACTTATTTGTACCAAACCCAGATGCAGAAGAGCCACAACCTGCAGATGGGACTG GCTGTAGGGGACAGGCACCAGCTGGGACTCAGTCTAAGGCAGAGTCCACCCTGGAACCCAAGGTGGGAAG TTCTACAGTATCAGCCCCTGGACCTGAGCTCTTACCCTCCAAACCAGATGGCTCCCAAGGAGGAGAGGCT CCTGGAGACCATTGTCCTACAGGGGCCCCTCATGGTGGGTCTGTGCCCTTTTACAACCCTGCTCAGTTGG TGCAGGCTTCTGTCACCTCGGGAAATTCAAGGCCAGGGAGGATTGGCCAAAGAAAATATGCGGCATTGAA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR213071 representing NM_153125 Red=Cloning site Green=Tags(s) MQPPPQAVPSGVAGPPPAGNPRSMFWANSPYRKPANNAPVAPITRPLQPVTDPFAFNRQTLQNTPVGSSS KSSLPNLPGPALSVFSQWPGLPVTPTNAGDSSTGLHEPLSGTLSQPRADASLFPPASTPSSLPGLEVSRN AEADPSSGHEVQMLPHSAHYIPGVGPEQPLGGQMNDSGSGPDQPMNRHAPHDGAVTHAASPFLPQPQMPG QWGPAQGGPQPSYQHHSPYLEGPVQNMGLQAASLPHFPPPSSLHQGPGHESHAPQTFTPASLASGEGNEI VHQQSKNHPLSSFPPKHTFEQNSRIGNMWASPELKQNPGVNKEHLLDPAHVNPFTQGNSPENQAHHPPVA ATNHALQEAASGALSMFFQGEETENEENLSSEKAGLDKRLNLDSFSSTSRLGHPPPPGASGVYQAFPRGP SSEAAQEGDAQPYFSQSVGVRLDKQSTVPPANDAWGDVPGTGTRCASGPQCENVENLEFVQNQEVLPRET LSVDPFPLSDQIRYGPLPGPAASRPATVGLTRGGGLNLEAPDTPLHPTRPDSVSSSYSSHSHRSPPGSAR PQELVGTFIQQEVGKLEDDTSGSFFKQIDSSPVGGETDEVTGSQNCCSSLSQPSTPSPPKPTGVFQTSAN SSFEPVKSHLVGVKPVEADRANMVVEVRGTQYCPKKRRAAVAPPDATSGNLEQPPDNMETPCAPQACPLP LSTTGEAGQLVSNTAGTPLDTVRPVPDKRPSARAQGPVKCESPATTLWAQNELPDFGGNVLLAPAAPALY VPVKPKPSEVVHHPEKGMSGQKAWKQGSVPPLQNQDPPGASENLENPPKVGEEEALPVQASSGYASLLSS PPTESLHNQPVLIAQPDQSYNLAQPINFSVSLLNPNEKNQSWGDAVVGERSIVSNNWALGGDPEERAALS GVPASAVTGASLPSSIPQNCAPQGSGSSEMIASQSASWLVQQLSPQTPQSPHPNAEKGPSEFVSSPAGNT SVMLVPPASSTLVPNSNKAKHSSNQEEAVGALDFTLNRTLENPVRMYSPSPSDGPASQQPLPNHPRQSGP GLHNQDHFYQQVTKDAQDQHRLERAQPELVPPRPQNSPQVPQASCPEPSNPESPPTQGQSESLAQPPASP ASVNTGQLLPQPPQASSASVTSTNSSQAAVRSEQLWLHPPPPNTFGPAPQDLASYYYYRPLYDAYQSQYP SPYPSDPGTASLYYQDMYGLYEPRYRPYDSSASAYAENHRYSEPERPSSRASHYSDQLAPRQGYPEGYYN SKSGWSSHSDYYANYYSGQYDYGDPSRWDRYYGSRLRDPRTWDRRYWYDSEHDPYRKDHYAYSDRPEKCD DHWRYDPRFTGSFDDDAEIHRDPYGEEADRRSIHSEHSARSLRSTHSLPSRRSSLSSHSHQSQIYRSHHV TGGSFEAPHAPGSFHGDYAYGTYASNFSGAHGFPEYSYPADTSWPAVEQVPSRPTSPEKFTVPHVCARFG PGGQLLKVIPNLPSEGQPALVEIHSLETLLQHTPEQEEMRSFPGPLGKDDTHKVDVINFAQNKATKCLQN ESLIDKESASLLWKFIILLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEES GEAQLSFLTDSQTVTTSVLEKETERFRELLLYGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRF ANSLPINDPLQTVYQLMSGRMPAASTCCGDEKWGDWRPHLAMVLSNLNNNMDVESRTMATMGDTLASKGL LDAAHFCYLMAQVGFGVYTKKTTKLVLIGSNHSLPFLKFATNEAIQRTEAYEYAQSLGAHTCSLPNFQVF KFIYLCRLAEMGLATQAFHYCEVIAKSVLTQPGAYSPVLISQLTQMASQLRLFDPQLKEKPEEESFVEPA WLVQLQHVERQIQEGTVLWSQDGTEPQQCRITSGSEVEQSDGPGLNQQAGPQADNPLLMPSTEPLMHGVQ LLPTAPQTLPDGQPAHLSRVPMFPVPMSRGPLELSPAYGPPGSALGFPESSRSDPAVLHPGQALPPTTLS LQESGLPPQEAKSPDPEMVPRGSPVRHSPPELSQEEFGESFADPGSSRTAQDLETSPVWDLGSSSLTRAP SLTSDSEGKKPAQAVKKEPKEPKKTESWFSRWLPGKKRTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEK KAPPPPPTSFPRVPQVAPTGPAGPPTASVNVFSRKAGGSRARYVDVLNPSGTQRSEPALAPADFFAPLAP LPIPSNLFVPNPDAEEPQPADGTGCRGQAPAGTQSKAESTLEPKVGSSTVSAPGPELLPSKPDGSQGGEA PGDHCPTGAPHGGSVPFYNPAQLVQASVTSGNSRPGRIGQRKYAALN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_153125
ORF Size	7071 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153125.2, NP_694765.2
RefSeq Size	8754 bp
RefSeq ORF	7074 bp
Locus ID	227648
UniProt ID	E9QAT4
MW	254.7 kDa
Gene Summary	Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:17428803). Mediates the recruitment of MIA3/TANGO to ERES. Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane (By similarity). Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in adipocytes (PubMed:27354378).[UniProtKB/Swiss-Prot Function]

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