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Drosha (NM_026799) Mouse Tagged Lenti ORF Clone
CAT＃: MR211952L4
- LentiORF®
Lenti ORF clone of Drosha (mGFP-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2
"NM_026799" in other vectors (3)
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CNY 13,970.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211952).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026799 |
| ORF Size | 4119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026799.3, NP_081075.3 |
| RefSeq Size | 4580 bp |
| RefSeq ORF | 4122 bp |
| Locus ID | 14000 |
| UniProt ID | Q5HZJ0 |
| Gene Summary | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224350 | Drosha (untagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2, (10ug) |
CNY 17,580.00 |
|
| MR211952 | Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 |
CNY 9,392.00 |
|
| MR211952L3 | Lenti ORF clone of Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 |
CNY 13,970.00 |
