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Tnfaip3 (NM_009397) Mouse Tagged ORF Clone
CAT＃: MR210582
- TrueORF®
Tnfaip3 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1
ORF Plasmid: tGFP
"NM_009397" in other vectors (3)
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CNY 8,512.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A20; Tnfip3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210582 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATATGCGGAAAGCTGTGAAGATACGAGAGA GAACCCCAGAAGACATTTTCAAACCTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACAC GCTGGAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCACAAAGCACTTATTGAC AGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGAAGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGG CTCTGAAAACCAATGGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGTTCAGGA TACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGGAGACAGACACTCGGAACTTTAAATTC CGCTGGCAGCTGGAATCTCTGAAATCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACT GGAATGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAGCCCGAAGTGGACTTCA GTACAATTCCCTGGAAGAAATCCACATATTTGTCCTCAGCAACATCCTCAGAAGACCCATCATTGTCATT TCAGACAAAATGCTAAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGATTTATC TGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATCGTCCTAGGCTATGACAGCCAGCACTT TGTACCCCTGGTGACCCTGAAGGACAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGG GGTAGGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATGAAGGAAAAGCTTCTAA AGGAGTACTTGATAGTGATGGAGATCCCTGTGCAAGGCTGGGACCACGGCACGACTCACCTGATCAACGC TGCAAAATTGGATGAAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTTGTTCAG CACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAGAGCGGCACATGCGCAGAACCCCTTGG AGCCTTCCACACCCCAGCTATCACTCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTC CGTGAACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCAGAGCAAGCTCCCAAAG CTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAGGCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGG AAACCGCTGGAGGACCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGACCACTGC AATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGCAGCATAATGGATTCTGTGAGCGTTGC CACGCCCGGCAGATTAATGCCAGCCACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCA CTCGGACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGCCCAGCTCCAGCCTCAC TTCCAGTATCCCTGCCTCCTGTCACCAACGCTCCAAGTCTGACCCCTCACAACTCATCCAAAGTCTCACT CCACACTCTTGCCACCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGACTCCAG GAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATGTATTTTGGGACTCCAGAAAACAAGGG CTTTTGCACTCTATGTTTCATCGAATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGT TCCCCGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGCACACTCGGAAGCACCA TGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGAAGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAAC GGAAGAACAGCTGAGATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGGCTGAAA TGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGAGGAACTCTGTATGGAGTGCCAGCACC TAAGCCAACGAGTAGGTTCTGTGGCCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTG CCGGGCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAATGAGTGCTACCAGTTC AAGCAGATGTATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210582 protein sequence
Red=Cloning site Green=Tags(s) MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMHRYTLEMFRTCQFCPQFREIIHKALID RSVQASLESQKKLNWCREVRKLVALKTNGDGNCLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKF RWQLESLKSQEFVETGLCYDTRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVI SDKMLRSLESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLVNRDR GRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEANLPKEINLVDDYFELVQ HEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNCPFFMSVNTQPLFHECSERRQKNQSKLPK LNSKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERC HARQINASHTADPGKCQACLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLT PHSCHRTGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASEKAG SPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRTTQVASRLK CARASCKNILACRSEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECYQF KQMYG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009397 |
| ORF Size | 2325 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009397.1, NM_009397.2, NM_009397.3, NP_033423.3 |
| RefSeq Size | 4437 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 21929 |
| UniProt ID | Q60769 |
| MW | 87.7 kDa |
| Gene Summary | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3 / A20
,null,
Journal of Cellular and Molecular Medicine
,PubMed ID 35841281
[Tnfaip3]
|
|
Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages
,Liu, SS;Lv, XX;Liu, C;Qi, J;Li, YX;Wei, XP;Li, K;Hua, F;Cui, B;Zhang, XW;Yu, JJ;Yu, JM;Wang, F;Shang, S;Zhao, CX;Hou, XY;Yao, ZG;Li, PP;Li, X;Huang, B;Hu, ZW;,
Immunity
,PubMed ID 31471107
[TNFAIP3]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210582 | Tnfaip3 (tGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3) |
CNY 8,460.00 |
|
| MR210582L3 | Lenti ORF clone of Tnfaip3 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1 |
CNY 9,600.00 |
|
| MR210582L4 | Lenti ORF clone of Tnfaip3 (mGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1 |
CNY 10,912.00 |
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