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Nbn (NM_013752) Mouse Tagged ORF Clone
CAT＃: MR210466
- TrueORF®
Nbn (Myc-DDK-tagged) - Mouse nibrin (Nbn)
ORF Plasmid: tGFP
"NM_013752" in other vectors (4)
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CNY 5,504.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nbs1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210466 representing NM_013752
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAGCTGCTCCCGGCCGCCGGTGCGGCTCCAGGAGAACCATACCGACTTTTGGCCGGCGTGGAGT ACGTTGTTGGGAGGAAAAACTGTGGCATTCTGATTGAAAATGATCAGTCAATCAGTCGAAACCATGCTGT CTTAACAGTAAACTTCCCTGTAACCAGTTTGAGTCAAACAGATGAAATTCCTACATTAACAATAAAAGAT AATTCTAAGTATGGAACCTTTGTTAATGAAGAAAAAATGCAGACTGGTCTTTCCTGCACGTTGAAGACAG GAGATAGAGTTACCTTTGGGGTGTTTGAAAGTAAATTCAGAGTAGAATACGAGCCCTTGGTTGTTTGTTC TTCTTGTTTAGATGTCTCTGGGAAAACTGTTTTAAATCAAGCTATTTTACAGCTTGGAGGACTTACCGCA AACAACTGGACGGAAGAATGTACCCACCTTGTCATGTCGGCAGTTAAAGTCACCATTAAAACTATATGTG CACTCATTTGTGGACGTCCAATTATAAAACCAGAATACTTTTCTGAATTTCTCAAAGCAGTTGAATCTAA GAAACAGCCTCCAGATATTGAAAGTTTTTACCCACCCATTGATGAACCAGCTATTGGAAGTAAAAGTGTT GATCTGTCAGGGCGACATGAAAGAAAACAGATCTTCAAAGGAAAAACATTTGTGTTTTTAAATGCCAAGC AGCACAAGAAACTCAGCTCGGCAGTTGCTTTCGGAGGTGGAGAAGCCAGGCTGATGGCAGAAGACGACGA AGAGGAACAGAGCTTCTTTTCAGCTCCCGGAACCTGCGTTGTTGATGTAGGAATAACGAATACACAGCTC ATAATTTCACACTCCCAGAAAAAATGGATTCATTTGATAATGGATACACTTCAAAGGAATGGTCTCAGAC CTATTCCTGAAGCGGAGATTGGATTGGCTGTTATTTTTATGACTACAGAGAATTACTGTAATCCGCAGGG CCAGCCTTGTACAGAATTAAAGACAACGACTCCAGGACCAAGCCTTTCCCAAGTCCTGTCAGCCAATGGA AAAATAATCCCAAGTGCTCCAGTGAATATGACCACATACGTAGCTGACACAGAATCAGAGCCAGCAGATA CATGTATGCCTTTGAGTGAAAGACCAGAAGAAGTAAAGATCCCTGGACTGGAACAAAGCTCTAGGAAACT TTCACAAGAAACATTCAATATAAAGGAGGCCCCTAAACCAAGCTCCAAAGCTAACAACGTAGCATCAGAT ACGCTGGTTAGAGGAAAGACCCCGAGCTATCAGCTTTCTCCAATGAAATTTCCTGTTGCAAATAAAAATA AGGATTGGACTTCTCAGCAGCAGCAGAACTCCATCAAAAACTACTTCCAGCCATGCACCAGAAAAAGGGA AAGGGATGAAGACAACCCAGAGCTGTCCTCGTGCAAATCATCCAGGATGGAGCTGTCCTGTTCTCTTTTA GAACAAACACAACCTGCCGGACCCTCACTGTGGAAAAGCAAGGAGCATCAGTCTCAGAATGCGACCCTGG ACAGGGAAGCCGACACCTCATCTGTGGGTGGGATGGACATAGAACTCAACAGGAAGAGTCCTGACAGGAA ACCACTTCCCACAGAAACTCTTAGACCCAGAAAAAGAAAAGATGTTGATTTAGCTACAGAAGAGGAAGTC TTGGAAGAGTTACTGAGGAGTACAAAGCCAGAGTTGGCAGTTCAAGTGAAGGTTGAAAAGCAGGAGGCAG ATGACACCATCAGAAAAAAGCCAAGGATGGACGCAGAGAGGAATCGGCCCCTGAATGGTGGATCAGAGCC GGAAAGCAACAGCGCTCTTCAAGAAGATGAAAGAGAAAAGAAAGATGAACTTCAGACAGAGTCGTGGTCA ACAAAACATGAAATAGCTAATAGTGATGGTCTTCAGGACAGCAGTGAGGAGCTGCCACGGAAACTGCTGC TGACTGAATTTAGGTCATTGGTTGTCAGTAACCACAACTCCACCTCCAGAAATCTATGTGTAAATGAATG TGGTCCACTGAAGAATTTCAAGAAATTCAAGAAGGCGACATTTCCTGGGGCAGGAAAGCTTCCACACATC ATCGGAGGATCAGACTTAGTAGGCCACCATGCTCGAAAGAATACCGAGTTAGAAGAATGGTTGAAACAGG AAATGGAGGTACAGAAACAACAAGCAAAGGAGGAATCTCTTGCTGATGATCTGTTTAGATATAATCCTAA TGTAAAAAGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210466 representing NM_013752
Red=Cloning site Green=Tags(s) MWKLLPAAGAAPGEPYRLLAGVEYVVGRKNCGILIENDQSISRNHAVLTVNFPVTSLSQTDEIPTLTIKD NSKYGTFVNEEKMQTGLSCTLKTGDRVTFGVFESKFRVEYEPLVVCSSCLDVSGKTVLNQAILQLGGLTA NNWTEECTHLVMSAVKVTIKTICALICGRPIIKPEYFSEFLKAVESKKQPPDIESFYPPIDEPAIGSKSV DLSGRHERKQIFKGKTFVFLNAKQHKKLSSAVAFGGGEARLMAEDDEEEQSFFSAPGTCVVDVGITNTQL IISHSQKKWIHLIMDTLQRNGLRPIPEAEIGLAVIFMTTENYCNPQGQPCTELKTTTPGPSLSQVLSANG KIIPSAPVNMTTYVADTESEPADTCMPLSERPEEVKIPGLEQSSRKLSQETFNIKEAPKPSSKANNVASD TLVRGKTPSYQLSPMKFPVANKNKDWTSQQQQNSIKNYFQPCTRKRERDEDNPELSSCKSSRMELSCSLL EQTQPAGPSLWKSKEHQSQNATLDREADTSSVGGMDIELNRKSPDRKPLPTETLRPRKRKDVDLATEEEV LEELLRSTKPELAVQVKVEKQEADDTIRKKPRMDAERNRPLNGGSEPESNSALQEDEREKKDELQTESWS TKHEIANSDGLQDSSEELPRKLLLTEFRSLVVSNHNSTSRNLCVNECGPLKNFKKFKKATFPGAGKLPHI IGGSDLVGHHARKNTELEEWLKQEMEVQKQQAKEESLADDLFRYNPNVKRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013752 |
| ORF Size | 2253 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013752.3, NP_038780.3 |
| RefSeq Size | 2491 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 27354 |
| UniProt ID | Q9R207 |
| MW | 84.2 kDa |
| Gene Summary | Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202265 | Nbn (untagged) - Mouse nibrin (Nbn), (10ug) |
CNY 2,000.00 |
|
| MG210466 | Nbn (tGFP-tagged) - Mouse nibrin (Nbn) |
CNY 6,370.00 |
|
| MR210466L3 | Lenti ORF clone of Nbn (Myc-DDK-tagged) - Mouse nibrin (Nbn) |
CNY 7,700.00 |
|
| MR210466L4 | Lenti ORF clone of Nbn (mGFP-tagged) - Mouse nibrin (Nbn) |
CNY 7,700.00 |
