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Smpd3 (NM_021491) Mouse Tagged ORF Clone
CAT＃: MR209795
- TrueORF®
Smpd3 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3)
ORF Plasmid: tGFP
"NM_021491" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4631433G07Rik; AI427456; AW537966; fro; nSMase2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209795 representing NM_021491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTGTACACGACCCCCTTTCCTAATAGCTGTCTGTCTGCCCTCCACGCCGTGTCCTGGGCCCTCA TCTTCCCATGTTACTGGCTGGTGGACCGGCTGTTGGCCTCTTTCATACCCACCACCTATGAGAAGCGCCA GAGGGCAGATGACCCCTGCTGCCTGCAGCTGTTCTGCACGGTCCTCTTCACGCCAGTCTACCTGGCTCTG CTTGTGGCTGCACTGCCCTTTGCCTTTCTCGGGTTCATCTTCTGGTCTCCACTGCAGTCTGCCCGCCGGC CCTACTCCTACTCCCGGCTAGAAGACAAGAACCCAGCTGGTGGGGCAGCCCTGCTTAGTGAATGGAAGGG CACGGGGGCAGGCAAAAGCTTTTGCTTCGCCACGGCCAATGTCTGCCTCCTCCCCGACTCACTCGCGAGG CTCAATAATGTGTTCAACACTCAAGCTCGGGCCAAAGAGATTGGGCAGAGAATCCGCAATGGGGCCGCCA GGCCTCAGATCAAGATCTACATCGATTCTCCCACCAACACCTCCATCAGTGCAGCCAGCTTCAGCAGCCT GGTGTCGCCACAGGGTGGTGATGGATCCAGAGCTGTTCCTGGGAGCATTAAGAGGACAGCCTCTGTGGAA TACAAGGGGGATGGTGGGCGTCACCCCAGCGACGAGGCTGCCAACGGCCCAGCCTCTGGAGAACAGGCTG ACGGAAGCCTTGAGGACAGCTGCATTGTGCGAATTGGTGGCGAGGAAGGAGGTCGGCCACAAGAAGCTGA TGACCCTGCTGCCGGGAGCCAAGCCAGGAATGGGGCTGGTGGGACCCCAAAGGGCCAGACACCCAACCAC AATCAGCGAGATGGGGATTCTGGGAGCCTGGGCAGCCCCTCAGCCTCCAGGGAGTCCTTGGTGAAGGCAC GGGCTGGGCAAGACAGTGGTGGCAGCGGAGAGCCAGGGGCTAACAGCAAGCTGCTGTATAAGACCTCTGT GGTGAAGAAGGCAGCAGCCCGCAGGAGGCGCCACCCTGATGAAGCCTTTGACCACGAGGTCTCAGCCTTC TTCCCGGCCAATCTGGACTTCCTGTGCCTGCAGGAGGTGTTTGACAAGCGTGCGGCTGCTAAGTTGAAAG AGCAGCTACACGGCTACTTCGAGTACATCCTGTATGATGTTGGGGTCTACGGTTGTCATGGTTGCTGCAA TTTCAAATGTCTCAACAGCGGTCTCTTCTTCGCCAGCCGCTACCCTGTCATGGACGTGGCCTATCACTGT TACCCCAATGGGTGCAGCTTCGATGCCCTGGCCTCTAAGGGAGCTCTGTTTCTCAAGGTGCAGGTGGGAA GCACACCTCAGGACCAAAGAATTGTTGGGTACATCGCCTGCACACACCTGCATGCCCCACCAGAGGACAG TGCCGTCCGGTGTGAGCAGCTGGACCTGCTTCAGGACTGGCTGGCTGATTTCCGAAAATCTACCTCCTCG ACCAGCACAGCCAACCCCGAGGAGCTGGTGGTGTTTGACGTCATCTGTGGAGATTTGAACTTTGATAACT GCTCTTCTGATGACAAACTGGAGCAGCAGCACTCGCTCTTTACTCGCTACAAAGACCCCTGCCGCCTTGG GCCCGGGGAGGAGAAGCCTTGGGCCATTGGCACCCTGCTGGACACAAACGGTCTCTATGATGAGGATGTG TGTACCCCGGACAACCTTCAAAAGGTGTTGGAGAGCGAGGAAGGCCGCAGGGAGTATCTGGCCTTCCCCA CCAGCAAGAGCCCAGGTGCTGGGCAGAAGGGCCGGAAGGATCTGCTGAAGGGCAATGGCCGCCGCATCGA CTACATGCTGCACGCCGAGGAGGGGCTGTGCCCTGACTGGAAGGCTGAGGTAGAAGAATTCAGTTTTATC ACCCAGCTGTCCGGCCTGACCGACCACCTCCCCGTGGCCATGCGGCTGATGGTGTCTGCAGGGGAAGAGG AGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209795 representing NM_021491
Red=Cloning site Green=Tags(s) MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLLASFIPTTYEKRQRADDPCCLQLFCTVLFTPVYLAL LVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKNPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLAR LNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGSRAVPGSIKRTASVE YKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRPQEADDPAAGSQARNGAGGTPKGQTPNH NQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGANSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAF FPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHC YPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAVRCEQLDLLQDWLADFRKSTSS TSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDTNGLYDEDV CTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFI TQLSGLTDHLPVAMRLMVSAGEEEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021491 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021491.4 |
| RefSeq Size | 5148 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 58994 |
| UniProt ID | Q9JJY3 |
| MW | 71.6 kDa |
| Gene Summary | Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Acts as a regulator of postnatal development and participates in bone and dentin mineralization. Overexpression enhances cell death, suggesting that it may be involved in apoptosis control.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202307 | Smpd3 (untagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3), (10ug) |
CNY 2,000.00 |
|
| MG209795 | Smpd3 (tGFP-tagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3) |
CNY 5,040.00 |
|
| MR209795L3 | Lenti ORF clone of Smpd3 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3) |
CNY 7,280.00 |
|
| MR209795L4 | Lenti ORF clone of Smpd3 (mGFP-tagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3) |
CNY 6,460.00 |
