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Pias1 (NM_019663) Mouse Tagged ORF Clone
CAT＃: MR209770
- TrueORF®
Pias1 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 1 (Pias1)
ORF Plasmid: tGFP
"NM_019663" in other vectors (4)
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CNY 4,848.00
CNY 5,700.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2900068C24Rik; Ddxbp1; GBP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209770 representing NM_019663
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAAGTACTGTTGG GCTACGCTGGGAGGAACAAGCACGGACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGC TGGCTGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCAGAAAATTATG ACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCA TTCCACAGCTCACTTATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAA ACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACCCGGACATAAAGCTGCAGAAG CTACCATTCTATGACCTGTTGGATGAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCT TTCGGGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCTCCATGGATAT TTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCA CAAGAAGATCACTTCCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC TTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGACCAATTAATATCACCTCACTTGTCCG ATTGTCCACGACAGTACCAAATACCATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATG GCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAGGGAATAAGGA ATCCGGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAACTGCAGATCCAGATAGTGAGATAGCTACTAC CAGCCTACGGGTTTCGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACC TGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGAGAAAAAACCAACATGGGTTT GTCCTGTCTGTGATAAGAAGGCCCCATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAA GTACTGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAGGTCAAAGAAG GAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGG TAGCGTCCCACAACCAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGACAGCTC GTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCACCACTA AGTAATAAAGGCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTG TAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGACGCCTATGCC TTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCCTG CTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCCGTATA CCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCTCTGCCAGCCACCAACGGAAG CAGTAGCGGCAGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCC AGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209770 representing NM_019663
Red=Cloning site Green=Tags(s) MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIM TPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQK LPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCP QEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSM AVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALT CSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKK EVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVIDLTIDSSSDEEEEEPPAKRTCPSLSPTSPL SNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSL LAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSNSLRESHGHGVA SRSSADTASIFGIIPDIISLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019663 |
| ORF Size | 1953 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019663.3 |
| RefSeq Size | 3856 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 56469 |
| UniProt ID | O88907 |
| MW | 72.1 kDa |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA (By similarity). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (PubMed:24061474).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Protein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPβ in 3T3L1 cells
,Watanabe, Y;Watanabe, K;Fujioka, D;Nakamura, K;Nakamura, T;Uematsu, M;Bachschmid, MM;Matsui, R;Kugiyama, K;,
FASEB J.
,PubMed ID 32141127
[PIAS1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202291 | Pias1 (untagged) - Mouse protein inhibitor of activated STAT 1 (Pias1), (10ug) |
CNY 2,000.00 |
|
| MG209770 | Pias1 (tGFP-tagged) - Mouse protein inhibitor of activated STAT 1 (Pias1) |
CNY 6,270.00 |
|
| MR209770L3 | Lenti ORF clone of Pias1 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 1 (Pias1) |
CNY 7,600.00 |
|
| MR209770L4 | Lenti ORF clone of Pias1 (mGFP-tagged) - Mouse protein inhibitor of activated STAT 1 (Pias1) |
CNY 7,600.00 |
