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Ephx2 (NM_007940) Mouse Tagged ORF Clone
CAT＃: MR208783
- TrueORF®
Ephx2 (Myc-DDK-tagged) - Mouse epoxide hydrolase 2, cytoplasmic (Ephx2)
ORF Plasmid: tGFP
"NM_007940" in other vectors (4)
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CNY 4,136.00
CNY 4,940.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CEH; Eph2; SEH; sEP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208783 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGTGTAGCCGCGTTCGACCTTGACGGAGTGCTGGCCCTCCCCTCTATCGCCGGGGCTTTCC GCCGCAGCGAAGAGGCCCTGGCACTGCCTAGAGACTTCCTGCTTGGTGCGTACCAGACGGAATTCCCAGA GGGACCCACTGAGCAACTCATGAAAGGGAAGATCACATTTTCGCAGTGGGTACCACTCATGGATGAAAGC TACAGGAAGTCCTCCAAAGCCTGTGGAGCCAATCTACCTGAGAATTTCTCCATAAGTCAAATATTCAGCC AAGCTATGGCAGCAAGAAGCATCAACCGCCCCATGCTTCAGGCAGCCATTGCTCTCAAAAAGAAAGGATT CACAACATGCATTGTCACCAACAACTGGCTGGACGACGGAGACAAGAGAGACAGCCTGGCCCAGATGATG TGTGAGCTGAGCCAACACTTTGACTTCCTGATAGAGTCCTGTCAGGTTGGGATGATCAAGCCTGAGCCTC AGATCTACAATTTTTTACTGGATACCCTGAAGGCAAAACCCAATGAGGTTGTTTTCCTAGATGACTTTGG AAGTAATCTGAAGCCAGCCCGTGACATGGGGATGGTTACCATCCTGGTCCACAACACAGCCTCCGCTCTG AGAGAACTGGAGAAGGTCACAGGGACACAGTTTCCTGAGGCCCCACTGCCAGTCCCATGCAATCCAAATG ACGTCAGCCATGGATATGTGACAGTGAAGCCAGGGATCCGCCTGCATTTTGTGGAGATGGGCTCTGGCCC TGCCCTATGCCTTTGCCATGGGTTTCCTGAGAGCTGGTTTTCTTGGAGGTACCAGATCCCTGCTCTGGCC CAGGCAGGCTTTCGTGTTCTGGCTATAGACATGAAAGGCTATGGAGACTCATCTTCTCCTCCTGAAATAG AAGAATATGCCATGGAATTGCTGTGTAAGGAGATGGTGACATTCCTGGATAAGCTGGGAATCCCTCAAGC AGTGTTCATTGGCCATGACTGGGCTGGTGTGATGGTGTGGAACATGGCTCTCTTCTACCCTGAGAGAGTG AGGGCTGTGGCCAGTTTGAACACGCCGTTTATGCCACCAGATCCTGATGTGTCTCCCATGAAAGTTATCC GATCTATCCCAGTTTTCAATTATCAGCTGTACTTTCAAGAACCAGGAGTGGCCGAGGCTGAACTGGAGAA GAACATGAGTCGGACTTTCAAAAGCTTCTTCCGAGCCAGTGATGAGACAGGTTTCATCGCTGTGCATAAA GCCACTGAAATAGGGGGAATCCTTGTGAATACTCCAGAAGATCCCAACCTCAGCAAAATTACTACTGAGG AAGAAATAGAGTTTTACATACAGCAGTTCAAGAAGACTGGCTTCAGAGGTCCTCTGAACTGGTACCGGAA CACAGAAAGAAACTGGAAGTGGAGCTGTAAAGGGTTGGGACGAAAGATCTTGGTCCCAGCCCTGATGGTC ACAGCTGAGAAGGACATTGTACTCCGTCCTGAAATGTCCAAGAACATGGAAAAGTGGATCCCTTTCCTGA AAAGGGGACACATTGAAGACTGTGGTCACTGGACACAGATAGAGAAACCAACTGAGGTGAACCAGATTCT CATCAAGTGGCTGCAGACTGAAGTCCAGAACCCATCAGTGACCTCCAAGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208783 protein sequence
Red=Cloning site Green=Tags(s) MALRVAAFDLDGVLALPSIAGAFRRSEEALALPRDFLLGAYQTEFPEGPTEQLMKGKITFSQWVPLMDES YRKSSKACGANLPENFSISQIFSQAMAARSINRPMLQAAIALKKKGFTTCIVTNNWLDDGDKRDSLAQMM CELSQHFDFLIESCQVGMIKPEPQIYNFLLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVHNTASAL RELEKVTGTQFPEAPLPVPCNPNDVSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALA QAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERV RAVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRASDETGFIAVHK ATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKILVPALMV TAEKDIVLRPEMSKNMEKWIPFLKRGHIEDCGHWTQIEKPTEVNQILIKWLQTEVQNPSVTSKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007940 |
| ORF Size | 1665 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007940.4 |
| RefSeq Size | 2062 bp |
| RefSeq ORF | 1665 bp |
| Locus ID | 13850 |
| UniProt ID | P34914 |
| MW | 62.5 kDa |
| Gene Summary | Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Soluble epoxide hydrolase modulates immune responses in activated astrocytes involving regulation of STAT3 activity
,Hung, CC;Lee, YH;Kuo, YM;Hsu, PC;Tsay, HJ;Hsu, YT;Lee, CC;Liang, JJ;Shie, FS;,
J Neuroinflammation
,PubMed ID 31176371
[EPHX2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200854 | Ephx2 (untagged) - Mouse epoxide hydrolase 2, cytoplasmic (Ephx2), (10ug) |
CNY 2,000.00 |
|
| MG208783 | Ephx2 (tGFP-tagged) - Mouse epoxide hydrolase 2, cytoplasmic (Ephx2) |
CNY 5,420.00 |
|
| MR208783L3 | Lenti ORF clone of Ephx2 (Myc-DDK-tagged) - Mouse epoxide hydrolase 2, cytoplasmic (Ephx2) |
CNY 6,840.00 |
|
| MR208783L4 | Lenti ORF clone of Ephx2 (mGFP-tagged) - Mouse epoxide hydrolase 2, cytoplasmic (Ephx2) |
CNY 6,840.00 |
