Fads1 (NM_146094) Mouse Tagged ORF Clone
CAT#: MR207139
- TrueORF®
Fads1 (Myc-DDK-tagged) - Mouse fatty acid desaturase 1 (Fads1)
ORF Plasmid: tGFP
"NM_146094" in other vectors (4)
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CNY 3,330.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 0710001O03Rik; A930006B21Rik; AI317215; DSD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207139 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGACCCGGTGCCGACCCCTGGCCCGGCCTCCGCCCAGCTCCGCCAAACGCGCTACTTTACTT GGGAGGAGGTGGCGCAGCGCTCCGGGCGGGAGAAGGAGCGATGGCTCGTGATCGACCGGAAGGTGTACAA CATCAGCGACTTCAGCCGCCGCCACCCGGGGGGCTCCCGGGTCATCAGCCACTACGCGGGTCAGGATGCC ACGGATCCTTTTGTGGCATTCCACATCAACAAGGGTCTTGTGAGAAAGTATATGAACTCTCTTCTGATTG GAGAGCTGGCTCCGGAGCAACCCAGCTTTGAACCCACCAAGAATAAAGCGCTAACTGATGAATTCCGGGA GCTGCGGGCCACAGTAGAGCGAATGGGCCTCATGAAGGCCAACCACCTCTTCTTCCTGGTCTACCTGCTT CACATCCTGCTGCTGGATGTGGCTGCCTGGCTCACCCTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCA TCCTCTGTGCAGTACTGCTCAGTACAGTTCAGGCTCAGGCAGGTTGGCTACAGCATGACTTTGGGCACCT GTCAGTCTTTGGCACCTCGACATGGAATCACCTGCTACATCATTTTGTGATTGGCCACCTGAAGGGGGCC CCCGCCAGCTGGTGGAACCACATGCATTTCCAGCACCATGCCAAGCCTAACTGCTTCCGCAAGGACCCCG ATATCAACATGCACCCCCTCTTCTTCGCCCTGGGGAAGGTCCTTCCTGTGGAGCTCGGGAGGGAAAAGAA GAAGCACATGCCATACAACCATCAGCACAAGTACTTCTTCCTCATCGGACCCCCAGCCTTGCTGCCTCTA TACTTCCAGTGGTATATTTTCTATTTTGTGGTTCAGCGGAAGAAATGGCTGGACTTGGCCTGGATGCTCA GCTTCTATGCCCGCATCTTCTTCACTTACATGCCGCTGCTGGGGCTGAAAGGCTTCCTGGGCCTTTTCTT CATTGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGATGAACCATATCCCCATGCACATT GATCACGACCGGAATGTGGACTGGGTCTCCACCCAGCTGCAGGCAACCTGCAACATTCACCAATCAGCCT TCAACAACTGGTTCAGTGGCCACCTAAATTTCCAGATTGAACACCACCTCTTCCCCACCATGCCGCGGCA CAACTACCACAAGGTGGCACCCCTAGTACAATCCCTGTGCGCCAAGTACGGCATCAAGTATGAGTCCAAG CCCCTGCTCACAGCCTTCGCGGACATTGTTTACTCCCTGAAGGAGTCAGGGCAACTCTGGTTGGACGCTT ACCTTCACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207139 protein sequence
Red=Cloning site Green=Tags(s) MAPDPVPTPGPASAQLRQTRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDA TDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLVYLL HILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGA PASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPL YFQWYIFYFVVQRKKWLDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHI DHDRNVDWVSTQLQATCNIHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESK PLLTAFADIVYSLKESGQLWLDAYLHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_146094 |
ORF Size | 1344 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146094.1, NM_146094.2, NP_666206.1 |
RefSeq Size | 3474 bp |
RefSeq ORF | 1344 bp |
Locus ID | 76267 |
UniProt ID | Q920L1 |
MW | 52.4 kDa |
Gene Summary | Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (Probable). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (PubMed:22534642). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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FADS3 is a delta14Z sphingoid base desaturase that contributes to gender differences to the human plasma sphingolipidome
,Karsai, G;Lone, MA;Kutalik, Z;Brenna, JT;Li, H;Pan, D;von Eckardstein, A;Hornemann, T;,
J. Biol. Chem.
,PubMed ID 31862735
[FADS1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204525 | Fads1 (untagged) - Mouse fatty acid desaturase 1 (Fads1), (10ug) |
CNY 2,000.00 |
|
MG207139 | Fads1 (tGFP-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 3,710.00 |
|
MR207139L3 | Lenti ORF clone of Fads1 (Myc-DDK-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 5,230.00 |
|
MR207139L4 | Lenti ORF clone of Fads1 (mGFP-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 5,230.00 |