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Wipi2 (NM_178398) Mouse Tagged ORF Clone
CAT＃: MR207108
- TrueORF®
Wipi2 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2)
ORF Plasmid: tGFP
"NM_178398" in other vectors (4)
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CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110018O08Rik; 2510001I10Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207108 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTGGCGAGCCAGAGCGGAGAGGCCGGCGCCGGCCAGCTGCTGTTCGCCAACTTCAACCAGGATA ACACGTCCCTAGCTGTTGGTAGTAAGTCCGGGTATAAGTTTTTCTCCCTTTCTTCTGTGGATAAGCTGGA ACAGATCTATGAATGCACTGACACTGAAGATGTCTGCATTGTGGAGAGATTGTTCTCAAGCAGCTTGGTG GCCATTGTCAGCCTCAAAGCTCCCAGGAAGCTGAAGGTTTGCCATTTTAAGAAGGGAACTGAGATATGCA ACTACAGCTACTCCAACACTATCCTGGCTGTGAAGCTGAACAGGCAGCGGCTCATTGTGTGTCTGGAAGA GTCGCTCTACATACACAACATCCGGGACATGAAGGTACTTCATACCATCCGAGAGACACCCCCAAACCCT GCAGGCTTGTGTGCACTGTCAATAAACAATGACAACTGCTACTTGGCGTATCCAGGGAGTGCGAGCATTG GAGAGGTGCAGGTCTTCGACACCATTAACTTGAGAGCTGCAAACATGATCCCAGCTCATGATAGTCCCTT AGCAGCCCTGGCTTTTGATGCAAGTGGGACCAAGCTTGCCACTGCTTCTGAGAAGGGGACTGTGATTCGG GTGTTTTCCATTCCAGAGGGACAGAAGCTGTTTGAGTTCAGGAGAGGAGTGAAGAGGTGTGTGAGCATCT GCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCATCCAGCAACACCGAAACAGTGCACATCTT CAAACTTGAGGCTGTGAGGGAAAAACCTCCGGAAGAGCCCACCACCTGGACTGGCTACTTTGGGAAGGTT CTCATGGCATCTACCAGCTACCTGCCCTCACAAGTGACAGAAATGTTCAACCAGGGCAGGGCCTTCGCCA CTGTCCGCCTGCCATTCTGTGGCCACAAAAACATCTGCTCACTAACCACAATTCAGAAGATCCCACGGTT GCTGGTAGGAGCATCAGATGGCTATTTGTACATGTACAACCTGGACCCCCAGGAGGGAGGCGAGTGTGCG CTGATGCGTCAGCACAGGCTTGATGGCAGTATGGAGACGACCAGTGAAATCGTAGACTCTGCATCTCATG ACTGCCCCTTAGCAACACAGACGTACGGCACAGCAGCTGCCAAAGGTGCCTATGTGCCCTCGTCCCCCAC AAGACTTGGGAAAGGGCAGGACGCCAACTTAGAAGCCTACACAGATGACCTGGGTGCTGTGGGTGGTGCA TGCCTAGAGGATGAAGCCAGCGCTCTGCGCCTGGATGAAGACAGCGAACATCCTCCCATGATTCTCCGGA CTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207108 protein sequence
Red=Cloning site Green=Tags(s) MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLV AIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNP AGLCALSINNDNCYLAYPGSASIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIR VFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLEAVREKPPEEPTTWTGYFGKV LMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLTTIQKIPRLLVGASDGYLYMYNLDPQEGGECA LMRQHRLDGSMETTSEIVDSASHDCPLATQTYGTAAAKGAYVPSSPTRLGKGQDANLEAYTDDLGAVGGA CLEDEASALRLDEDSEHPPMILRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178398 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178398.4, NP_848485.1 |
| RefSeq Size | 3913 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 74781 |
| UniProt ID | Q80W47 |
| MW | 48.5 kDa |
| Gene Summary | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215915 | Wipi2 (untagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2), (10ug) |
CNY 5,800.00 |
|
| MG207108 | Wipi2 (tGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2) |
CNY 4,940.00 |
|
| MR207108L3 | Lenti ORF clone of Wipi2 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2) |
CNY 6,370.00 |
|
| MR207108L4 | Lenti ORF clone of Wipi2 (mGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2) |
CNY 6,370.00 |
