Adrb2 (NM_007420) Mouse Tagged ORF Clone

CAT#: MR206632

  • TrueORF®

Adrb2 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 2 (Adrb2)

ORF Plasmid: DDK tGFP



  "NM_007420" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,330.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-beta2-Adrenoceptor (extracellular)
    • 50 ul

CNY 11,000.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Adrb; Adrb-2; Bad; Badm; Gpc; Gpcr7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206632 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCACACGGGAACGACAGCGACTTCTTGCTGGCACCCAACGGAAGCCGAGCGCCAGACCACGACG
TCACTCAGGAACGGGACGAAGCGTGGGTTGTGGGCATGGCCATCCTCATGTCGGTTATCGTCCTGGCCAT
CGTGTTTGGCAACGTGCTGGTCATCACAGCCATTGCCAAGTTCGAGCGACTACAAACCGTCACCAACTAC
TTCATAATCTCCTTGGCGTGTGCTGATCTAGTCATGGGCCTAGCGGTGGTGCCGTTTGGGGCCAGTCACA
TCCTTATGAAAATGTGGAATTTTGGCAACTTCTGGTGCGAGTTCTGGACTTCCATTGATGTGTTGTGCGT
CACAGCCAGCATCGAGACCCTGTGCGTGATTGCAGTGGATCGCTATGTTGCTATCACATCGCCCTTCAAG
TACCAGAGCCTGCTGACCAAGAATAAGGCCCGAGTGGTCATCCTGATGGTATGGATTGTATCTGGCCTTA
CCTCCTTTTTGCCTATCCAGATGCACTGGTACCGTGCCACCCACAAGAAAGCTATCGATTGTTACACCGA
GGAGACTTGCTGTGACTTCTTCACGAACCAGGCCTACGCCATCGCGTCCTCGATTGTGTCTTTCTACGTG
CCCCTGGTGGTGATGGTCTTTGTCTATTCCCGGGTCTTCCAGGTGGCCAAAAGGCAGCTGCAGAAGATAG
ACAAATCTGAAGGAAGATTCCACGCCCAAAACCTCAGCCAGGTGGAGCAGGATGGGCGGAGCGGCCACGG
ACTCCGAAGGTCCTCCAAGTTCTGCTTGAAAGAGCACAAAGCCCTCAAGACTTTAGGCATCATCATGGGC
ACATTCACCCTCTGCTGGCTGCCCTTCTTCATTGTCAATATCGTGCACGTTATCAGGGACAACCTCATCC
CTAAGGAAGTTTACATCCTCCTTAACTGGTTGGGCTACGTCAACTCTGCCTTCAATCCTCTTATCTACTG
TCGGAGTCCAGATTTCAGGATTGCCTTTCAAGAGCTTCTGTGCCTTCGCAGGTCTTCTTCGAAAACCTAT
GGGAACGGCTACTCTAGCAATAGCAACGGCAGAACGGACTACACAGGGGAGCCAAACACTTGTCAGCTGG
GGCAGGAGAGAGAACAGGAACTGCTGTGTGAGGATCCCCCAGGCATGGAAGGCTTTGTGAACTGTCAAGG
TACTGTGCCTAGCCTTAGCGTTGACTCCCAAGGAAGGAACTGTAGTACAAATGACTCGCCACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206632 protein sequence
Red=Cloning site Green=Tags(s)

MGPHGNDSDFLLAPNGSRAPDHDVTQERDEAWVVGMAILMSVIVLAIVFGNVLVITAIAKFERLQTVTNY
FIISLACADLVMGLAVVPFGASHILMKMWNFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYVAITSPFK
YQSLLTKNKARVVILMVWIVSGLTSFLPIQMHWYRATHKKAIDCYTEETCCDFFTNQAYAIASSIVSFYV
PLVVMVFVYSRVFQVAKRQLQKIDKSEGRFHAQNLSQVEQDGRSGHGLRRSSKFCLKEHKALKTLGIIMG
TFTLCWLPFFIVNIVHVIRDNLIPKEVYILLNWLGYVNSAFNPLIYCRSPDFRIAFQELLCLRRSSSKTY
GNGYSSNSNGRTDYTGEPNTCQLGQEREQELLCEDPPGMEGFVNCQGTVPSLSVDSQGRNCSTNDSPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007420
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007420.3
RefSeq Size 2269 bp
RefSeq ORF 1257 bp
Locus ID 11555
UniProt ID P18762
MW 47 kDa
Gene Summary This intronless gene belongs to the G-protein-coupled receptor superfamily, which includes transmembrane proteins that play a role in signal transduction across biological membranes resulting in a variety of physiological responses. The encoded protein is a beta-2 adrenergic receptor which is activated by catecholamine ligands such as adrenaline and epinephrine. The protein participates in the classical signaling pathway involving G protein, adenylyl cyclase, cAMP (3'-5'-cyclic adenosine monophosphate) and protein kinase A (PKA). In humans, this gene is implicated in susceptibility to nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Apr 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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