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Nelfe (NM_001045863) Mouse Tagged ORF Clone
CAT＃: MR205656
- TrueORF®
Nelfe (Myc-DDK-tagged) - Mouse RD RNA-binding protein (Rdbp), transcript variant 2
"NM_001045863" in other vectors (2)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D17H6S45; NELF-E; RD; Rdbp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205656 representing NM_001045863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTGATACCTCCGGGGCTGAGTGAAGAGGAAGAGGCACTGCAGAAGAAGTTCAACAAACTCAAGA AGAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAGAGCAGCAGCGGCCCAGCGAGCCAGGGCGGTGTCAA ACGCTCACTGTCAGAGCAGCCTGTGGTGGACACTGCCACAGCAACAGAACAGGCCAAACAACTGGTGAAG TCAGGAGCCATCAGTGCCATCAAGGCTGAAACTAAGAACTCAGGCTTCAAACGTTCTCGGACCCTGGAAG GGAAGTTAAAGGACCCAGAGAAGGGGCCTGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATGTCTGCTGA TGAGGACCTACAGGAGCCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAAAGCTTTGTGTCTTCTAGT GATCGGCTTCGGGAACTGGGACAAGATGGAGAAGAAGCAGAGGCCCCCGGGGCTGGTGATGGCCCACCTC GAGGCTTTGACTGGAGCTATGAAGAGCATGGTAGCGCCCGCTCCTCAGCCTCCCCTCCCCGAAGCCGCAG CAGGGACCGAAGTCATGACCGGAGCCGAGATAGGGATCGGGACAAAGAGAGAGACAGAGACCGAGATCGA GATCGAGACAGAGACCGGGATAAAGACAAAGACAGAGACCGGGATCGAGACAGAGACCGGGACCGAGATC GGGAACGAGACCGAGAGGGCCCTTTCCGCAGGTCAGACTCATTCCCCGAACGGAGGGCCCCTCGAAAGGG GAACACACTGTATGTGTATGGAGAGGACATGACGCCCACCCTCCTCCGCGGGGCCTTCTCTCCCTTTGGA AATATCATCGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTCACCTACGAGAAGATGGAGTCTG CAGATCAGGCTGTTGCTGAGCTCAATGGGACCCAGGTGGAATCTGTGCAGCTTAAAGTCAACATAGCCCG AAAACAGCCCATGCTGGACGCTGCTACTGGCAAATCTGTCTGGGGCTCCCTTGCTGTCCAGAACAGCCCT AAGGGTTGCCATCGAGACAAGAGGACCCAGATTGTGTACAGTGACGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205656 representing NM_001045863
Red=Cloning site Green=Tags(s) MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGPASQGGVKRSLSEQPVVDTATATEQAKQLVK SGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSS DRLRELGQDGEEAEAPGAGDGPPRGFDWSYEEHGSARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDR DRDRDRDKDKDRDRDRDRDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFG NIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSP KGCHRDKRTQIVYSDDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001045863 |
| ORF Size | 1101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001045863.1, NP_001039328.1 |
| RefSeq Size | 1535 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 27632 |
| UniProt ID | P19426 |
| MW | 41.9 kDa |
| Gene Summary | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR205656L3 | Lenti ORF clone of Nelfe (Myc-DDK-tagged) - Mouse RD RNA-binding protein (Rdbp), transcript variant 2 |
CNY 4,750.00 |
|
| MR205656L4 | Lenti ORF clone of Nelfe (mGFP-tagged) - Mouse RD RNA-binding protein (Rdbp), transcript variant 2 |
CNY 4,750.00 |
