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Pdhb (NM_024221) Mouse Tagged ORF Clone
CAT＃: MR205484
- TrueORF®
Pdhb (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (Pdhb), nuclear gene encoding mitochondrial protein
"NM_024221" in other vectors (3)
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CNY 2,850.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610103L06Rik; AL024199; C81408 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205484 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGTGGCTGGTCTGGTGCGGGGACCCTTGCGGCAGGCTTCCGGGCTGCTGAAGAGGCGTTTTC ACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATCAAGGTATGGATGAAGAACT AGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGC AGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCATCGACACCCCCATCTCAGAGATGGGCTTTGCTG GAATTGCTGTTGGTGCAGCTATGGCTGGGTTGCGGCCTATCTGTGAATTTATGACCTTCAATTTCTCTAT GCAAGCTATTGACCAGGTTATAAACTCAGCTGCCAAGACTTACTATATGTCCGCTGGCCTTCAGCCTGTG CCCATAGTATTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCTCAGCACTCGCAATGCTTTGCTG CATGGTATGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATGCAAAAGGACTTAT TAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGAAAATGAACTGATGTATGGAGTTGCATTT GAACTTCCTGCAGAAGCTCAGTCAAAAGATTTCCTGATTCCTATTGGAAAAGCCAAAATTGAAAGGCAAG GGACCCACATCACTGTAGTTGCCCATTCAAGACCAGTGGGCCACTGCCTAGAAGCTGCAGCTGTATTGTC TAAGGAGGGAATTGAATGTGAGGTAATAAATCTGCGCACCATCAGACCAATGGACATCGAAGCCATAGAA GCCAGTGTGATGAAGACAAACCATCTCGTGACTGTGGAAGGAGGCTGGCCACAATTTGGAGTAGGAGCTG AGATTTGTGCCAGAATTATGGAAGGCCCTGCATTCAACTTCCTTGATGCCCCTGCCGTGCGTGTCACTGG TGCTGATGTCCCTATGCCTTACGCAAAGGTCCTAGAAGACAACTCCGTACCTCAGGTCAAAGACATCATA TTTGCAGTAAAGAAGACTCTGAACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205484 protein sequence
Red=Cloning site Green=Tags(s) MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMDEELERDEKVFLLGEEVAQYDGAYKVS RGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPV PIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAF ELPAEAQSKDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIE ASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDII FAVKKTLNI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024221 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024221.1, NM_024221.2, NM_024221.3, NP_077183.1 |
| RefSeq Size | 1485 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 68263 |
| UniProt ID | Q9D051 |
| MW | 38.9 kDa |
| Gene Summary | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
,null,
Cell metabolism
,PubMed ID 35305295
[Pdhb]
|
|
Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
,Terayama, H;Hirai, S;Naito, M;Qu, N;Katagiri, C;Nagahori, K;Hayashi, S;Sasaki, H;Moriya, S;Hiramoto, M;Miyazawa, K;Hatayama, N;Li, ZL;Sakabe, K;Matsushita, M;Itoh, M;,
Sci Rep
,PubMed ID 27752123
[PDHB]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201236 | Pdhb (untagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (Pdhb), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR205484L3 | Lenti ORF clone of Pdhb (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (Pdhb), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR205484L4 | Lenti ORF clone of Pdhb (mGFP-tagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (Pdhb), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
