Dcun1d5 (NM_029775) Mouse Tagged ORF Clone
CAT#: MR202903
- TrueORF®
Dcun1d5 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5)
ORF Plasmid: tGFP
"NM_029775" in other vectors (4)
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CNY 1,416.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3110001A18Rik; 4833420K19Rik; AW060460; D430047L21Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202903 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGAAGAAGAAGAGAAAAGCTCCCGGAGTGGCAGCGGCGGTCGCGGAAGACGCGGGCCTCAAAA AGTGTAAAATCCCCAGCTATTGCAGATCCCAGCCTCCTGCTAGACTCATCAGTGGAGAGGAAGATTTTTC AAGAAAGAAGTGCCTGGCTTGGTTTTATGAGTATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATG GAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAACATTATTATGTTAGTTTTAGCATGGAAATTGG AGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACCTCATTACAGTGTGACTG CACAGAAAAGTTACAGAGCAGGTTTGATTTTCTGCGCTCTCAGCTGAATGACATCTCATCTTTTAAGAAT ATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCGAAGT CTATGTTAGCTCTGCTGCTTGGGAGAACATGGCCACTCTTTTCAGTATTTTACCAGTACCTGGAGCAATC AAAGTATCGTGTTATGAACAAAGATCAGTGGTACAATGTATTAGAATTCAGCAGAACAGTTCATGCCGAT CTTAGTAACTACGATGAAGACGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAATCC GTCAAACATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202903 protein sequence
Red=Cloning site Green=Tags(s) MPVKKKRKAPGVAAAVAEDAGLKKCKIPSYCRSQPPARLISGEEDFSRKKCLAWFYEYAGPDEVVGPEGM EKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQSRFDFLRSQLNDISSFKN IYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHAD LSNYDEDGAWPVLLDEFVEWQKIRQTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_029775 |
ORF Size | 714 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029775.3 |
RefSeq Size | 1201 bp |
RefSeq ORF | 714 bp |
Locus ID | 76863 |
UniProt ID | Q9CXV9 |
MW | 27.6 kDa |
Gene Summary | Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs). May play a role in DNA damage response and may participate to cell proliferation and anchorage-independent cell growth.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition
,Yang, Y;Zhou, C;Wang, Y;Liu, W;Liu, C;Wang, L;Liu, Y;Shang, Y;Li, M;Zhou, S;Wang, Y;Zeng, W;Zhou, J;Huo, R;Li, W;,
EMBO Rep
,PubMed ID 28073917
[DCUN1D5]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203127 | Dcun1d5 (untagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5), (10ug) |
CNY 2,000.00 |
|
MG202903 | Dcun1d5 (tGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 2,850.00 |
|
MR202903L3 | Lenti ORF clone of Dcun1d5 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 4,750.00 |
|
MR202903L4 | Lenti ORF clone of Dcun1d5 (mGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5) |
CNY 4,750.00 |
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