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Cd28 (NM_007642) Mouse Tagged ORF Clone
CAT＃: MR202454
- TrueORF®
Cd28 (Myc-DDK-tagged) - Mouse CD28 antigen (Cd28)
ORF Plasmid: tGFP
"NM_007642" in other vectors (6)
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CNY 2,400.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202454 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCAGGCTGCTGTTCTTGGCTCTCAACTTCTTCTCAGTTCAAGTAACAGAAAACAAGATTTTGG TAAAGCAGTCGCCCCTGCTTGTGGTAGATAGCAACGAGGTCAGCCTCAGCTGCAGGTATTCCTACAACCT TCTCGCAAAGGAATTCCGGGCATCCCTGTACAAGGGCGTGAACAGCGACGTGGAAGTCTGTGTCGGGAAT GGGAATTTTACCTATCAGCCCCAGTTTCGCTCGAATGCCGAGTTCAACTGCGACGGGGATTTCGACAACG AAACAGTGACGTTCCGTCTCTGGAATCTGCACGTCAATCACACAGATATTTACTTCTGCAAAATTGAGTT CATGTACCCTCCGCCTTACCTAGACAACGAGAGGAGCAATGGAACTATTATTCACATAAAAGAGAAACAT CTTTGTCATACTCAGTCATCTCCTAAGCTGTTTTGGGCACTGGTCGTGGTTGCTGGAGTCCTGTTTTGTT ATGGCTTGCTAGTGACAGTGGCTCTTTGTGTTATCTGGACAAATAGTAGAAGGAACAGACTCCTTCAAAG TGACTACATGAACATGACTCCCCGGAGGCCTGGGCTCACTCGAAAGCCTTACCAGCCCTACGCCCCTGCC AGAGACTTTGCAGCGTACCGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202454 protein sequence
Red=Cloning site Green=Tags(s) MTLRLLFLALNFFSVQVTENKILVKQSPLLVVDSNEVSLSCRYSYNLLAKEFRASLYKGVNSDVEVCVGN GNFTYQPQFRSNAEFNCDGDFDNETVTFRLWNLHVNHTDIYFCKIEFMYPPPYLDNERSNGTIIHIKEKH LCHTQSSPKLFWALVVVAGVLFCYGLLVTVALCVIWTNSRRNRLLQSDYMNMTPRRPGLTRKPYQPYAPA RDFAAYRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007642 |
| ORF Size | 657 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007642.4 |
| RefSeq Size | 4317 bp |
| RefSeq ORF | 657 bp |
| Locus ID | 12487 |
| UniProt ID | P31041 |
| MW | 25.2 kDa |
| Gene Summary | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inclusion of Dap10 or 4-1BB costimulation domains in the chPD1 receptor enhances anti-tumor efficacy of T cells in murine models of lymphoma and melanoma
,Kintz, H;Nylen, E;Barber, A;,
Cell. Immunol.
,PubMed ID 32106933
[CD28]
|
|
Adoptive transfer of murine T cells expressing a chimeric-PD1-Dap10 receptor as an immunotherapy for lymphoma
,Lynch, A;Hawk, W;Nylen, E;Ober, S;Autin, P;Barber, A;,
Immunology
,PubMed ID 28670716
[CD28]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208000 | Cd28 (untagged) - Mouse CD28 antigen (Cd28), (10ug) |
CNY 3,600.00 |
|
| MG202454 | Cd28 (tGFP-tagged) - Mouse CD28 antigen (Cd28) |
CNY 4,000.00 |
|
| MR202454L1 | Lenti ORF clone of Cd28 (Myc-DDK-tagged) - Mouse CD28 antigen (Cd28) |
CNY 4,800.00 |
|
| MR202454L2 | Lenti ORF clone of Cd28 (mGFP-tagged) - Mouse CD28 antigen (Cd28) |
CNY 4,800.00 |
|
| MR202454L3 | Lenti ORF clone of Cd28 (Myc-DDK-tagged) - Mouse CD28 antigen (Cd28) |
CNY 4,800.00 |
|
| MR202454L4 | Lenti ORF clone of Cd28 (mGFP-tagged) - Mouse CD28 antigen (Cd28) |
CNY 4,800.00 |
