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Rala (NM_019491) Mouse Tagged ORF Clone
CAT＃: MR202179
- TrueORF®
Rala (Myc-DDK-tagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala)
ORF Plasmid: tGFP
"NM_019491" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3010001O15Rik; AW322615; Ral; Rasl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202179 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAAACAAGCCCAAGGGTCAGAATTCTTTGGCCTTACACAAAGTCATCATGGTGGGCAGTGGTG GTGTGGGCAAGTCTGCCCTGACTCTGCAGTTCATGTACGACGAGTTTGTAGAGGACTATGAACCTACCAA AGCGGACAGCTACAGGAAGAAGGTAGTCCTGGACGGGGAAGAAGTGCAGATCGACATCTTAGATACAGCG GGGCAGGAGGACTATGCTGCAATTAGAGACAACTACTTCCGAAGTGGGGAGGGATTCCTCTGTGTCTTCT CTATCACAGAGATGGAGTCCTTCGCAGCTACCGCGGACTTCAGGGAGCAGATTTTAAGAGTAAAAGAAGA TGAGAATGTTCCATTTCTCCTGGTTGGTAACAAGTCAGATCTAGAAGATAAAAGGCAGGTTTCTGTAGAA GAGGCAAAAAACAGAGCTGACCAGTGGAACGTTAACTATGTGGAGACGTCTGCTAAAACGCGCGCCAACG TTGACAAGGTATTTTTTGATTTAATGAGGGAAATACGAGCCAGAAAGATGGAAGACAGCAAAGAAAAAAA TGGAAAAAAGAAGAGGAAAAGTTTAGCCAAGAGAATCAGAGAAAGATGCTGCATTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202179 protein sequence
Red=Cloning site Green=Tags(s) MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTA GQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQVSVE EAKNRADQWNVNYVETSAKTRANVDKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERCCIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019491 |
| ORF Size | 621 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019491.5, NP_062364.3 |
| RefSeq Size | 2662 bp |
| RefSeq ORF | 621 bp |
| Locus ID | 56044 |
| UniProt ID | P63321 |
| MW | 23.6 kDa |
| Gene Summary | Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201478 | Rala (untagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala), (10ug) |
CNY 2,000.00 |
|
| MG202179 | Rala (tGFP-tagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala) |
CNY 2,850.00 |
|
| MR202179L3 | Lenti ORF clone of Rala (Myc-DDK-tagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala) |
CNY 4,750.00 |
|
| MR202179L4 | Lenti ORF clone of Rala (mGFP-tagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala) |
CNY 4,750.00 |
