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Desi2 (NM_024282) Mouse Tagged ORF Clone
CAT＃: MR201898
- TrueORF®
Desi2 (Myc-DDK-tagged) - Mouse PPPDE peptidase domain containing 1 (Pppde1)
ORF Plasmid: tGFP
"NM_024282" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5830417C01Rik; DeSI-2; Fam152a; Pppde1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201898 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCTAACCAGTTAGTGGTGCTCAACGTCTACGACATGTACTGGATGAATGAATACACCTCATCTA TTGGAATTGGAGTTTTTCATTCTGGAATTGAAGTATATGGCAGAGAGTTTGCTTATGGTGGCCATCCATA TCCTTTTTCTGGAATATTTGAAATTTCCCCAGGAAATGCTTCTGAGCTAGGAGAAACATTTAAATTTAAA GAAGCTGTTGTTCTAGGAAGTACGGACTTTCTAGAAGATGATATAGAGAAAATTGTAGAAGAACTGGGGA AAGAGTATAAGGGCAACGCCTACCATCTGATGCACAAAAACTGCAATCACTTTTCTTCAGCTTTATCAGA GATTCTCTGTGGGAAAGAGATTCCTCGCTGGATCAACCGGCTGGCCTACTTCAGCTCCTGTATACCCTTT CTACAGAGTTGCCTGCCAAAGGAGTGGCTCACTCCTGCTGCACTTCAGTCTAGTGTCAGCCAAGAACTCC AGGATGAACTAGAAGAAGCAGAGGATGCAGCCGCATCCAGTGCCATGGCAAGTGCTGCAGCTGGTGCCAG AACTGGGCGTCACACTAAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201898 protein sequence
Red=Cloning site Green=Tags(s) MGANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFK EAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPF LQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASSAMASAAAGARTGRHTKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024282 |
| ORF Size | 585 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024282.3, NP_077244.1 |
| RefSeq Size | 4214 bp |
| RefSeq ORF | 585 bp |
| Locus ID | 78825 |
| UniProt ID | Q9D291 |
| MW | 21.4 kDa |
| Gene Summary | Has deubiquitinating activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Deubiquitinates 'Lys-48'-linked polyubiquitination of RPS7 leading to its stabilization.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200072 | Desi2 (untagged) - Mouse PPPDE peptidase domain containing 1 (Pppde1), (10ug) |
CNY 2,000.00 |
|
| MG201898 | Desi2 (tGFP-tagged) - Mouse RIKEN cDNA 5830417C01 gene (5830417C01Rik) |
CNY 2,850.00 |
|
| MR201898L3 | Lenti ORF clone of Desi2 (Myc-DDK-tagged) - Mouse PPPDE peptidase domain containing 1 (Pppde1) |
CNY 4,750.00 |
|
| MR201898L4 | Lenti ORF clone of Desi2 (mGFP-tagged) - Mouse PPPDE peptidase domain containing 1 (Pppde1) |
CNY 4,750.00 |
