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Sptssa (NM_134054) Mouse Tagged ORF Clone
CAT＃: MR200062
- TrueORF®
Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik)
ORF Plasmid: tGFP
"NM_134054" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110002B05Rik; AA407909; AA409588; AU041967; Ssspta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200062 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGGCGCGAGCATGGAAGCAGATGTCCTGGTTCTACTACCAGTACCTGCTGGTCACTGCGCTCT ACATGCTGGAGCCCTGGGAGCGAACCGTGTTCAATTCGATGCTGGTTTCCGTGGTGGGGATGGCCCTGTA CACTGGCTACGTCTTCATGCCCCAGCACATCATGGCTATTCTGCATTACTTTGAAATTGTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200062 protein sequence
Red=Cloning site Green=Tags(s) MALARAWKQMSWFYYQYLLVTALYMLEPWERTVFNSMLVSVVGMALYTGYVFMPQHIMAILHYFEIVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134054 |
| ORF Size | 207 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134054.1 |
| RefSeq Size | 1363 bp |
| RefSeq ORF | 216 bp |
| Locus ID | 104725 |
| UniProt ID | Q8R207 |
| MW | 8.2 kDa |
| Gene Summary | Stimulates the activity of serine palmitoyltransferase (SPT). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. Plays a role in MBOAT7 location to mitochondria-associated membranes (MAMs), may me involved in fatty acid remodeling phosphatidylinositol (PI).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207810 | Sptssa (untagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik), (10ug) |
CNY 3,990.00 |
|
| MG200062 | Sptssa (tGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) |
CNY 2,850.00 |
|
| MR200062L1 | Lenti ORF clone of Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) |
CNY 3,600.00 |
|
| MR200062L2 | Lenti ORF clone of Sptssa (mGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) |
CNY 4,750.00 |
|
| MR200062L3 | Lenti ORF clone of Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) |
CNY 4,750.00 |
|
| MR200062L4 | Lenti ORF clone of Sptssa (mGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) |
CNY 4,750.00 |
