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Rap1b (NM_024457) Mouse Tagged ORF Clone
CAT＃: MG227658
- TrueORF®
Rap1b (tGFP-tagged) - Mouse RAS related protein 1b (Rap1b), (10ug)
ORF Plasmid: DDK
"NM_024457" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810443E11Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227658 representing NM_024457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGAATATAAGCTCGTCGTGCTTGGATCAGGAGGCGTTGGGAAGTCAGCCCTGACTGTACAGTTTG TTCAAGGAATTTTTGTTGAAAAATATGATCCTACGATAGAAGATTCTTATAGAAAGCAAGTTGAAGTAGA TGCACAGCAGTGTATGCTTGAAATCTTGGACACTGCAGGAACGGAGCAATTCACAGCCATGAGGGACCTG TACATGAAGAACGGACAAGGCTTCGCTCTGGTTTACTCCATCACAGCACAGTCGACATTTAACGACTTAC AAGATCTAAGAGAGCAGATTCTGCGGGTTAAAGACACTGATGATGTTCCAATGATTCTGGTTGGCAATAA ATGCGACTTGGAAGATGAAAGAGTTGTAGGAAAGGAACAAGGTCAGAACCTAGCAAGACAGTGGAACAAC TGTGCATTCTTAGAATCCTCTGCAAAGTCCAAAATAAATGTTAATGAGATCTTTTATGACCTAGTGCGGC AAATTAACAGAAAAACTCCAGTGCCTGGGAAGGCCCGCAAAAAGTCATCGTGTCAGCTGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227658 representing NM_024457
Red=Cloning site Green=Tags(s) MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDL YMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNN CAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024457 |
| ORF Size | 552 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024457.2, NP_077777.1 |
| RefSeq Size | 1937 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 215449 |
| UniProt ID | Q99JI6 |
| Gene Summary | GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212622 | Rap1b (untagged) - Mouse RAS related protein 1b (Rap1b), (10ug) |
CNY 3,990.00 |
|
| MR227658 | Rap1b (Myc-DDK-tagged) - Mouse RAS related protein 1b (Rap1b) |
CNY 2,400.00 |
|
| MR227658L3 | Lenti ORF clone of Rap1b (Myc-DDK-tagged) - Mouse RAS related protein 1b (Rap1b) |
CNY 4,750.00 |
|
| MR227658L4 | Lenti ORF clone of Rap1b (mGFP-tagged) - Mouse RAS related protein 1b (Rap1b) |
CNY 4,750.00 |
