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Dscam (NM_031174) Mouse Tagged ORF Clone
CAT＃: MG227575
- TrueORF®
Dscam (tGFP-tagged) - Mouse Down syndrome cell adhesion molecule (Dscam), (10ug)
ORF Plasmid: DDK
"NM_031174" in other vectors (2)
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CNY 21,536.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4932410A21Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227575 representing NM_031174
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGAGCCCCACTCCAGCC TCTACTTTGTCAATGCATCGCTGCAAGAGGTAGTGTTTGCAAGCACATCGGGGACGCTGGTGCCCTGCCC GGCTGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCAACGGGCGAGGAGATCTACGATGTCCCC GGGATCCGCCACGTCCATCCCAATGGCACTCTCCAAATTTTCCCCTTTCCTCCTTCAAGCTTCAGCACCT TAATCCATGATAATACTTACTATTGCACAGCTGAAAACCCTTCAGGGAAAATTAGAAGTCAGGATGTCCA CATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTC GCGGTGTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACGTCACTGTCGTCTCATGGGAGAAAGACA CGGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAAGATGTACA GAACGAAGATGGGCTGTACAACTACCGCTGCATCACGCGGCACAGATACACAGGGGAGACGAGACAAAGC AACAGCGCGAGACTGTTCGTGTCAGACCCAGCAAACTCAGCCCCATCCATCCTGGACGGGTTTGACCACC GCAAAGCCATGGCAGGGCAGCGCGTGGAGCTGCCTTGCAAAGCACTTGGGCACCCCGAGCCAGACTACCG CTGGCTGAAGGACAACATGCCCCTGGAACTTTCTGGAAGGTTCCAAAAGACAGTGACTGGGTTGCTCATT GAGAACAGCCGCCCCTCAGACTCAGGCAGCTATGTGTGTGAAGTATCCAACCGATATGGCACTGCCAAGG TGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGAAAGGTTAAAAGCAGCGT GGGCAGCCAGGTCTCCTTATCCTGCAGTGTGACAGGAAATGAAGACCAGGAACTCTCCTGGTACCGAAAT GGCGAAATCCTCAACCCTGGAAAAAACGTGAGGATCACAGGACTCAACCACGCAAACCTTATAATGGATC ACATGGTCAAGAGTGATGGGGGTGCCTACCAGTGCTTTGTGCGCAAGGACAAGCTATCTGCTCAAGACTA TGTCCAGGTGGTCCTTGAAGACGGAACTCCCAAAATCATTTCTGCCTTTAGCGAGAAAGTGGTGAGCCCG GCAGAGCCAGTGTCCCTCGTGTGCAATGTGAAGGGTACACCCTTGCCCACGGTCACCTGGACCCTGGACG ATGACCCCATCCTCAAGGGCAGCGGTCACCGCATCAGCCAAATGATCACGTCCGAGGGGAACGTGGTCAG CTACCTGAATATCTCCAGCTCCCAGGTCCGGGATGGGGGTGTCTACCGCTGCACTGCCAACAACTCGGCT GGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCTGCAAGCATCAGACCAATGAAAAACATCA CTGCGATAGCGGGGCGTGACACGTACATCCACTGCCGCGTAATTGGCTATCCGTATTACTCCATCAAGTG GTACAAGAACGCTAACCTGCTTCCTTTCAACCACCGCCAGGTGGCGTTTGAGAACAATGGGACTCTGAAA CTCTCTGATGTGCAGAAAGAAGTTGACGAGGGAGAGTACACGTGTAATGTGCTGGTACAGCCACAGCTCT CCACCAGCCAGAGTGTCCACGTGACAGTGAAAGTTCCACCTTTCATCCAACCTTTTGAGTTCCCAAGATT CTCCATCGGGCAGCGGGTTTTCATCCCATGTGTGGTGGTCTCAGGGGACTTACCCATCACCATCACCTGG CAGAAGGATGGCCGGCCAATTCCAGCAAGCCTCGGAGTAACCATTGACAACATTGACTTCACCAGCTCCC TGAGGATCTCCAACCTCTCCCTAATGCACAATGGGAATTACACCTGCATTGCGAGAAACGAGGCAGCAGC CGTGGAACACCAGAGTCAGCTGATTGTGAGAGTTCCCCCTAAGTTCGTGGTACAGCCCCGGGACCAGGAC GGGATCTATGGCAAAGCAGTGATTCTCAATTGCTCTGCAGAGGGTTATCCTGTGCCTACAATTGTGTGGA AATTCTCAAAAGGTGCTGGGGTCCCCCAGTTCCAGCCAATTGCCTTGAATGGCCGAATCCAGGTCCTGAG TAATGGCTCACTCTTGATCAAGCATGTTGTAGAAGAAGACAGTGGCTACTACCTCTGCAAGGTCAGCAAC GATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTCACAGTGAAAATTCCTGCCATGATAACCTCTTACC CCAACACCACCCTGGCCACTCAGGGTCAAAGGAAGGAAATGAGCTGCACAGCCCATGGGGAGAAGCCCAT CATTGTCCGCTGGGAGAAAGAGGACAGGATCATTAACCCTGAAATGGCCCGCTACCTGGTATCCACCAAG GAGGTGGGAGAGGAAGTGATATCTACGCTGCAGATTTTGCCAACAGTGAGAGAAGATTCCGGTTTCTTCT CCTGCCATGCTATCAATTCATACGGGGAGGACCGTGGAATAATTCAACTCACAGTGCAAGAACCCCCAGA TCCTCCCGAGATTGAGATCAAAGATGTCAAAGCTCGCACCATCACGCTCAGGTGGACCATGGGGTTTGAT GGCAACAGCCCCATCACAGGCTATGACATTGAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAAA GAACCAAAGATGTTTCCCCTCAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAG CATCCGCATGTACGCCAAGAACCGGATTGGCAAGAGTGAGCCCAGCAACGAGATCACCATCACGGCGGAT GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTTCACTTGGAACCCACCTCATCTCAGAGTATCAGGGTTA CCTGGAAGGCTCCCAAGAAACACTTACAAAACGGGATCATTCGCGGCTACCAAATAGGCTACCGGGAGTA CAGCACGGGGGGTAACTTCCAGTTCAACATCATCAGTATCGACACCACCGGGGACAGCGAAGTGTACACC CTGGACAACCTGAATAAGTTCACGCAGTATGGCCTGGTAGTACAGGCTTGCAACCGGGCCGGCACAGGAC CTTCTTCTCAGGAGATCATTACCACCACTCTGGAGGATGTACCCAGCTACCCTCCTGAAAATGTCCAAGC CATAGCAACCTCACCAGAAAGCATATCAATCTCCTGGTCCACACTGTCCAAGGAGGCCTTGAATGGGATT CTCCAGGGGTTCAGGGTCATCTACTGGGCCAACCTCATAGACGGAGAGCTGGGCGAGATTAAGAACGTCA CCACCACGCAGCCTTCCTTGGAGTTGGATGGACTAGAGAAATACACCAACTACAGTATCCAGGTCCTGGC CTTCACCCGTGCAGGGGATGGCGTCCGGAGCGAGCAGATCTTTACCCGTACCAAGGAGGACGTTCCAGGT CCTCCTGCCGGTGTCAAGGCGGCGGCAGCCTCGGCCTCCATGGTCTTCGTGTCCTGGCTGCCCCCGCTGA AGCTGAACGGCATCATTCGGAAGTACACAGTGTTCTGCTCCCATCCCTACCCTACCGTCATCAGTGAGTT TGAAGCCTCCCCTGACTCATTTTCCTACAGAATCCCTAATCTGAGTCGGAATCGGCAGTATAGCGTCTGG GTGGTGGCGGTGACTTCCGCCGGAAGAGGCAACAGCAGCGAGATCATCACTGTGGAGCCCCTAGCTAAAG CTCCTGCACGGATCCTCACGTTCAGCGGGACAGTGACTACTCCATGGATGAAGGACATTGTCTTGCCTTG TAAAGCTGTTGGAGACCCGTCACCTGCCGTAAAATGGATGAAGGATAGTAACGGGACACCCAGCCTGGTG ACGATTGATGGCCGCAGGAGCATCTTCAGCAATGGGAGTTTCATCATTCGGACGGTGAAAGCAGAGGACT CTGGCTATTACAGCTGTGTTGCCAATAACAACTGGGGATCAGATGAGATCATATTAAATTTGCAAGTACA AGTTCCACCAGATCAGCCTCGGCTCACCGTATCCAAGACGACATCCTCCTCCATCACCCTCTCCTGGCTC CCTGGAGATAATGGGGGTAGCTCCATTAGAGGCTACATCCTGCAATACTCCGAGGATAACAGTGAGCAGT GGGGCAGCTTTCCCATCAGCCCCAGCGAGCGTTCATACCGCTTGGAAAACCTAAAGTGTGGGACTTGGTA TAAGTTCACCCTTACTGCCCAAAATGGAGTAGGTCCCGGGCGCATAAGTGAAATCATAGAAGCCAAAACC CTGGGGAAAGAACCCCAGTTCTCCAAGGAGCAGGAGCTCTTCGCCAGCATCAATACCACCCGAGTGAGGC TGAATCTGATTGGCTGGAATGACGGCGGCTGTCCAATCACCTCATTCACTCTTGAATACAGACCCTTTGG GACCACGGTCTGGACCACAGCTCAGCGGACCTCCCTTTCCAAGTCCTACATTCTGTATGACCTGCAAGAA GCCACGTGGTATGAACTGCAGATGAGAGTGTGCAACAGCGCCGGCTGTGCGGAGAAGCAAGCCAACTTCG CCACGCTGAACTACGATGGCAGTACAATCCCTCCACTCATTAAGTCAGTTGTCCAAAGCGAAGAAGGGCT GACAACCAACGAAGGGCTCAAGATCCTCGTGACCATCTCCTGCATCCTGGTCGGGGTTCTACTGCTCTTT GTGCTTCTGCTGGTTGTGCGGAGGAGACGGCGAGAGCAGAGGCTGAAGAGGCTGAGAGATGCAAAGAGTT TAGCTGAAATGCTCATGAGCAAAAACACACGGACTTCAGATACCTTAAGCAAACAGCAGCAGACTTTGAG AATGCACATTGATATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACAATGGAGACCATAGATGAC CGCTCCACAGTCCTGTTGACGGATGCTGACTTCGGGGAGGCAGCCAAACAGAAGTCACTGACAGTGACTC ACACGGTGCATTACCAATCGGTGTCTCAGGCCACCGGGCCCCTCGTGGATGTCTCCGATGCTCGGCCAGG AACGAATCCCACCACCAGGAGGAATGCAAAGGCTGGACCCACAGCGAGAAACCGGTACGCCAGCCAGTGG ACGCTCAACAGACCCCATCCTACCATCTCTGCACACACCCTCACCACAGACTGGAGACTGCCTACACCCA GGGCTACAGGATCCGTGGACAAGGAGAGCGACAGCTACAGCGTCAGCCCATCACAAGACACAGACCGAGC AAGAAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCTACCTACGAAGAACTGGCCAGGGCCTATGAACAC GCCAAGATGGAAGAGCAGCTGAGGCATGCCAAGTTCACCATCACAGAGTGCTTCATATCCGATACGTCCT CCGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCCAGTACCCCTTCAGAATCGGG GATCTGCAGATTCACTGCATCTCCCCCCAAACCTCAGGATGGAGGACGAGTGGTGAACATGGCGGTTCCA AAGGCCCATCGGCCAGGCGACCTCATACACCTGCCTCCATACCTACGAATGGACTTCTTGTTAAACCGGG GCGCACCAGGCACCAGCAGGGACCTGAGTTTAGGACAAGCGTGCTTGGAACCCCAGAAAAGTCGGACCCT GAAACGCCCCACGGTCCTTGAGCCCACCCCTATGGAGGCCTCCTCCTCCACCTCTTCCACGCGAGAAGGA CAGCAGTCGTGGCAACAAGGGGCTGTGGCCACCTTACCTCAGCGAGAGGGTGCAGAGCTGGGACAGGCAG CTAAAATGAGCAGCTCCCAAGAGTCACTGCTGGACTCCCGGGGCCATTTGAAAGGAAACAATCCCTACGC AAAATCTTACACCTTGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227575 representing NM_031174
Red=Cloning site Green=Tags(s) MWILALSLFQSFANVFSEEPHSSLYFVNASLQEVVFASTSGTLVPCPAAGIPPVTLRWYLATGEEIYDVP GIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKAVLREPYTVRVEDQKTMRGNV AVFKCIIPSSVEAYVTVVSWEKDTVSLVSGSRFLITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQS NSARLFVSDPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLI ENSRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGNEDQELSWYRN GEILNPGKNVRITGLNHANLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSP AEPVSLVCNVKGTPLPTVTWTLDDDPILKGSGHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSA GVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNANLLPFNHRQVAFENNGTLK LSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPFEFPRFSIGQRVFIPCVVVSGDLPITITW QKDGRPIPASLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQD GIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSN DVGADVSKSMYLTVKIPAMITSYPNTTLATQGQRKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTK EVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFD GNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPSNEITITAD EAAPDGPPQEVHLEPTSSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTGGNFQFNIISIDTTGDSEVYT LDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGI LQGFRVIYWANLIDGELGEIKNVTTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPG PPAGVKAAAASASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVW VVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWMKDSNGTPSLV TIDGRRSIFSNGSFIIRTVKAEDSGYYSCVANNNWGSDEIILNLQVQVPPDQPRLTVSKTTSSSITLSWL PGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLENLKCGTWYKFTLTAQNGVGPGRISEIIEAKT LGKEPQFSKEQELFASINTTRVRLNLIGWNDGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQE ATWYELQMRVCNSAGCAEKQANFATLNYDGSTIPPLIKSVVQSEEGLTTNEGLKILVTISCILVGVLLLF VLLLVVRRRRREQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDD RSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGPTARNRYASQW TLNRPHPTISAHTLTTDWRLPTPRATGSVDKESDSYSVSPSQDTDRARSSMVSTESASSTYEELARAYEH AKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTSSTPSESGICRFTASPPKPQDGGRVVNMAVP KAHRPGDLIHLPPYLRMDFLLNRGAPGTSRDLSLGQACLEPQKSRTLKRPTVLEPTPMEASSSTSSTREG QQSWQQGAVATLPQREGAELGQAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031174 |
| ORF Size | 6039 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031174.4, NP_112451.1 |
| RefSeq Size | 7481 bp |
| RefSeq ORF | 6042 bp |
| Locus ID | 13508 |
| UniProt ID | Q9ERC8 |
| Gene Summary | Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed:18216855, PubMed:19196994, PubMed:19945391). Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC (PubMed:18585357). Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC (PubMed:22685302). In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).[UniProtKB/Swiss-Prot Function] |
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