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Nuak2 (NM_001195025) Mouse Tagged ORF Clone
CAT＃: MG227560
- TrueORF®
Nuak2 (tGFP-tagged) - Mouse NUAK family SNF1-like kinase 2 (Nuak2) transcript variant A, (10ug)
ORF Plasmid: DDK
"NM_001195025" in other vectors (4)
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CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200013B22Rik; mKIAA0537; Omphk2; Snark |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227560 representing NM_001195025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGTGGCCTTACTCCAGCGCCCGAGCCAGGCTCCCTCGGCCTCCGCCCTGGCCTCGGAGAGCG CCCGGCCGCTGGCGGACGGGCTCATCAAGTCGCCTAAACCTCTGATGAAGAAGCAGGCGGTGAAGCGGCA CCATCACAAACACAACCTGCGGCACCGCTACGAGTTCCTGGAGACGCTGGGCAAGGGCACCTACGGGAAG GTGAAGAAGGCACGAGAGAGCTCGGGGCGTCTGGTGGCCATCAAGTCCATCAGGAAAGACAAAATCAAAG ATGAGCAGGATCTGCTGCACATACGGAGGGAGATTGAGATCATGTCTTCACTCAACCACCCCCACATCAT TGCCATCCATGAAGTGGGCAGATCACGCTTAGTGACTGTGTTTGAGAATAGCAGCAAGATTGTGATTGTC ATGGAGTATGCCAGCCGAGGCGATCTGTATGATTACATCAGTGAGCGGCCACGGCTGAGTGAGCGGGACG CCAGGCATTTCTTCCGACAGATCGTGTCTGCCCTGCACTACTGCCACCAGAACGGGATCGTTCACCGAGA TCTCAAGCTGGAAAACATCCTTCTAGATGCCAATGGAAACATCAAGATTGCTGACTTTGGCCTCTCCAAC CTGTACCACAAAGGCAAGTTCCTCCAGACGTTCTGTGGGAGCCCTCTCTACGCCTCGCCTGAGATAGTCA ACGGGAAGCCCTATGTGGGCCCAGAGGTGGACAGCTGGTCTCTGGGCGTTCTCCTGTACATCCTGGTGCA TGGCACCATGCCCTTTGACGGGCAGGATCATAAAACACTGGTGAAGCAAATCAGTAACGGGGCTTACCGT GAGCCGCCCAAGCCGTCCGATGCCTGTGGCCTGATCCGGTGGCTGTTAATGGTGAACCCCACCCGTCGGG CCACACTGGAGGATGTAGCCAGTCATTGGTGGGTCAACTGGGGTTACACCACCGGAGTCGGGGAACAGGA AGCCCTGCGTGAGGGTGGGCACCCTAGTGGTGACTTTGGCCGGGCCTCCATGGCGGACTGGTTACGTCGC TCCTCGCGCCCCCTCCTGGAGAATGGAGCCAAGGTGTGCAGCTTCTTCAAGCAGCACGTGCCGGGAGGTG GAAGCACTGTACCTGGGCTGGAGCGGCAACATTCTCTTAAGAAGTCCCGAAAGGAGAATGACATGGCTCA AAATCTGCAAGGTGACCCGGCTGAGGATACCTCTTCTCGCCCTGGCAAGAGCAGCCTTAAGCTTCCGAAA GGCATTCTCAAGAAAAAGTCCTCTACCTCGTCAGGGGAGGTACAGGAGGACCCTCAGGAACTCAGACCGG TGCCTGATACTCCAGGGCAGCCTGTCCCTGCTGTATCCCTGCTCCCAAGGAAAGGCATCCTTAAGAAGTC TCGACAGCGTGAATCTGGTTACTACTCCTCTCCAGAGCCCAGCGAGTCTGGGGAACTCTTAGACGCCAGT GATGTGTTTGTGAGTGGGGACCCCGTGGAGCAGAAGTCTCCACAGGCTTCAGGGCTCCTCCTCCACCGCA AGGGCATTCTCAAACTCAATGGCAAGTTCTCCCGCACAGCCTTAGAAGGCACTACCCCTAGCACCTTTGG CTCCCTGGACCAACTGGCCTCCTCCCATCCTGCAGCCCGGCCCAGCCGCCCCTCAGGGGCTGTGAGTGAG GACAGCATCCTGTCCTCCGAGTCCTTTGACCAATTGGACTTGCCTGAACGTCTTCCCGAAACCCCACTGA GGGGCTGTGTGTCTGTGGACAACCTGAGGGGGCTTGAGCAGCCTCCCTCAGAAGGTCTGAAGCGATGGTG GCAGGAATCCTTGGGGGATAGCTGCTTTTCTCTGACAGACTGCCAAGAGGTGACTGCAGCCTACAGACAA GCCCTAGGAATCTGCTCAAAGCTCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227560 representing NM_001195025
Red=Cloning site Green=Tags(s) MESVALLQRPSQAPSASALASESARPLADGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGK VKKARESSGRLVAIKSIRKDKIKDEQDLLHIRREIEIMSSLNHPHIIAIHEVGRSRLVTVFENSSKIVIV MEYASRGDLYDYISERPRLSERDARHFFRQIVSALHYCHQNGIVHRDLKLENILLDANGNIKIADFGLSN LYHKGKFLQTFCGSPLYASPEIVNGKPYVGPEVDSWSLGVLLYILVHGTMPFDGQDHKTLVKQISNGAYR EPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYTTGVGEQEALREGGHPSGDFGRASMADWLRR SSRPLLENGAKVCSFFKQHVPGGGSTVPGLERQHSLKKSRKENDMAQNLQGDPAEDTSSRPGKSSLKLPK GILKKKSSTSSGEVQEDPQELRPVPDTPGQPVPAVSLLPRKGILKKSRQRESGYYSSPEPSESGELLDAS DVFVSGDPVEQKSPQASGLLLHRKGILKLNGKFSRTALEGTTPSTFGSLDQLASSHPAARPSRPSGAVSE DSILSSESFDQLDLPERLPETPLRGCVSVDNLRGLEQPPSEGLKRWWQESLGDSCFSLTDCQEVTAAYRQ ALGICSKLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195025 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195025.1, NP_001181954.1 |
| RefSeq Size | 3132 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 74137 |
| UniProt ID | Q8BZN4 |
| Gene Summary | Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220016 | Nuak2 (untagged) - Mouse NUAK family, SNF1-like kinase, 2 (Nuak2), transcript variant A, (10ug) |
CNY 8,270.00 |
|
| MR227560 | Nuak2 (Myc-DDK-tagged) - Mouse NUAK family, SNF1-like kinase, 2 (Nuak2), transcript variant A |
CNY 6,370.00 |
|
| MR227560L3 | Lenti ORF clone of Nuak2 (Myc-DDK-tagged) - Mouse NUAK family, SNF1-like kinase, 2 (Nuak2), transcript variant A |
CNY 8,270.00 |
|
| MR227560L4 | Lenti ORF clone of Nuak2 (mGFP-tagged) - Mouse NUAK family, SNF1-like kinase, 2 (Nuak2), transcript variant A |
CNY 8,270.00 |
