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Rag1 (NM_009019) Mouse Tagged ORF Clone
CAT＃: MG227208
- TrueORF®
Rag1 (tGFP-tagged) - Mouse recombination activating gene 1 (Rag1), (10ug)
ORF Plasmid: DDK
"NM_009019" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Rag-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227208 representing NM_009019
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCCTTGCCGTCTACCCTGAGCTTCAGTTCTGCACCCGATGAAATTCAACACCCACAAATCA AATTTTCCGAGTGGAAATTTAAGCTGTTTAGGGTGAGATCCTTTGAAAAGGCACCCGAAGAAGCACAGAA GGAGAAGGATTCCTCAGAGGGGAAACCTTACCTAGAACAGTCTCCAGTAGTTCCAGAGAAGCCTGGTGGT CAGAACTCAATTCTGACTCAACGAGCACTGAAACTCCATCCTAAATTTTCAAAGAAATTCCATGCTGATG GGAAGTCAAGCGACAAAGCAGTTCACCAAGCCAGGCTTAGACACTTCTGCCGCATCTGTGGGAATCGTTT CAAGAGTGACGGGCACAGCCGGAGATACCCAGTCCACGGGCCCGTGGACGCTAAAACCCAAAGTCTTTTC CGAAAGAAGGAAAAAAGAGTCACTTCCTGGCCAGACCTCATTGCCAGGATTTTCCGGATCGACGTGAAGG CAGATGTTGACTCCATCCACCCGACGGAATTCTGCCATGACTGTTGGAGCATCATGCACAGAAAGTTCAG CAGTTCCCACAGTCAGGTCTACTTCCCAAGGAAAGTGACCGTGGAGTGGCACCCCCACACACCGTCCTGT GACATCTGTTTTACTGCCCATCGGGGACTCAAGAGGAAGAGACATCAGCCCAATGTGCAGCTCAGCAAGA AACTAAAAACTGTGCTCAACCACGCGAGACGGGACCGTCGCAAGAGAACTCAGGCTAGGGTCAGCAGCAA GGAAGTCCTGAAGAAGATCTCCAACTGCAGTAAGATTCATCTCAGTACCAAGCTTCTTGCCGTGGACTTC CCAGCACACTTTGTGAAATCCATCTCCTGCCAGATATGCGAACACATTCTGGCTGATCCCGTGGAGACCA GCTGCAAGCATCTATTCTGTAGGATCTGCATTCTCAGATGTCTCAAAGTCATGGGCAGCTATTGTCCCTC TTGCCGATATCCGTGCTTCCCTACTGACCTGGAGAGCCCAGTGAAGTCCTTTCTGAACATCTTGAATTCT CTCATGGTCAAGTGTCCCGCGCAAGATTGCAATGAGGAAGTGAGTCTGGAAAAATATAACCACCATGTGT CAAGCCACAAAGAATCTAAAGAGACTTTGGTGCATATCAATAAAGGGGGACGGCCTCGCCAGCATCTCCT GTCACTGACGAGAAGGGCGCAGAAACATCGGCTGAGGGAGCTCAAGATTCAAGTCAAAGAATTTGCTGAC AAAGAAGAAGGTGGAGATGTGAAGGCTGTCTGCTTGACATTGTTTCTCCTGGCACTGAGGGCGAGGAATG AGCACAGGCAAGCTGATGAATTAGAGGCCATCATGCAAGGCAGGGGCTCCGGGCTTCAACCAGCTGTTTG CTTGGCCATCCGTGTCAATACCTTCCTCAGCTGTAGCCAATACCATAAGATGTACAGGACTGTGAAAGCT ATCACTGGGAGGCAGATTTTTCAACCTTTGCATGCTCTTCGGAATGCCGAGAAAGTCCTTCTGCCAGGCT ACCATCCCTTTGAGTGGCAGCCCCCACTGAAGAATGTGTCCTCCAGAACTGATGTTGGAATTATTGATGG GCTGTCTGGACTTGCCTCCTCTGTGGATGAGTACCCAGTAGATACCATTGCGAAGAGGTTCCGCTACGAC TCTGCTTTGGTGTCTGCTTTGATGGACATGGAAGAAGACATCTTGGAAGGCATGAGATCCCAAGATCTTG ATGACTACCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGA GAAGCACGGGAGTGGGCCCGCAGTTCCAGAAAAGGCCGTTCGTTTCTCTTTCACAGTCATGAGAATTACG ATAGAGCATGGTTCACAGAACGTGAAGGTGTTTGAGGAACCCAAGCCCAATTCTGAACTGTGTTGCAAGC CGTTGTGTCTTATGCTGGCAGATGAGTCTGACCATGAGACCCTTACTGCTATTCTAAGCCCCCTCATTGC CGAGAGGGAGGCCATGAAGAGCAGTGAATTAACGCTGGAGATGGGAGGCATCCCCAGGACTTTTAAATTC ATCTTCAGGGGCACTGGATACGATGAAAAACTTGTCCGGGAAGTAGAAGGCTTGGAAGCTTCTGGCTCAG TCTACATCTGTACACTCTGTGACACCACCCGTTTGGAAGCCTCTCAGAATCTTGTCTTCCACTCCATAAC CAGAAGCCACGCCGAGAACCTGCAGCGCTATGAGGTCTGGCGGTCCAATCCGTATCATGAGTCCGTGGAA GAGCTCCGGGACCGGGTGAAAGGGGTCTCTGCCAAACCTTTCATCGAGACAGTCCCTTCCATAGATGCGC TTCACTGTGACATTGGCAATGCAGCTGAATTCTATAAGATTTTCCAGCTGGAGATAGGGGAAGTGTATAA ACATCCCAATGCCTCTAAAGAGGAAAGGAAGAGATGGCAGGCCACGCTGGACAAACATCTCCGGAAAAGG ATGAACTTAAAACCAATCATGAGGATGAATGGCAACTTTGCCCGGAAGCTTATGACCCAAGAGACTGTAG ACGCAGTTTGTGAGTTAATTCCTTCTGAGGAGAGGCATGAAGCTCTCAGGGAGCTCATGGACCTTTACCT GAAGATGAAACCCGTGTGGCGCTCTTCATGTCCCGCTAAAGAGTGTCCAGAGTCCCTCTGTCAGTACAGT TTCAACTCACAGCGTTTCGCGGAACTCCTCTCCACCAAGTTCAAATATAGATACGAGGGCAAAATCACCA ATTACTTTCACAAAACCTTGGCACATGTCCCTGAAATTATTGAAAGGGATGGCTCTATCGGGGCCTGGGC AAGTGAGGGAAATGAATCGGGTAACAAGCTGTTTAGACGGTTTCGGAAAATGAATGCCAGGCAGTCCAAG TGCTATGAGATGGAAGATGTCCTGAAACATCACTGGCTGTATACTTCAAAATACCTCCAGAAGTTTATGA ATGCTCATAACGCGTTAAAAAGCTCTGGGTTTACCATGAACTCAAAGGAGACCTTAGGGGACCCTTTGGG CATTGAGGACTCTCTGGAAAGCCAAGATTCAATGGAGTTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227208 representing NM_009019
Red=Cloning site Green=Tags(s) MAASLPSTLSFSSAPDEIQHPQIKFSEWKFKLFRVRSFEKAPEEAQKEKDSSEGKPYLEQSPVVPEKPGG QNSILTQRALKLHPKFSKKFHADGKSSDKAVHQARLRHFCRICGNRFKSDGHSRRYPVHGPVDAKTQSLF RKKEKRVTSWPDLIARIFRIDVKADVDSIHPTEFCHDCWSIMHRKFSSSHSQVYFPRKVTVEWHPHTPSC DICFTAHRGLKRKRHQPNVQLSKKLKTVLNHARRDRRKRTQARVSSKEVLKKISNCSKIHLSTKLLAVDF PAHFVKSISCQICEHILADPVETSCKHLFCRICILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLNILNS LMVKCPAQDCNEEVSLEKYNHHVSSHKESKETLVHINKGGRPRQHLLSLTRRAQKHRLRELKIQVKEFAD KEEGGDVKAVCLTLFLLALRARNEHRQADELEAIMQGRGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKA ITGRQIFQPLHALRNAEKVLLPGYHPFEWQPPLKNVSSRTDVGIIDGLSGLASSVDEYPVDTIAKRFRYD SALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSFTVMRIT IEHGSQNVKVFEEPKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELTLEMGGIPRTFKF IFRGTGYDEKLVREVEGLEASGSVYICTLCDTTRLEASQNLVFHSITRSHAENLQRYEVWRSNPYHESVE ELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKHPNASKEERKRWQATLDKHLRKR MNLKPIMRMNGNFARKLMTQETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYS FNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSK CYEMEDVLKHHWLYTSKYLQKFMNAHNALKSSGFTMNSKETLGDPLGIEDSLESQDSMEF SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009019 |
| ORF Size | 3120 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009019.2, NP_033045.2 |
| RefSeq Size | 6669 bp |
| RefSeq ORF | 3123 bp |
| Locus ID | 19373 |
| UniProt ID | P15919 |
| Gene Summary | Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223320 | Rag1 (untagged) - Mouse recombination activating gene 1 (Rag1), (10ug) |
CNY 13,400.00 |
|
| MR227208 | Rag1 (Myc-DDK-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 7,240.00 |
|
| MR227208L3 | Lenti ORF clone of Rag1 (Myc-DDK-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 10,260.00 |
|
| MR227208L4 | Lenti ORF clone of Rag1 (mGFP-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 10,260.00 |
