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Irak1 (NM_001177976) Mouse Tagged ORF Clone
CAT＃: MG227184
- TrueORF®
Irak1 (tGFP-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001177976" in other vectors (3)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA408924; Il1rak; IRAK; IRAK-1; IRAK1-S; IRAK1b; mPLK; Plpk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227184 representing NM_001177976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGGGCCGGGCCCCGGGGAGCCTGTGGTTCCAGGCGCCCAGCACTTCTTGTACGAGGTGCCAC CCTGGGTTATGTGCCGCTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGC CTTGATCGTGCGCGACCAGACAGAGCTGCGGCTGTGCGAGCGCTCCGAGCAGCGCACAGCCAGTGTTCTG TGGCCCTGGATCAACCGCAACGCGCGCGTAGCTGACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGC GTGCGAGGGACATCATCACAGCCTGGCACCCTCCTGCCCCCGTTGTGCCCCCAAGCACCGCTGCCCCAAG GCCCAGCAGCATCTCTGCAGGCTCTGAGGCCGGGGACTGGAGCCCCCGGAAATTGCAGTCCTCTGCCTCC ACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCC CTGTTTCCCTCGGGCCACCACTACCATCTTCAGCCCCTTCCTCCACCAAGTCAAGCCCAGAGAGCCCAGT GTCTGGCCTCCAAAGAGCCCATCCCTCCCCGTTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACCTGC AACTTCTCTGAAGAACTCAGGATTGGAGAGGGTGGTTTTGGATGTGTGTACCGAGCAGTCATGAGAAATA CTACATATGCTGTGAAGAGACTGAAGGAGGAAGCTGACCTAGAGTGGACTATGGTGAAACAGAGCTTCTT AACAGAGGTGGAACAGCTATCAAGGTTTCGTCACCCAAATATCGTAGACTTTGCTGGCTACTGTGCAGAG AGTGGCTTATACTGCCTTGTTTATGGCTTCTTGCCCAATGGCTCCTTGGAGGATCAGCTCCACCTTCAGA CCCAGGCCTGCTCCCCACTTTCCTGGCCTCAACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCA GTTTTTACATCAGGATAGCCCCAGCCTTATCCATGGAGACATCAAGAGTTCTAACGTGCTTCTGGATGAG AGACTGATGCCCAAGCTGGGAGACTTTGGCCTGGCTCGTTTCAGCCGCTTTGCGGGGGCCAAAGCAAGCC AGAGCAGTACTGTGGCCCGGACTTCCACAGTTCGAGGTACCCTCGCCTACTTGCCTGAGGAGTACATCAA GACAGGCCGACTGGCTGTGGACACCGATACCTTCAGCTTTGGGGTGGTAATACTGGAGACCCTTGCTGGT CAGAGGGCTGTGAGGACACAAGGTGCAAAGACCAAGTATTTGAAAGACCTGATTGAAGATGAGGCTGAAG AGGCTGGAGTGACCCTGAAAAGCACCCAGCCTACTCTGTGGGTGGGTGTAGCCACGGATGCTTGGGCTGC TCCAATTGCTGCCCAGATCTATAAGAAGCACCTGGACTCCAGACCTGGGCCCTGCCCACCCCAGTTGGGC CTGGCCCTGGCTCAACTAGCTTGCTGCTGCATGCACCGTCGGGCCAAGAAGAGGCCCCCCATGACCCAGG TATACAAGAGACTAGAAGGGCTTCAGGCAGGGCCTCCCTGGGAGCTAGAGGTTGCCGGCCATGGCTCCCC TTCCCCACAGGAGAACTCCTACATGTCTACCACTGGCAGTGCCCAGAGTGGGGATGAACCATGGCAGCCT CTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAACTCCAGAGAAGTCCCAACCAGCCAGTGG AAAGTGATGAGAGTGTTCCCGGCCTCTCTGCTACCCTGCATTCCTGGCACTTGACTCCAGGTTCCCACCC AAGCCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACCAGAGAATCAAGTGTGAGGAGT AGCCCAGGCTTCCAGCCTACAACCATGGAAGGCTCACCCACGGGCAGCTCATCCCTGCTGTCATCAGAAC CACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCTGGCTCTTTATGAAGAAGGGGT CTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGATTTCGTGGATATTGATGCTATTGGAATT GAAGCCTTCATGTCTGAATTATTCATCAATCATATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227184 representing NM_001177976
Red=Cloning site Green=Tags(s) MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVL WPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSAS TFLSPAFPGSQTHSESELLQVPLPVSLGPPLPSSAPSSTKSSPESPVSGLQRAHPSPFCWPFCEISQGTC NFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAE SGLYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDE RLMPKLGDFGLARFSRFAGAKASQSSTVARTSTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAG QRAVRTQGAKTKYLKDLIEDEAEEAGVTLKSTQPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLG LALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQSGDEPWQP LVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTTRESSVRS SPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPDFVDIDAIGI EAFMSELFINHI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177976 |
| ORF Size | 2136 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177976.1, NP_001171447.1 |
| RefSeq Size | 2478 bp |
| RefSeq ORF | 2139 bp |
| Locus ID | 16179 |
| UniProt ID | Q62406 |
| Gene Summary | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR227184 | Irak1 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant 2 |
CNY 7,130.00 |
|
| MR227184L3 | Lenti ORF clone of Irak1 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant 2 |
CNY 9,030.00 |
|
| MR227184L4 | Lenti ORF clone of Irak1 (mGFP-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant 2 |
CNY 9,030.00 |
