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Trpv1 (NM_001001445) Mouse Tagged ORF Clone
CAT＃: MG227160
- TrueORF®
Trpv1 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily V member 1 (Trpv1), (10ug)
ORF Plasmid: DDK
"NM_001001445" in other vectors (4)
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CNY 7,744.00
CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | OTRPC1; TRPV1alpha; TRPV1beta; VR-1; Vr1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227160 representing NM_001001445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAATGGGCTAGCTTAGACTCGGATGAATCTGAGCCCCCAGCCCAAGAGAACTCCTGCCCGGACC CTCCAGACAGAGACCCTAACTCCAAGCCGCCTCCAGCCAAGCCCCACATCTTTGCTACCAGGAGTCGCAC CCGGCTTTTTGGGAAGGGTGACTCAGAAGAGGCCTCTCCCATGGACTGCCCTTATGAGGAAGGCGGGCTG GCCTCCTGCCCTATCATCACCGTCAGCTCTGTTGTCACTCTCCAGAGGTCTGTGGATGGACCTACCTGTC TCAGGCAGACATCCCAGGACTCTGTCTCCACTGGTGTTGAGACGCCCCCAAGGCTCTATGATCGCAGGAG CATCTTCGACGCTGTGGCTCAGAGCAACTGCCAGGAGCTGGAGAGCCTGCTGTCCTTCCTGCAGAAGAGC AAGAAGCGCCTGACTGACAGCGAGTTCAAAGACCCAGAGACGGGAAAGACCTGTCTGCTCAAAGCCATGC TCAATCTGCACAATGGGCAGAACGACACCATTGCTCTGCTCCTGGACATTGCCCGGAAGACAGATAGCCT GAAGCAGTTTGTCAATGCCAGCTACACAGACAGCTACTACAAGGGCCAGACAGCATTACACATTGCCATT GAAAGGCGGAACATGGCACTGGTGACCCTCTTGGTGGAGAATGGAGCAGATGTCCAGGCTGCTGCTAACG GGGACTTCTTCAAGAAAACCAAAGGGAGGCCTGGCTTCTACTTTGGTGAGCTGCCCCTGTCCCTGGCTGC GTGCACCAACCAGCTGGCCATTGTGAAGTTCCTGCTGCAGAACTCCTGGCAGCCTGCAGACATCAGTGCA CGGGATTCGGTGGGCAACACGGTGCTGCACGCCCTTGTGGAGGTGGCAGATAACACAGCTGACAACACCA AGTTCGTGACAAACATGTACAACGAGATCCTGATCCTGGGGGCCAAACTCCACCCCACACTGAAGCTAGA AGAACTCACCAACAAGAAGGGGCTTACACCGCTGGCTCTGGCTGCCAGCAGTGGGAAGATTGGGGTCTTG GCCTACATTCTCCAGAGGGAGATCCACGAACCAGAGTGCCGGCACCTGTCCAGGAAGTTCACTGAATGGG CCTATGGGCCCGTGCACTCCTCCCTTTATGACCTGTCCTGCATTGACACCTGTGAGAAGAATTCAGTGCT GGAGGTGATCGCCTACAGTAGCAGTGAGACCCCCAACCGCCACGACATGCTTCTCGTGGAGCCCTTGAAC CGACTCCTGCAGGACAAGTGGGACAGATTTGTCAAGCGCATCTTCTACTTCAACTTCTTCGTCTACTGCT TGTATATGATCATCTTCACCACGGCTGCTTACTATCGGCCTGTGGAAGGCTTGCCCCCCTATAAGCTGAA TAACACCGTTGGGGACTATTTCCGTGTCACTGGAGAGATCCTGTCTGTGTCAGGAGGAGTCTACTTCTTC TTCCGAGGGATCCAGTATTTCCTGCAGAGGCGACCATCCCTCAAGAGTTTGTTTGTGGACAGCTACAGTG AGATACTTTTCTTTGTACAGTCACTGTTCATGCTGGTGTCTGTGGTACTGTACTTCAGCCATCGCAAGGA GTATGTGGCTTCCATGGTGTTCTCCCTGGCCATGGGCTGGACCAACATGCTCTACTACACCCGAGGATTC CAGCAGATGGGCATCTATGCTGTCATGATTGAGAAGATGATCCTCAGAGACCTGTGTCGGTTTATGTTCG TCTACCTCGTGTTCTTGTTTGGATTTTCCACAGCCGTAGTGACACTGATCGAGGATGGGAAGAATAACTC ACTGCCTGTGGAGTCCCCACCACACAAGTGTCGGGGATCTGCCTGCAGGCCAGGTAACTCTTACAACAGC CTGTATTCCACATGTCTGGAGCTGTTCAAGTTCACCATCGGCATGGGTGACCTGGAGTTCACCGAGAACT ATGACTTCAAGGCTGTCTTCATCATCCTGTTACTGGCCTATGTGATTCTCACCTACATCCTCCTGCTCAA CATGCTCATTGCTCTCATGGGCGAGACTGTCAACAAGATTGCACAAGAGAGCAAGAACATCTGGAAGCTG CAGCGAGCCATCACCATCCTGGATACAGAGAAGAGTTTCCTGAAGTGCATGAGGAAGGCCTTCCGCTCCG GCAAGCTGCTGCAGGTGGGGTTCACGCCGGACGGCAAGGATGACTTCCGGTGGTGCTTCAGGGTGGATGA GGTGAACTGGACTACCTGGAACACCAACGTGGGCATCATCAACGAGGACCCAGGCAACTGTGAGGGCGTC AAGCGCACCCTGAGCTTCTCCCTGCGGTCAGGCCGAGTTTCAGGGAGAAACTGGAAGAACTTTGCCCTGG TTCCCCTTCTGAGGGACGCAAGCACTCGAGATAGGCATAGCACCCAGCCGGAAGAAGTTCAGCTGAAGCA CTATACGGGATCCCTTAAGCCAGAGGATGCTGAGGTCTTCAAGGATTCCATGGCCCCAGGGGAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227160 representing NM_001001445
Red=Cloning site Green=Tags(s) MEKWASLDSDESEPPAQENSCPDPPDRDPNSKPPPAKPHIFATRSRTRLFGKGDSEEASPMDCPYEEGGL ASCPIITVSSVVTLQRSVDGPTCLRQTSQDSVSTGVETPPRLYDRRSIFDAVAQSNCQELESLLSFLQKS KKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDIARKTDSLKQFVNASYTDSYYKGQTALHIAI ERRNMALVTLLVENGADVQAAANGDFFKKTKGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADISA RDSVGNTVLHALVEVADNTADNTKFVTNMYNEILILGAKLHPTLKLEELTNKKGLTPLALAASSGKIGVL AYILQREIHEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN RLLQDKWDRFVKRIFYFNFFVYCLYMIIFTTAAYYRPVEGLPPYKLNNTVGDYFRVTGEILSVSGGVYFF FRGIQYFLQRRPSLKSLFVDSYSEILFFVQSLFMLVSVVLYFSHRKEYVASMVFSLAMGWTNMLYYTRGF QQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLIEDGKNNSLPVESPPHKCRGSACRPGNSYNS LYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKL QRAITILDTEKSFLKCMRKAFRSGKLLQVGFTPDGKDDFRWCFRVDEVNWTTWNTNVGIINEDPGNCEGV KRTLSFSLRSGRVSGRNWKNFALVPLLRDASTRDRHSTQPEEVQLKHYTGSLKPEDAEVFKDSMAPGEK TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001445 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001445.2 |
| RefSeq Size | 2520 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 193034 |
| UniProt ID | Q704Y3 |
| Gene Summary | Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli (PubMed:15194687, PubMed:15489017). Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia (PubMed:10764638). Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222020 | Trpv1 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 1 (Trpv1), (10ug) |
CNY 6,152.00 |
|
| MR227160 | Trpv1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 1 (Trpv1) |
CNY 6,144.00 |
|
| MR227160L3 | Lenti ORF clone of Trpv1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 1 (Trpv1) |
CNY 9,310.00 |
|
| MR227160L4 | Lenti ORF clone of Trpv1 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 1 (Trpv1) |
CNY 8,544.00 |
