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Pik3ca (NM_008839) Mouse Tagged ORF Clone
CAT＃: MG226580
- TrueORF®
Pik3ca (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase catalytic alpha polypeptide (Pik3ca), (10ug)
ORF Plasmid: DDK
"NM_008839" in other vectors (6)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330412C24Rik; caPI3K; p110; p110alpha |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226580 representing NM_008839
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTTGATGCCCCCACGAATCCTAGTGGAAT GTTTACTCCCCAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCCACACTCGTCACCATCAAACA TGAACTGTTCAGAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGACGAAACTTCTTACATTTTC GTAAGTGTCACCCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTGTGACCTTCGGC TTTTTCAACCCTTTTTAAAAGTTATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCGAGAAAT TGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTCCAAGACTTTCGA AGGAACATTCTGAATGTTTGCAAAGAAGCTGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAA TGTATGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCTACAACAAGTTAGATAA AGGACAAATCATAGTGGTGATTTGGGTAATAGTCTCTCCAAACAACGACAAGCAGAAGTACACTCTGAAG ATCAATCATGACTGTGTGCCAGAGCAAGTCATTGCTGAAGCCATCAGGAAAAAGACTCGGAGCATGTTGT TGTCCTCTGAGCAGCTGAAACTCTGTGTCTTAGAATATCAGGGCAAGTATATTCTGAAAGTGTGTGGCTG TGACGAATACTTCCTGGAAAAGTACCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGG AGGATGCCCAACTTGATGCTGATGGCCAAAGAAAGCCTATACTCTCAGCTGCCGATTGATAGCTTCACCA TGCCGTCATACTCCAGGCGCATCTCCACAGCCACACCCTACATGAATGGAGAGACATCTACGAAATCCCT CTGGGTCATAAATAGTGCGCTCAGAATAAAAATTCTTTGTGCAACCTATGTAAATGTAAATATTCGAGAC ATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTC AAAGAGTACCTTGTTCCAATCCTAGGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCC TCGTGCTGCGCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGCTAAGGAGGAGCACTGT CCGTTGGCCTGGGGAAACATAAACTTGTTTGATTATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGA ATCTCTGGCCTGTACCGCATGGGTTAGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAATCCAAA TAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCAGTGTGGTGAAGTTTCCAGACATGTCT GTGATCGAAGAACATGCCAATTGGTCCGTGTCCCGAGAAGCTGGATTCAGTTACTCCCATACAGGACTGA GTAACAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCCGAGCACTTTGCACCCG GGACCCACTATCTGAAATCACTGAACAAGAGAAAGACTTCCTATGGAGCCACAGACACTACTGCGTAACT ATTCCTGAAATCCTACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGACGAAGTGGCCCAGATGT ACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCCATGGAACTCCTGGACTGTAACTATCC AGATCCTATGGTTCGGAGTTTTGCTGTTCGGTGCTTAGAAAAATATTTAACAGATGACAAACTTTCTCAG TACCTCATTCAACTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTGCTTGTGAGATTTTTAC TCAAGAAAGCATTGACAAATCAAAGGATTGGCCATTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAA TAAGACTGTCAGTCAGAGGTTTGGCCTGCTATTGGAGTCCTACTGCCGTGCCTGTGGGATGTATCTGAAG CACCTGAACAGACAAGTAGAGGCCATGGAGAAGCTCATCAACCTAACGGACATCCTTAAGCAGGAGAAGA AGGATGAGACACAAAAGGTACAGATGAAGTTTTTGGTTGAACAGATGAGACAGCCAGACTTCATGGATGC TTTGCAGGGTTTTCTGTCCCCTCTGAATCCTGCTCACCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGA ATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCAGAGCTACTGT TTCAGAACAATGAGATCATCTTTAAAAATGGCGACGACTTACGGCAAGATATGTTAACCCTTCAGATCAT CCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTCGCATGCTACCTTATGGCTGTCTATCC ATTGGGGACTGTGTGGGTCTCATCGAGGTGGTGAGAAACTCTCACACCATCATGCAAATCCAGTGCAAAG GAGGCCTGAAGGGGGCGCTGCAGTTCAACAGCCACACACTGCATCAATGGCTCAAGGACAAGAACAAGGG CGAGATATATGATGCAGCCATTGACCTGTTCACTCGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATC TTGGGAATTGGAGACCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATATAGATT TTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAGCGGGAACGTGTGCCATTTGTGTTGAC ACAGGATTTCTTGATTGTGATTAGTAAGGGAGCACAAGAGTACACCAAGACCAGAGAGTTTGAGAGGTTT CAGGAGATGTGTTACAAGGCTTACCTAGCAATTCGGCAGCATGCCAATCTCTTCATCAACCTTTTTTCAA TGATGCTTGGCTCTGGAATGCCAGAACTACAATCTTTTGATGACATTGCATATATCCGAAAGACTCTAGC CTTGGACAAAACTGAGCAAGAAGCTTTGGAATATTTCACAAAGCAAATGAATGATGCACATCATGGTGGA TGGACGACAAAAATGGATTGGATCTTCCACACCATCAAGCAGCATGCTTTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226580 representing NM_008839
Red=Cloning site Green=Tags(s) MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVTIKHELFREARKYPLHQLLQDETSYIF VSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIGMPVCEFDMVKDPEVQDFR RNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLK INHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLG RMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRD IDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHC PLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMS VIEEHANWSVSREAGFSYSHTGLSNRLARDNELRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVT IPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQ YLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLK HLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECR IMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLS IGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFI LGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTREFERF QEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGG WTTKMDWIFHTIKQHALN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008839 |
| ORF Size | 3204 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008839.3 |
| RefSeq Size | 8917 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 18706 |
| UniProt ID | P42337 |
| Gene Summary | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis (PubMed:19604150).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223413 | Pik3ca (untagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca), (10ug) |
CNY 13,680.00 |
|
| MR226580 | Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 11,152.00 |
|
| MR226580L1 | Lenti ORF clone of Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 13,552.00 |
|
| MR226580L2 | Lenti ORF clone of Pik3ca (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
|
| MR226580L3 | Lenti ORF clone of Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
|
| MR226580L4 | Lenti ORF clone of Pik3ca (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
