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C3 (NM_009778) Mouse Tagged ORF Clone
CAT＃: MG226388
- TrueORF®
C3 (tGFP-tagged) - Mouse complement component 3 (C3), (10ug)
ORF Plasmid: DDK
"NM_009778" in other vectors (4)
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CNY 16,060.00
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热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI255234; ASP; HSE-MSF; Plp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226388 representing NM_009778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCAGCTTCAGGGTCCCAGCTACTAGTGCTACTGCTGCTGTTGGCCAGCTCCCCATTAGCTCTGG GGATCCCCATGTATTCCATCATTACTCCCAATGTCCTACGGCTGGAGAGCGAAGAGACCATCGTACTGGA GGCCCACGATGCTCAGGGTGACATCCCAGTCACAGTCACTGTGCAAGACTTCCTAAAGAGGCAAGTGCTG ACCAGTGAGAAGACAGTGTTGACAGGAGCCAGTGGACATCTGAGAAGCGTCTCCATCAAGATTCCAGCCA GTAAGGAATTCAACTCAGATAAGGAGGGGCACAAGTACGTGACAGTGGTGGCAAACTTCGGGGAAACGGT GGTGGAGAAAGCAGTGATGGTAAGCTTCCAGAGTGGGTACCTCTTCATCCAGACAGACAAGACCATCTAC ACCCCTGGCTCCACTGTCTTATATCGGATCTTCACTGTGGACAACAACCTACTGCCCGTGGGCAAGACAG TCGTCATCCTCATTGAGACCCCCGATGGCATTCCTGTCAAGAGAGACATTCTGTCTTCCAACAACCAACA CGGCATCTTGCCTTTGTCTTGGAACATTCCTGAACTGGTCAACATGGGGCAGTGGAAGATCCGAGCCTTT TACGAACATGCGCCGAAGCAGATCTTCTCCGCAGAGTTTGAGGTGAAGGAATACGTGCTGCCCAGTTTTG AGGTCCGGGTGGAGCCCACAGAGACATTTTATTACATCGATGACCCAAATGGCCTGGAAGTTTCCATCAT AGCCAAGTTCCTGTACGGGAAAAACGTGGACGGGACAGCCTTCGTGATTTTTGGGGTCCAGGATGGCGAT AAGAAGATTTCTCTGGCCCACTCCCTCACGCGCGTAGTGATTGAGGATGGTGTGGGGGATGCAGTGCTGA CCCGGAAGGTGCTGATGGAGGGGGTACGGCCTTCCAACGCCGACGCCCTGGTGGGGAAGTCCCTGTATGT CTCCGTCACTGTCATCCTGCACTCAGGTAGTGACATGGTAGAGGCAGAGCGCAGTGGGATCCCGATTGTC ACTTCCCCGTACCAGATCCACTTCACCAAGACACCCAAATTCTTCAAGCCAGCCATGCCCTTTGACCTCA TGGTGTTCGTGACCAACCCCGATGGCTCTCCGGCCAGCAAAGTGCTGGTGGTCACTCAGGGATCTAATGC AAAGGCTCTCACCCAAGATGATGGCGTGGCCAAGCTAAGCATCAACACACCCAACAGCCGCCAACCCCTG ACCATCACAGTCCGCACCAAGAAGGACACTCTCCCAGAATCACGGCAGGCCACCAAGACAATGGAGGCCC ATCCCTACAGCACTATGCACAACTCCAACAACTACCTACACTTGTCAGTGTCACGAATGGAGCTCAAGCC GGGGGACAACCTCAATGTCAACTTCCACCTGCGCACAGACCCAGGCCATGAGGCCAAGATCCGATACTAC ACCTACCTGGTTATGAACAAGGGGAAGCTCCTGAAGGCAGGCCGCCAGGTTCGGGAGCCTGGCCAGGACC TGGTGGTCTTGTCCCTGCCCATCACTCCAGAGTTTATTCCTTCATTTCGCCTGGTGGCTTACTACACCCT GATTGGAGCTAGTGGCCAGAGGGAGGTGGTGGCTGACTCTGTGTGGGTGGATGTGAAGGATTCCTGTATT GGCACGCTGGTGGTGAAGGGTGACCCAAGAGATAACCATCTCGCACCTGGGCAACAAACGACACTCAGGA TTGAAGGAAACCAGGGGGCCCGAGTGGGGCTAGTGGCTGTGGACAAGGGAGTGTTTGTGCTGAACAAGAA GAACAAACTCACACAGAGCAAGATCTGGGATGTGGTAGAGAAGGCAGACATTGGCTGCACCCCAGGCAGT GGGAAGAACTATGCTGGTGTCTTCATGGATGCAGGCCTGGCCTTCAAGACAAGCCAAGGACTGCAGACTG AACAGAGAGCAGATCTTGAGTGCACCAAGCCAGCAGCCCGCCGCCGTCGCTCAGTACAGTTGATGGAAAG AAGGATGGACAAAGCTGGTCAGTACACTGACAAGGGTCTTCGGAAGTGTTGTGAGGATGGTATGCGGGAT ATCCCTATGAGATACAGCTGCCAGCGCCGGGCACGCCTCATCACCCAGGGCGAGAACTGCATAAAGGCCT TCATAGACTGCTGCAACCACATCACCAAGCTGCGTGAACAACACAGAAGAGACCACGTGCTGGGCCTGGC CAGGAGTGAATTGGAGGAAGACATAATTCCAGAAGAAGATATTATCTCTAGAAGCCACTTCCCACAGAGC TGGTTGTGGACCATAGAAGAGTTGAAAGAACCAGAGAAAAATGGAATCTCTACGAAGGTCATGAACATCT TTCTCAAAGATTCCATCACCACCTGGGAGATTCTGGCAGTGAGCTTGTCAGACAAGAAAGGGATCTGTGT GGCAGACCCCTATGAGATCAGAGTGATGCAGGACTTCTTCATTGACCTGCGGCTGCCCTACTCTGTAGTG CGCAACGAACAGGTGGAGATCAGAGCTGTGCTCTTCAACTACCGTGAACAGGAGGAACTTAAGGTGAGGG TGGAACTGTTGCATAATCCAGCCTTCTGCAGCATGGCCACCGCCAAGAATCGCTACTTCCAGACCATCAA AATCCCTCCCAAGTCCTCGGTGGCTGTACCGTATGTCATTGTCCCCTTGAAGATCGGCCAACAAGAGGTG GAGGTCAAGGCTGCTGTCTTCAATCACTTCATCAGTGATGGTGTCAAGAAGACACTGAAGGTCGTGCCAG AAGGAATGAGAATCAACAAAACTGTGGCCATCCATACACTGGACCCAGAGAAGCTCGGTCAAGGGGGAGT GCAGAAGGTGGATGTGCCTGCCGCAGACCTTAGCGACCAAGTGCCAGACACAGACTCTGAGACCAGAATT ATCCTGCAAGGGAGCCCGGTGGTTCAGATGGCTGAAGATGCTGTGGACGGGGAGCGGCTGAAACACCTGA TCGTGACCCCCGCAGGCTGTGGGGAACAGAACATGATTGGCATGACACCAACAGTCATTGCGGTACACTA CCTGGACCAGACCGAACAGTGGGAGAAGTTCGGCATAGAGAAGAGGCAAGAGGCCCTGGAGCTCATCAAG AAAGGGTACACCCAGCAGCTGGCCTTCAAACAGCCCAGCTCTGCCTATGCTGCCTTCAACAACCGGCCCC CCAGCACCTGGCTGACAGCCTACGTGGTCAAGGTCTTCTCTCTAGCTGCCAACCTCATCGCCATCGACTC TCACGTCCTGTGTGGGGCTGTTAAATGGTTGATTCTGGAGAAACAGAAGCCGGATGGTGTCTTTCAGGAG GATGGGCCCGTGATTCACCAAGAAATGATTGGTGGCTTCCGGAACGCCAAGGAGGCAGATGTGTCACTCA CAGCCTTCGTCCTCATCGCACTGCAGGAAGCCAGGGACATCTGTGAGGGGCAGGTCAATAGCCTTCCTGG GAGCATCAACAAGGCAGGGGAGTATATTGAAGCCAGTTACATGAACCTGCAGAGACCATACACAGTGGCC ATTGCTGGGTATGCCCTGGCCCTGATGAACAAACTGGAGGAACCTTACCTCGGCAAGTTTCTGAACACAG CCAAAGATCGGAACCGCTGGGAGGAGCCTGACCAGCAGCTCTACAACGTAGAGGCCACATCCTACGCCCT CCTGGCCCTGCTGCTGCTGAAAGACTTTGACTCTGTGCCCCCTGTAGTGCGCTGGCTCAATGAGCAAAGA TACTACGGAGGCGGCTATGGCTCCACCCAGGCTACCTTCATGGTATTCCAAGCCTTGGCCCAATATCAAA CAGATGTCCCTGACCATAAGGACTTGAACATGGATGTGTCCTTCCACCTCCCCAGCCGTAGCTCTGCAAC CACGTTTCGCCTGCTCTGGGAAAATGGCAACCTCCTGCGATCGGAAGAGACCAAGCAAAATGAGGCCTTC TCTCTAACAGCCAAAGGAAAAGGCCGAGGCACATTGTCGGTGGTGGCAGTGTATCATGCCAAACTCAAAA GCAAAGTCACCTGCAAGAAGTTTGACCTCAGGGTCAGCATAAGACCAGCCCCTGAGACAGCCAAGAAGCC CGAGGAAGCCAAGAATACCATGTTCCTTGAAATCTGCACCAAGTACTTGGGAGATGTGGACGCCACTATG TCCATCCTGGACATCTCCATGATGACTGGCTTTGCTCCAGACACAAAGGACCTGGAACTGCTGGCCTCTG GAGTAGATAGATACATCTCCAAGTACGAGATGAACAAAGCCTTCTCCAACAAGAACACCCTCATCATCTA CCTAGAAAAGATTTCACACACCGAAGAAGACTGCCTGACCTTCAAAGTTCACCAGTACTTTAATGTGGGA CTTATCCAGCCCGGGTCGGTCAAGGTCTACTCCTATTACAACCTCGAGGAATCATGCACCCGGTTCTATC ATCCAGAGAAGGACGATGGGATGCTCAGCAAGCTGTGCCACAGTGAAATGTGCCGGTGTGCTGAAGAGAA CTGCTTCATGCAACAGTCACAGGAGAAGATCAACCTGAATGTCCGGCTAGACAAGGCTTGTGAGCCCGGA GTCGACTATGTGTACAAGACCGAGCTAACCAACATAGAGCTGTTGGATGATTTTGATGAGTACACCATGA CCATCCAGCAGGTCATCAAGTCAGGCTCAGATGAGGTGCAGGCAGGGCAGCAACGCAAGTTCATCAGCCA CATCAAGTGCAGAAACGCCCTGAAGCTGCAGAAAGGGAAGAAGTACCTCATGTGGGGCCTCTCCTCTGAC CTCTGGGGAGAAAAGCCCAACACCAGCTACATCATTGGGAAGGACACGTGGGTGGAGCACTGGCCTGAGG CAGAAGAATGCCAGGATCAGAAGTACCAGAAACAGTGCGAAGAACTTGGGGCATTCACAGAATCTATGGT GGTTTATGGTTGTCCCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226388 representing NM_009778
Red=Cloning site Green=Tags(s) MGPASGSQLLVLLLLLASSPLALGIPMYSIITPNVLRLESEETIVLEAHDAQGDIPVTVTVQDFLKRQVL TSEKTVLTGASGHLRSVSIKIPASKEFNSDKEGHKYVTVVANFGETVVEKAVMVSFQSGYLFIQTDKTIY TPGSTVLYRIFTVDNNLLPVGKTVVILIETPDGIPVKRDILSSNNQHGILPLSWNIPELVNMGQWKIRAF YEHAPKQIFSAEFEVKEYVLPSFEVRVEPTETFYYIDDPNGLEVSIIAKFLYGKNVDGTAFVIFGVQDGD KKISLAHSLTRVVIEDGVGDAVLTRKVLMEGVRPSNADALVGKSLYVSVTVILHSGSDMVEAERSGIPIV TSPYQIHFTKTPKFFKPAMPFDLMVFVTNPDGSPASKVLVVTQGSNAKALTQDDGVAKLSINTPNSRQPL TITVRTKKDTLPESRQATKTMEAHPYSTMHNSNNYLHLSVSRMELKPGDNLNVNFHLRTDPGHEAKIRYY TYLVMNKGKLLKAGRQVREPGQDLVVLSLPITPEFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCI GTLVVKGDPRDNHLAPGQQTTLRIEGNQGARVGLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGS GKNYAGVFMDAGLAFKTSQGLQTEQRADLECTKPAARRRRSVQLMERRMDKAGQYTDKGLRKCCEDGMRD IPMRYSCQRRARLITQGENCIKAFIDCCNHITKLREQHRRDHVLGLARSELEEDIIPEEDIISRSHFPQS WLWTIEELKEPEKNGISTKVMNIFLKDSITTWEILAVSLSDKKGICVADPYEIRVMQDFFIDLRLPYSVV RNEQVEIRAVLFNYREQEELKVRVELLHNPAFCSMATAKNRYFQTIKIPPKSSVAVPYVIVPLKIGQQEV EVKAAVFNHFISDGVKKTLKVVPEGMRINKTVAIHTLDPEKLGQGGVQKVDVPAADLSDQVPDTDSETRI ILQGSPVVQMAEDAVDGERLKHLIVTPAGCGEQNMIGMTPTVIAVHYLDQTEQWEKFGIEKRQEALELIK KGYTQQLAFKQPSSAYAAFNNRPPSTWLTAYVVKVFSLAANLIAIDSHVLCGAVKWLILEKQKPDGVFQE DGPVIHQEMIGGFRNAKEADVSLTAFVLIALQEARDICEGQVNSLPGSINKAGEYIEASYMNLQRPYTVA IAGYALALMNKLEEPYLGKFLNTAKDRNRWEEPDQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQR YYGGGYGSTQATFMVFQALAQYQTDVPDHKDLNMDVSFHLPSRSSATTFRLLWENGNLLRSEETKQNEAF SLTAKGKGRGTLSVVAVYHAKLKSKVTCKKFDLRVSIRPAPETAKKPEEAKNTMFLEICTKYLGDVDATM SILDISMMTGFAPDTKDLELLASGVDRYISKYEMNKAFSNKNTLIIYLEKISHTEEDCLTFKVHQYFNVG LIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHSEMCRCAEENCFMQQSQEKINLNVRLDKACEPG VDYVYKTELTNIELLDDFDEYTMTIQQVIKSGSDEVQAGQQRKFISHIKCRNALKLQKGKKYLMWGLSSD LWGEKPNTSYIIGKDTWVEHWPEAEECQDQKYQKQCEELGAFTESMVVYGCPN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009778 |
| ORF Size | 4989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009778.3 |
| RefSeq Size | 5147 bp |
| RefSeq ORF | 4992 bp |
| Locus ID | 12266 |
| UniProt ID | P01027 |
| Gene Summary | This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224797 | C3 (untagged) - Mouse complement component 3 (C3), (10ug) |
CNY 11,384.00 |
|
| MR226388 | C3 (Myc-DDK-tagged) - Mouse complement component 3 (C3) |
CNY 11,376.00 |
|
| MR226388L3 | Lenti ORF clone of C3 (Myc-DDK-tagged) - Mouse complement component 3 (C3) |
CNY 16,530.00 |
|
| MR226388L4 | Lenti ORF clone of C3 (mGFP-tagged) - Mouse complement component 3 (C3) |
CNY 16,530.00 |
