United States
Germany
Japan
United Kingdom
China
Traf3 (NM_001048206) Mouse Tagged ORF Clone
CAT＃: MG225762
- TrueORF®
Traf3 (tGFP-tagged) - Mouse TNF receptor-associated factor 3 (Traf3) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001048206" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1; T-BAM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225762 representing NM_001048206
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAAGCAAAAAGATGGATGCTGCTGGCACACTGCAGCCTAACCCACCCCTAAAGCTGCAGCCTG ATCGCGGCGCAGGGTCCGTGCTCGTGCCGGAGCAAGGAGGCTACAAGGAGAAGTTTGTGAAGACGGTGGA AGACAAGTACAAGTGCGAGAAGTGCCGCCTGGTGCTGTGCAACCCGAAGCAGACGGAGTGTGGCCACCGG TTCTGCGAGAGCTGCATGGCCGCCCTGCTGAGCTCCTCCAGTCCAAAATGCACAGCGTGCCAAGAAAGCA TCATCAAAGACAAGGTGTTTAAGGATAATTGCTGCAAGAGAGAGATTCTGGCCCTTCAGGTCTACTGTCG GAATGAAGGCAGAGGTTGTGCGGAGCAGCTGACTCTGGGACATCTGCTGGTGCACCTAAAAAATGAATGT CAGTTTGAGGAACTTCCCTGTCTGCGTGCCGACTGCAAAGAAAAAGTACTGAGAAAAGACTTGCGGGATC ACGTGGAAAAGGCCTGTAAATACCGCGAGGCCACGTGCAGTCACTGCAAGAGCCAAGTGCCCATGATCAA ACTGCAGAAACATGAAGACACAGATTGTCCCTGTGTGGTGGTATCCTGCCCTCACAAGTGCAGCGTTCAG ACTCTTCTAAGGAGTGAGGGTACAAACCAGCAGATCAAGGCCCATGAGGCCAGCTCCGCGGTACAGCACG TGAACCTGCTGAAGGAGTGGAGCAACTCCCTGGAGAAGAAGGTTTCCCTGCTGCAGAATGAAAGTGTTGA GAAAAACAAGAGCATCCAAAGCCTGCACAACCAGATCTGCAGCTTTGAGATCGAGATTGAGAGGCAGAAG GAGATGCTCCGAAACAACGAGTCCAAGATCCTTCACCTGCAGCGGGTAATCGACAGCCAAGCAGAGAAAC TGAAAGAACTGGACAAGGAGATCCGTCCCTTCCGGCAGAACTGGGAGGAAGCGGACAGCATGAAGAGCAG TGTGGAGTCCCTCCAGAACCGAGTGACTGAGCTGGAGAGCGTAGACAAAAGTGCGGGGCAGGCGGCTCGC AACACAGGCTTGCTGGAGTCCCAGCTGAGCCGGCATGACCAGATGTTGAGTGTTCATGACATCCGCTTGG CCGACATGGACCTGCGGTTCCAGGTCCTCGAGACCGCCAGCTACAACGGGGTGCTGATCTGGAAGATCCG TGACTACAAGCGCCGGAAGCAGGAGGCCGTCATGGGGAAGACCCTGTCTCTCTACAGCCAGCCTTTCTAC ACAGGTTATTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAATGGGGACGGAATGGGGAAAGGGACAC ACTTGTCGCTGTTTTTTGTCATTATGCGTGGAGAATATGATGCTCTGTTGCCATGGCCGTTCAAGCAGAA AGTGACACTTATGCTGATGGATCAGGGGTCCTCTCGCCGTCATCTGGGAGATGCGTTCAAGCCTGACCCC AACAGCAGCAGCTTCAAGAAACCCACCGGAGAGATGAATATCGCCTCTGGCTGCCCAGTCTTTGTCGCCC AAACTGTTCTAGAGAACGGGACGTATATTAAAGATGATACAATCTTTATTAAGGTCATAGTGGATACCTC GGATCTGCCTGACCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225762 representing NM_001048206
Red=Cloning site Green=Tags(s) MESSKKMDAAGTLQPNPPLKLQPDRGAGSVLVPEQGGYKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHR FCESCMAALLSSSSPKCTACQESIIKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNEC QFEELPCLRADCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIKLQKHEDTDCPCVVVSCPHKCSVQ TLLRSEGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQK EMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQAAR NTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFY TGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDP NSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001048206 |
| ORF Size | 1626 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001048206.1, NP_001041671.1 |
| RefSeq Size | 6906 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 22031 |
| Gene Summary | Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218259 | Traf3 (untagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2, (10ug) |
CNY 6,940.00 |
|
| MR225762 | Traf3 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 |
CNY 6,056.00 |
|
| MR225762L3 | Lenti ORF clone of Traf3 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 |
CNY 7,320.00 |
|
| MR225762L4 | Lenti ORF clone of Traf3 (mGFP-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 |
CNY 7,320.00 |
